22 results about "Cell necrosis" patented technology

This invention discloses a virulent strain of type I canine adenovirus and its application in constructing animal infection models, belonging to the field of biotechnology. The virulent strain of type I canine adenovirus is named canine adenovirus type 1 strain 0601, with accession number CCTCC NO: V202604. The virus titer reaches 10. 7.5 TCID 50 / mL. This virus can be used to construct an animal model of canine adenovirus type I infection, which exhibits typical clinical symptoms, such as hepatic hemorrhage, hepatocellular necrosis, and venous and sinusoidal congestion. The constructed animal model can be used for future canine adenovirus vaccine development, immune protection assessment, and efficacy evaluation of preventive and therapeutic drugs, providing excellent biological materials and theoretical basis for further control of canine adenovirus type I.

The present invention provides a 2-substituted azole cell necrosis pathway inhibitor, a preparation method therefor and a use thereof. Specifically, the present invention provides a compound having a structure represented by formula I, each group being as defined in the description. The compound of the present invention has strong inhibitory activity against RIPK3 and a RIPK3-mediated cell necrosis pathway at both enzyme level and cell level. Therefore, the compound provided by the present invention has broad application prospects in the field of treatment or prevention of programmed cell necrosis and inflammation-related diseases.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

The invention relates to the technical field of cell culture, discloses an integral bubble-eliminating organ chip, and aims to solve the problems of cell necrosis and experiment failure caused by bubbles in a perfusion process of an existing organ chip. The chip comprises a sealing cover plate, a substrate, a bubble trapping mechanism and an attachment mechanism, bubbles in perfusate are efficiently eliminated through the bubble trapping mechanism, the attachment mechanism provides a stable attachment surface for cells, bidirectional circulation of the perfusate is achieved, and a guide-in opening and a guide-out opening do not need to be distinguished. The bubble elimination rate reaches 99.9%, the cell survival rate is improved to 95% or above, the repeatability of experimental data is improved by 80%, expensive peripherals are not needed, the use threshold of an organ chip platform is lowered, and the method is suitable for long-term and high-throughput cell culture and organ function simulation experiments.

The invention provides preparation and application of a kidney dual-targeting black phosphorus nano platform for treating acute kidney injury. Firstly, black phosphorus nanosheets and NH2-PEG-NH2 are mixed and then dispersed into deionized water, centrifugation is repeated, supernate is discarded, and a BP-PEG solution is obtained; kim-1 targeted polypeptide LTH powder is dissolved in deionized water, then EDC and NHS are added for a light-shielding reaction, a BP-PEG solution is added, light-shielding stirring is continued to obtain a BP-PEG-LTH mixture, then 4-octyl itaconic acid is added, then a PBS solution is added, light-shielding magnetic stirring is carried out, and the kidney double-targeting black phosphorus nano platform is obtained. The kidney dual-targeting black phosphorus nano platform is used as an intravenous injection for treating acute kidney injury, oxidative stress can be rapidly and effectively inhibited, inflammation is resisted, intercellular burial is promoted, malignant development of AKI is inhibited to a great extent, and the purpose of short-term specific intervention treatment after AKI is achieved.

This invention relates to a formulation and method of application of a multicomponent mycotoxin detoxifier for poultry feed, particularly female broiler chickens, which aims to provide protection to the Bursa Fabricius due to exposure to mycotoxins in the form of aflatoxin and ochratoxin. The mycotoxin detoxifier formulation according to this invention consists of an inorganic mycotoxin binder in the form of bentonite and / or zeolite, an organic mycotoxin binder in the form of pectin and / or esterified cellulose, and a natural antioxidant in the form of vitamin E and / or antioxidant herbal extracts. The formulation is applied to poultry feed at a dose of 2 to 2.5 g per kg of feed and is able to bind aflatoxin and ochratoxin simultaneously in the digestive tract, thereby reducing the bioavailability of mycotoxins.The application of this formulation has been proven to reduce histopathological damage to the Bursa of Fabricius, including lymphoid follicle depletion, cyst formation, cell necrosis, inflammatory cell infiltration, and tissue fibrosis, while also supporting the immune system function of female broiler chickens. This invention provides an effective, efficient, and easily applicable technical solution in the poultry farming industry to address mixed mycotoxin contamination in feed.

