56 results about "Cell necrosis" patented technology

The invention discloses an application of a compound in preparation of a product for enhancing the metabolic activity of hematopoietic stem cells and treating metachromatic white matter dystrophy. The compound comprises Azoramide. Azoramide is adopted to treat transfected hematopoietic stem cells, and cell apoptosis, cell necrosis and mitochondrial respiration conditions of the hematopoietic stem cells are obviously improved, so that the hematopoietic stem cells are expected to be applied to enhancement of transfection efficiency and metabolic activity of the hematopoietic stem cells transfected by the ARSA gene and reduction of ROS generation, and the effectiveness of the hematopoietic stem cells in treatment of MLD diseases is enhanced.

The present invention relates to a compound of Formula 1, a preparation method thereof, a pharmaceutical composition comprising the same as an active ingredient, and a use thereof. The compound of Formula 1 according to the present invention can exhibit cell necrosis and ferroptosis inhibitory efficacy in various cells such as heart, kidney, nerve, retina, liver, or lung cells, and accordingly, can be usefully used for prevention or treatment of cell necrosis or ferroptosis-related diseases.

The present invention relates to a compound of Formula 1, a preparation method thereof, a pharmaceutical composition comprising the same as an active ingredient, and a use thereof. The compound of Formula 1 according to the present invention can exhibit cell necrosis and ferroptosis inhibitory efficacy in various cells such as heart, kidney, nerve, retina, liver, or lung cells, and accordingly, can be usefully used for prevention or treatment of cell necrosis or ferroptosis-related diseases

The utility model relates to the technical field of beauty instruments, in particular to an ultrasonic anti-aging system, which comprises an ultrasonic transducer array module, an ultrasonic transducer array module, an ultrasonic transducer array module, an ultrasonic transducer array module, an ultrasonic transducer array module, an ultrasonic transducer array module, an ultrasonic transducer array module and an ultrasonic transducer array module, the switch module is used for switching among different modules; the processing circuit module is used for controlling and monitoring the whole treatment process; and the display module is used for providing real-time visual feedback and displaying imaging results of the skin and the subcutaneous tissue and parameters and effects in the treatment process. According to the utility model, high-frequency sound waves pass through skin and other tissues, energy is focused to a specific position in a body, and the temperature of a focusing point is rapidly increased and can generally reach 65-85 DEG C, so that protein denaturation and cell necrosis of target tissues are caused, and surrounding tissues are hardly influenced.

The invention discloses an electric shock simulation training detection and evaluation method, system and applet, and relates to the technical field of electric shock simulation training, and the method comprises the following steps: selecting an electrical stimulation instrument, applying a preset controllable electrical stimulation signal to a designated muscle area, and simulating an electrical signal of a real external environment under a precise control condition; the electric field change of muscular tissues in a delimited area is directly monitored through a micro field effect sensor, and the diffusion trend of charges is reflected in real time; electric field change data obtained in real time is preprocessed by applying a signal processing technology, then key features reflecting charge accumulation are extracted, and deep analysis is performed on the extracted key features. The electric field change in the muscular tissue is monitored in real time, the charge accumulation risk is recognized in advance through the deep learning model, the pulse duty ratio is automatically adjusted when the risk occurs, abnormal polarization and electrolytic reaction are effectively prevented, and the risks of tissue damage, cell necrosis and nerve damage are reduced.

This invention discloses a virulent strain of type I canine adenovirus and its application in constructing animal infection models, belonging to the field of biotechnology. The virulent strain of type I canine adenovirus is named canine adenovirus type 1 strain 0601, with accession number CCTCC NO: V202604. The virus titer reaches 10. 7.5 TCID 50 / mL. This virus can be used to construct an animal model of canine adenovirus type I infection, which exhibits typical clinical symptoms, such as hepatic hemorrhage, hepatocellular necrosis, and venous and sinusoidal congestion. The constructed animal model can be used for future canine adenovirus vaccine development, immune protection assessment, and efficacy evaluation of preventive and therapeutic drugs, providing excellent biological materials and theoretical basis for further control of canine adenovirus type I.

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.

The invention belongs to the technical field of biological medicine and molecular biology, and particularly relates to application of a transcription factor ZHX2 in diagnosis and treatment of hepatic ischemia-reperfusion injury. Specifically, research finds that the expression of ZHX2 after ischemia reperfusion injury is reduced, and HIRI cell necrosis and generation and development of HIRI are obviously promoted by specifically knocking out Zhx2 from liver cells, which are further proved in marginal liver IRI, while the ZHX2 has a protective effect on marginal fatty liver ischemia reperfusion injury. In conclusion, the ZHX2 can be used as a biological marker and a related treatment target of the hepatic ischemia reperfusion injury, especially marginal hepatic ischemia reperfusion injury, so that the ZHX2 has good clinical application value and social benefit.

