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Application of tabersonine in preparing medicines for preventing and treating cancers

A technology of itaponin and drugs, applied in the direction of drug combination, antineoplastic drugs, etc.

Inactive Publication Date: 2017-04-26
厦门华侨亚热带植物引种园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the prior art, there is no report that Boning can prevent and treat cancer

Method used

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  • Application of tabersonine in preparing medicines for preventing and treating cancers
  • Application of tabersonine in preparing medicines for preventing and treating cancers
  • Application of tabersonine in preparing medicines for preventing and treating cancers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Determination of cytotoxic activity

[0034] test methods:

[0035] Dissolve Tabonin with DMSO first, and then prepare solutions with concentrations of 6, 12, 18, 24, 30 and 36 μmol / L with the medium, and the solutions are used as sample determination solutions. Liver cancer cell lines SMMC7721, liver cancer cell line Bel7402, gastric cancer cell line SGC7901, ovarian cancer cell line SKOV3, and ovarian cancer cell line Hey in the logarithmic growth phase were inoculated into 96-well plates at 2500 cell lines per well. %CO 2 Incubate at 37° C. for 24 hours in an incubator, and then add 200 μL of the culture medium containing the above-mentioned different concentration gradient sample measurement solutions to continue the cultivation. There were 4 duplicate wells in both the Jiatabonin group and the negative control group, and 8 blank control wells in each plate. The blank control group did not add cancer cells and Tabonin only added the same amount of medium, and t...

Embodiment 2

[0046] Phase Contrast Microscopy and AO / EB for Cancer Cell Staining

[0047] According to the MTT assay, Taponin has obvious inhibitory effect on the proliferation of different cancer cells. To further analyze whether this inhibitory effect is due to cell necrosis or induction of apoptosis, it was confirmed from cell morphology. After being treated with Tabonin at a concentration of 6 or 24 μmol / L for 48 hours, phase contrast microscopic observation revealed that the cells shrank and became smaller, accompanied by nuclear pyknosis, a large number of cell fragments appeared in the culture medium, and the cells presented typical apoptotic bodies . In the control group, the cells were full and stretched, adhered to the wall and grew in sheets; the apoptotic cells stained by AO / EB showed orange-red fluorescence or enhanced staining, and the fluorescence was brighter, with a uniform round or pyknotic, mass-like structure , non-apoptotic nuclei exhibited structure-like features of...

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Abstract

The invention provides an application of tabersonine in preparing medicines for preventing and treating cancers. The tabersonine can be used for effectively preventing and treating the cancers. According to the invention, the tabersonine is used for preventing the cancers on the basis of the principle that the tabersonine has an obvious inhibitory effect on proliferation of cancer cells, so that the cancer cells become shrunken and diminished in volume, accompanied with karyopyknosis, and finally, cancer cell apoptosis is achieved. Based upon a cytotoxicity test, IC50 value of an inhibitory effect of the tabersonine on a hepatoma cell line SMMC7721 is 12.866-13.230 [mu]mol / L and IC50 value of an inhibitory effect of the tabersonine on a hepatoma cell line Bel7402 is 14.810-15.342 [mu]mol / L, IC50 value of an inhibitory effect on a gastric carcinoma cell line SGC7901 is 11.696-11.910 [mu]mol / L, IC50 value of an inhibitory effect on an ovarian carcinoma cell line SKOV3 is 24.383-24.703 [mu]mol / L and IC50 value of an inhibitory effect on an ovarian carcinoma cell line Hey is 14.669-14.977 [mu]mol / L, showing that the tabersonine has an excellent effect of preventing and treating the cancers.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of bonin in the preparation of medicines for preventing and treating cancer. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. According to the data released by the Ministry of Health of my country, the death rate of malignant tumors in both rural and urban populations in China ranked first in 2006. Among many malignant tumors, liver cancer, gastric cancer, and ovarian cancer are the most difficult to treat. So far, there is still a lack of ideal therapeutic drugs. The mortality rate of patients remains high all year round, and the 5-year survival rate is low. 30-50% of newly discovered liver cancer, gastric cancer and ovarian cancer patients in the world every year occur in China, and most of them are 40-50 years old. Liver cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/437A61P35/00
CPCA61K31/437
Inventor 郑志忠明艳林苏丽辉陈良华李旭丹刘韶松
Owner 厦门华侨亚热带植物引种园
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