The implantable device disclosed by the invention is provided with at least one corrodible zinc-containing part, and the zinc content in the at least one zinc-containing part is within the range of [30,000, 3,000, 3,000]. The zinc content range of at least one of the zinc-containing parts is [50, 70] wt.%, the zinc in the zinc-containing parts is an amorphous structure, or the zinc content range of at least one of the zinc-containing parts is [50, 70] wt.%, and the microstructure of the zinc in the zinc-containing parts is selected from at least one of an amorphous structure, a non-equiaxed structure, an ultra-fine structure or an equiaxed structure with the grain size range of 7-14 grades, or the microstructure of the zinc in the zinc-containing parts is selected from at least one of an amorphous structure, a non-equiaxed structure, an ultra-fine structure or an equiaxed structure with the grain size range of 7-14 grades. The zinc content range of at least one zinc-containing part is (70, 100) wt.%, and the microstructure of zinc in the zinc-containing part is selected from at least one of a non-equiaxed crystal structure, an ultra-fine crystal structure or an equiaxed crystal structure with the grain size range of 7-14 grades. After the implantable instrument is implanted into a body, a zinc corrosion product is generated, and the concentration of the zinc corrosion product in the surrounding tissue of the zinc-containing part of the implantable instrument can inhibit smooth muscle cell proliferation and cannot cause normal tissue cell necrosis.

The invention discloses a liver single-cell necrosis intelligent identification method and system based on deep learning and a storage medium, and the method comprises the steps: obtaining and segmenting a WSI labeling file obtained after labeling a single-cell necrosis lesion in a liver WSI image, and obtaining Tile and Mask files; generating a training set and a test set; constructing a ResUNet model based on an encoder-decoder symmetric architecture, and training the ResUNet model to obtain an identification model; a weight based on a positive pixel ratio is introduced in the training process and is used for amplifying positive sample loss; evaluating and verifying the identification model by using the test set to obtain a trained identification model; and packaging and deploying the trained recognition model, performing intelligent recognition on the input WSI image, and outputting a single-cell necrosis lesion recognition result. By means of the method, interactive instant reasoning feedback and result visualization can be achieved, and an efficient, accurate and standardized technical solution is provided for toxicity pathology assessment.

The application belongs to the technical field of genetic engineering and molecular breeding, and particularly relates to application of TaHPPD protein in regulation of wheat stripe rust resistance. Moro The TaHPPD protein can weaken active oxygen (ROS) accumulation and cell necrosis reaction triggered by CNL class protein NLR, and reduce ion leakage. The application obtains TaHPPD knockout (TaHPPD-KO) wheat material by knocking out TaHPPD gene in wheat through gene editing technology, and obtains wheat with wheat stripe rust resistance. The results of the embodiment show that, compared with the control Fielder, the TaHPPD-KO wheat plant shows significant resistance. The TaHPPD protein can be used to regulate wheat stripe rust resistance, and can be used for cultivating wheat resistant to stripe rust.

This paper describes a class of dyes containing 1,4-diazabicyclo[2.2.2]octane, their preparation methods, and applications, belonging to the fields of optical diagnostics and biomedicine. The dye comprises the dye molecule and 1,4-diazabicyclo[2.2.2]octane covalently coupled to the dye. 1,4-diazabicyclo[2.2.2]octane directly quenches singlet oxygen generated by the dye, affecting the triplet state of the dye, thereby improving its anti-bleaching ability and photostability, and reducing phototoxicity during fluorescence imaging. Furthermore, the introduction of 1,4-diazabicyclo[2.2.2]octane significantly improves the brightness of the dye, inhibits photoscintillation, and enhances its water solubility. Its protonation characteristic enhances the dye's mitochondrial targeting ability. These compounds, by reducing singlet oxygen and enhancing the local photothermal and reactive oxygen species production in mitochondria, induce potent cell necrosis, apoptosis, and immunogenic cell death, comprehensively improving anticancer efficacy. These compounds exhibit excellent photostability and low skin toxicity, showing broad application prospects.