The present invention provides a 2-substituted azole cell necrosis pathway inhibitor, a preparation method therefor and a use thereof. Specifically, the present invention provides a compound having a structure represented by formula I, each group being as defined in the description. The compound of the present invention has strong inhibitory activity against RIPK3 and a RIPK3-mediated cell necrosis pathway at both enzyme level and cell level. Therefore, the compound provided by the present invention has broad application prospects in the field of treatment or prevention of programmed cell necrosis and inflammation-related diseases.

The invention discloses an application of an epoxidase type II inhibitor, the application is the application in preparation of drugs for treating liver injury, hepatitis, hepatic fibrosis, or liver function decline or complications thereof, and the application discovers that the existing COX-2 selective inhibitors such as celecoxib and the like and a novel efficient COX-2 selective inhibitor 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, the 2, 8-deuterated dimethyl-2-(trifluoromethyl)-2H-benzopyran-3-carboxylic acid disclosed by the invention has the effects of remarkably relieving liver injury, reducing necrosis focus and inflammatory cell infiltration of liver cells and resisting cholestatic hepatic fibrosis; in addition, hepatocyte lipid accumulation and ballooning induced by high fat diet can be reduced, and the progress of lipid hepatitis is remarkably inhibited; it is found for the first time that the traditional Chinese medicine composition can significantly reduce the concentration of serum bilirubin and play a role in relieving hyperbilirubinemia caused by liver injury or liver insufficiency; the traditional Chinese medicine composition can be used for treating neonatal jaundice, congenital bile duct atresia, cholestatic cirrhosis, acute toxic hepatic failure, bilirubin increase caused by acute attack of chronic hepatitis, chronic hepatic failure or hepatic encephalopathy and the like; therefore, the method has obvious economic benefit and clinical application value.

The application belongs to the technical field of biological medicine, and particularly relates to application of a compound Merulin A in preparation of an anti-glioma drug. The application provides application of the compound Merulin A in preparation of the anti-glioma drug. Experiments prove that the compound Merulin A exhibits significant anti-glioma activity, can effectively promote early apoptosis and cell necrosis of glioma cells, and further inhibit proliferation and migration of the glioma cells. Moreover, due to advantages of wide source, simple extraction process, short cycle and low cost, the compound Merulin A has a wide application prospect as the anti-glioma drug.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

The invention relates to the technical field of cell culture, discloses an integral bubble-eliminating organ chip, and aims to solve the problems of cell necrosis and experiment failure caused by bubbles in a perfusion process of an existing organ chip. The chip comprises a sealing cover plate, a substrate, a bubble trapping mechanism and an attachment mechanism, bubbles in perfusate are efficiently eliminated through the bubble trapping mechanism, the attachment mechanism provides a stable attachment surface for cells, bidirectional circulation of the perfusate is achieved, and a guide-in opening and a guide-out opening do not need to be distinguished. The bubble elimination rate reaches 99.9%, the cell survival rate is improved to 95% or above, the repeatability of experimental data is improved by 80%, expensive peripherals are not needed, the use threshold of an organ chip platform is lowered, and the method is suitable for long-term and high-throughput cell culture and organ function simulation experiments.

The invention provides preparation and application of a kidney dual-targeting black phosphorus nano platform for treating acute kidney injury. Firstly, black phosphorus nanosheets and NH2-PEG-NH2 are mixed and then dispersed into deionized water, centrifugation is repeated, supernate is discarded, and a BP-PEG solution is obtained; kim-1 targeted polypeptide LTH powder is dissolved in deionized water, then EDC and NHS are added for a light-shielding reaction, a BP-PEG solution is added, light-shielding stirring is continued to obtain a BP-PEG-LTH mixture, then 4-octyl itaconic acid is added, then a PBS solution is added, light-shielding magnetic stirring is carried out, and the kidney double-targeting black phosphorus nano platform is obtained. The kidney dual-targeting black phosphorus nano platform is used as an intravenous injection for treating acute kidney injury, oxidative stress can be rapidly and effectively inhibited, inflammation is resisted, intercellular burial is promoted, malignant development of AKI is inhibited to a great extent, and the purpose of short-term specific intervention treatment after AKI is achieved.

The invention provides a programmed cell necrosis inhibitor as well as a preparation method and application thereof. Specifically, the invention provides a compound, or pharmaceutically acceptable salts, hydrates or solvates thereof, and the compound is as shown in a formula I in the specification. The invention also provides a preparation method of the compound and a pharmaceutical composition containing the compound or application of the compound in treatment or prevention of programmed necrosis of cells and / or human receptor interacting protein 1 kinase (RIPK1) related diseases or symptoms. # imgabs0 #

This invention relates to a formulation and method of application of a multicomponent mycotoxin detoxifier for poultry feed, particularly female broiler chickens, which aims to provide protection to the Bursa Fabricius due to exposure to mycotoxins in the form of aflatoxin and ochratoxin. The mycotoxin detoxifier formulation according to this invention consists of an inorganic mycotoxin binder in the form of bentonite and / or zeolite, an organic mycotoxin binder in the form of pectin and / or esterified cellulose, and a natural antioxidant in the form of vitamin E and / or antioxidant herbal extracts. The formulation is applied to poultry feed at a dose of 2 to 2.5 g per kg of feed and is able to bind aflatoxin and ochratoxin simultaneously in the digestive tract, thereby reducing the bioavailability of mycotoxins.The application of this formulation has been proven to reduce histopathological damage to the Bursa of Fabricius, including lymphoid follicle depletion, cyst formation, cell necrosis, inflammatory cell infiltration, and tissue fibrosis, while also supporting the immune system function of female broiler chickens. This invention provides an effective, efficient, and easily applicable technical solution in the poultry farming industry to address mixed mycotoxin contamination in feed.

