A kind of fusion protein and its use for treating diseases

A technology of fusion protein and application, which is applied in the field of fusion protein and its treatment of diseases, and can solve problems such as complicated diagnosis, long course of treatment, and no medicine available

Active Publication Date: 2022-03-29
中国科学院上海免疫与感染研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the diagnosis of drug-resistant tuberculosis is complicated, the treatment is difficult, and the course of treatment is often very long. It usually takes 18-24 months for multidrug-resistant patients, and the medical expenses are about 100 times that of treating ordinary patients. Therefore, the treatment of drug-resistant tuberculosis is of great importance to individuals and families. huge economic pressure on society and
[0004] The existing chemical drug treatment of tuberculosis can easily induce drug resistance of Mycobacterium tuberculosis, and the clinical treatment of drug-resistant tuberculosis is very difficult, and severe cases even have no drugs available

Method used

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  • A kind of fusion protein and its use for treating diseases
  • A kind of fusion protein and its use for treating diseases
  • A kind of fusion protein and its use for treating diseases

Examples

Experimental program
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Embodiment 1

[0155] In this example, the inventors prepared the fusion protein of interleukin-2 and diphtheria toxin (hereinafter referred to as fusion protein) according to the present invention. For issues such as the expression vector construction of the fusion protein of the present invention and the expression and purification of the fusion protein, refer to the paper (Structure / Function Analysis of the Transmembrane Domain of DAB389-Interleukin-2, an Interleukin-2 Receptor-targeted Fusion Toxin, The journal of biological chemistry, Vol.268, No.16, pp.12077-12062, 1993).

[0156] The cDNA gene sequence of the fusion protein in the present embodiment is as follows,

[0157]

[0158] The purified fusion protein was adjusted to a concentration of 150UG / ML for animal experiments. The experiment included two groups, the first group was the normal saline control group, and the second group was the fusion protein treatment group. Each group includes 12 cynomolgus macaques, about 4-6 yea...

Embodiment 2

[0159] Example 2 fusion protein can cause apoptosis

[0160] Laser confocal technology was used to detect and found that the treatment of fusion protein could cause the apoptosis of human lung epithelial cells A549 cells that had swallowed Mycobacterium tuberculosis ( figure 2 ), further capable of killing intracellular parasitic Mycobacterium tuberculosis.

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Abstract

The invention provides a fusion protein and its use for treating tuberculosis. The fusion protein can specifically induce apoptosis of macrophages that phagocytose Mycobacterium tuberculosis, thereby killing endoparasites without inducing bacterial drug resistance . Therefore, the treatment of tuberculosis, especially drug-resistant tuberculosis, will play an important role.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular, the invention relates to a fusion protein and its application for treating diseases. Background technique [0002] The causative bacterium of tuberculosis is Mycobacterium tuberculosis (Mycobacterium tuberculosis), which is mainly divided into human type and bovine type. Mycobacterium tuberculosis infection has the highest incidence rate. Clinically referred to tuberculosis mostly caused by the above two types. Tuberculosis is mainly transmitted through the respiratory tract, and can also be infected through the digestive tract, and a few are infected through skin wounds. Respiratory transmission is the most common and important route. Tuberculosis patients (mainly cavitary tuberculosis) excrete a large number of bacteria-carrying droplets from the respiratory tract. Infection can be caused by inhalation of these bacteria-carrying droplets. Droplets with a diameter of less than 5 μm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/20A61K38/16A61P31/04A61P37/04A61P31/06C12N5/0786A61K31/4409A61K31/496A61K31/133A61K31/7036
Inventor 沈洪波陈维政张卓然陈颖黄丹姚淑瑜沈玲
Owner 中国科学院上海免疫与感染研究所
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