This invention discloses the disease resistance function and application of the CC domain of CNL protein 2541 involved in wheat stripe rust resistance, belonging to the field of functional gene technology. This invention provides the application of the 2541-CC gene domain in regulating wheat stripe rust resistance, and provides a recombinant expression vector overexpressing the 2541-CC gene domain, as well as a method for constructing plant germplasm overexpressing the 2541-CC gene domain using the recombinant expression vector. This invention found that plants overexpressing the 2541-CC gene domain exhibited significant resistance, increased leaf hypersensitive necrosis, reduced stripe rust urediniospores, increased reactive oxygen species, increased cell necrosis, and inhibited stripe rust growth and development, thus confirming that the 2541-CC gene domain is a key domain in the 2541 gene's disease resistance signal transduction.

This invention belongs to the field of genetic engineering technology and discloses the application of the transcription factor TaNAC67 in regulating wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown in SEQ ID NO: 1. This transcription factor can induce cell necrosis and plays a positive regulatory role in the interaction between wheat and stripe rust fungus. The gene encoding this transcription factor... TaNAC67 This is a disease resistance-related gene that is strongly induced by stripe rust infection in the incompatible interaction between plants and stripe rust fungi. Its nucleotide sequence is shown in SEQ ID NO: 2. Based on these characteristics, this invention further obtains an overexpressing gene. TaNAC67 The transgenic plants were tested and found to exhibit resistance to stripe rust. This invention provides genetic resources and technical support for the subsequent genetic improvement of wheat materials resistant to stripe rust.

The application discloses a liver single-cell necrosis intelligent identification method and system based on deep learning and a storage medium. The method comprises the following steps: acquiring a WSI annotation file after single-cell necrosis lesions in a liver WSI image are annotated and cutting, acquiring Tile and Mask files; generating a training set and a test set; constructing a ResUNet model based on an encoder-decoder symmetric architecture and training to obtain an identification model; introducing a weight based on a positive pixel ratio in the training process, which is used to amplify the positive sample loss; after the test set is used to evaluate and verify the identification model, a trained identification model is obtained; after the trained identification model is packaged and deployed, the input WSI image is intelligently identified, and a single-cell necrosis lesion identification result is output. Through the application, interactive instant inference feedback and visual results can be realized, and an efficient, accurate and standardized technical solution is provided for toxicity pathological evaluation.

Provided in the present invention are inhibitors for programmed cell necrosis, a preparation method therefor and a use thereof. Specifically, provided in the present invention are a compound represented by formula I and a composition comprising same. The described compound may be used to prepare a pharmaceutical composition for the prevention and / or treatment of diseases involving cell death and / or inflammation.

The present invention provides a nucleic acid molecule capable of inhibiting the expression of thymic stromal lymphopoietin (TSLP) or a gene encoding the TSLP, a salt thereof, a solvate thereof, a pharmaceutical composition characterized by containing the same as active ingredients, and a prophylactic and / or therapeutic agent for diseases in which the involvement of TSLP is presumed. The present invention relates to: a nucleic acid molecule capable of inhibiting the expression of thymic stromal lymphopoietin (TSLP) or a gene encoding the TSLP; and a pharmaceutical composition characterized by containing the same as an active ingredient.

The invention belongs to the technical field of gene engineering, and relates to application of an apple pattern recognition receptor gene MdRE02 in regulation and control of plant disease resistance. The nucleotide sequence of the apple pattern recognition receptor gene MdRE02 is as shown in SEQ ID NO.1, the MdRE02 gene is silenced in apples, the expression of an immune gene induced by a pathogen-related molecular pattern VmE02 is remarkably reduced, and the disease resistance to valsa mali of apple trees is remarkably weakened. Through overexpression of the MdRE02 gene in the tobacco mutant re02, cell necrosis and active oxygen bursting induced by the VmE02 can be recovered, which indicates that the MdRE02 is a pattern recognition receptor for recognizing the VmE02. By overexpressing the MdRE02 gene in tobacco mutants re02 and oilseed rape, the resistance of the MdRE02 gene to sclerotinia sclerotiorum can be remarkably improved. The results show that the MdRE02 gene can be used for disease-resistant breeding and disease prevention and control of valsa ceratosperma and sclerotinia sclerotiorum of apple trees.

The application discloses a banana burrowing nematode branch acid mutase and application thereof. The application provides application of a banana burrowing nematode branch acid mutase gene RsCM in prevention and treatment of banana burrowing nematodes, and the nucleotide sequence of the banana burrowing nematode branch acid mutase gene RsCM is shown in SEQ ID NO. 1 or SEQ ID NO. 2. Tobacco transient expression shows that RsCM can significantly inhibit flg22-induced defense gene expression and callose deposition, and can significantly inhibit BAX-induced cell necrosis, which indicates that RsCM can inhibit the defense response of plants; a transgenic tomato is constructed through in planta RNAi technology, and it is found that the pathogenicity of banana burrowing nematodes is reduced after inoculation of the nematodes. The application provides a new strategy for prevention and treatment of nematodes in agricultural production, provides a new target for cultivation of nematode-resistant plant varieties, and provides a new approach for prevention and treatment of banana burrowing nematodes.

A method of treating a blood vessel in a subject comprises the steps of advancing a catheter device comprising a deployable abrasive element distally across a treatment zone in the vessel, deploying the abrasive element into contact with the vessel wall, actuating the abrasive element to cause disruption of the vessel wall and exposure of subendothelial layers of the vessel wall at the treatment zone, infusing a sclerosant agent into the blood vessel via the catheter to contact and induce cellular necrosis in the exposed subendothelial layers of the vessel wall, recapturing the abrasive element by the catheter device, and withdrawing the catheter device from the vessel. The method may comprise an additional step of inducing vasospasm of the treatment zone of the vessel prior to disruption of the vessel wall by administering a stimulus to the vessel wall by the catheter device.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an RNA interference fragment of a targeted Pt15401 gene and a related biological material of the RNA interference fragment to improvement of leaf rust resistance of crops. The invention finds that Pt15401 is a typical secretory protein, and heterologous expression in tobacco finds that Pt15401 can inhibit Bax-induced cell necrosis and exert a cytotoxic function. Furthermore, the Pt15401 gene is interfered based on a host-induced gene silencing (HIGS) technology to obtain an instantaneous silent crop plant, and virus inoculation verification on the instantaneous silent crop plant finds that the leaf rust resistance of the instantaneous silent crop plant is remarkably enhanced, and the leaf rust resistance of the instantaneous silent crop plant is remarkably improved. Furthermore, the application of the RNA interference fragment of the targeted Pt15401 gene and related biological materials in the aspect of improving the leaf rust resistance of the crops is proved, and gene resources and technical support are provided for subsequent breeding of the leaf rust resistant crops.

The invention provides a nucleic acid molecule for coding wheat stripe rust effect protein Pst13248 and application of the nucleic acid molecule, and belongs to the technical field of biological agriculture. The invention provides a nucleic acid molecule for coding wheat stripe rust effect protein Pst13248. According to the invention, Pst13248 of puccinia striiformis is silenced by an RNAi transgenic technology to obtain a Pst13248 silenced wheat material, and a wheat plant with wheat stripe rust resistance is obtained. In an infection experiment of stripe rust CYR32 on wheat, compared with a contrast Fielder, the Pst13248-RNAi wheat plant has the advantage that the Pst13248-RNAi wheat plant shows obvious resistance. Specifically, Pst13248-RNAi is a macroscopic phenotype that a large area of allergic necrosis reaction is generated at a leaf infected part of a wheat plant and uredospores of puccinia striiformis are reduced; the active oxygen area is increased, the cell necrosis area is increased, and strip rust hypha infection is blocked.

The invention provides a 2-substituted azole cell necrosis pathway inhibitor as well as a preparation method and application thereof. Specifically, the invention provides a compound with a structure as shown in a formula I, and each group is defined in the specification. The compound disclosed by the invention has very good inhibitory activity on RIPK3 and a cell necrosis pathway mediated by the RIPK3 at an enzyme level and a cell level. Therefore, the compound provided by the invention has a wide application prospect in the field of treatment or prevention of programmed cell necrosis and inflammation related diseases.

The invention discloses application of difluoromethyl ornithine in preparation of anti-tumor drugs, relates to the technical field of biological medicines, and has the technical key points that the difluoromethyl ornithine originally used for treating African faint sleep can effectively reduce the activity of various common tumors and rare tumor organs, reduce the tumor volume and improve the tumor activity. The obvious anti-tumor effect is shown; experimental data show that difluoromethyl ornithine shows high killing rate or cell necrosis rate in different tumor organs, and a new thought and an effective means are provided for treatment of malignant tumors.