The implantable device disclosed by the invention is provided with at least one corrodible zinc-containing part, and the zinc content in the at least one zinc-containing part is within the range of [30,000, 3,000, 3,000]. The zinc content range of at least one of the zinc-containing parts is [50, 70] wt.%, the zinc in the zinc-containing parts is an amorphous structure, or the zinc content range of at least one of the zinc-containing parts is [50, 70] wt.%, and the microstructure of the zinc in the zinc-containing parts is selected from at least one of an amorphous structure, a non-equiaxed structure, an ultra-fine structure or an equiaxed structure with the grain size range of 7-14 grades, or the microstructure of the zinc in the zinc-containing parts is selected from at least one of an amorphous structure, a non-equiaxed structure, an ultra-fine structure or an equiaxed structure with the grain size range of 7-14 grades. The zinc content range of at least one zinc-containing part is (70, 100) wt.%, and the microstructure of zinc in the zinc-containing part is selected from at least one of a non-equiaxed crystal structure, an ultra-fine crystal structure or an equiaxed crystal structure with the grain size range of 7-14 grades. After the implantable instrument is implanted into a body, a zinc corrosion product is generated, and the concentration of the zinc corrosion product in the surrounding tissue of the zinc-containing part of the implantable instrument can inhibit smooth muscle cell proliferation and cannot cause normal tissue cell necrosis.

The invention discloses a liver single-cell necrosis intelligent identification method and system based on deep learning and a storage medium, and the method comprises the steps: obtaining and segmenting a WSI labeling file obtained after labeling a single-cell necrosis lesion in a liver WSI image, and obtaining Tile and Mask files; generating a training set and a test set; constructing a ResUNet model based on an encoder-decoder symmetric architecture, and training the ResUNet model to obtain an identification model; a weight based on a positive pixel ratio is introduced in the training process and is used for amplifying positive sample loss; evaluating and verifying the identification model by using the test set to obtain a trained identification model; and packaging and deploying the trained recognition model, performing intelligent recognition on the input WSI image, and outputting a single-cell necrosis lesion recognition result. By means of the method, interactive instant reasoning feedback and result visualization can be achieved, and an efficient, accurate and standardized technical solution is provided for toxicity pathology assessment.

The application belongs to the technical field of genetic engineering and molecular breeding, and particularly relates to application of TaHPPD protein in regulation of wheat stripe rust resistance. Moro The TaHPPD protein can weaken active oxygen (ROS) accumulation and cell necrosis reaction triggered by CNL class protein NLR, and reduce ion leakage. The application obtains TaHPPD knockout (TaHPPD-KO) wheat material by knocking out TaHPPD gene in wheat through gene editing technology, and obtains wheat with wheat stripe rust resistance. The results of the embodiment show that, compared with the control Fielder, the TaHPPD-KO wheat plant shows significant resistance. The TaHPPD protein can be used to regulate wheat stripe rust resistance, and can be used for cultivating wheat resistant to stripe rust.

The present invention discloses a metal complex tailed with a TDO inhibitor, and its preparation method and application. The preparation method of the metal complex tailed with a TDO inhibitor of the present invention is specifically as follows: in a light-proof and inert gas environment, a metal dimer precursor and a dechlorinating agent are added to anhydrous methanol, refluxed and stirred at 60°C for 0.2 to 72 hours, and filtered after the reaction; an immune checkpoint TDO inhibitor ligand is added to the filtrate, and the reaction is carried out in an anaerobic environment. After the reaction is complete, an anion salt is added thereto, the solution is filtered and concentrated, and then frozen. After freezing, centrifugation is performed to obtain a crude product, which is purified by column chromatography to obtain a metal complex. The aryl / cyclopentadienyl metal complex of the present invention can be well positioned in the nucleus of cancer cells, induce cell necrosis, lead to immunogenic cell death, and inhibit the expression of immune checkpoint TDO, thereby achieving good anticancer activity, ICD induction activity and immune checkpoint TDO inhibition activity.

The invention discloses a traditional Chinese medicine compound composition for preventing and treating liver injury as well as a preparation method and application thereof. The compound composition comprises verbena, alfalfa, Chinese ladiestresses root, hairyvein agrimony, fingered citron, malt and hyperforin perforatum. The traditional Chinese medicine composition is simple in composition and reasonable in proportion, and animal experiments prove that the traditional Chinese medicine composition can improve the liver function, relieve liver inflammation injury, inhibit liver cell necrosis and fatty degeneration, inhibit oxidative stress and resist lipid peroxidation injury and has good prevention and treatment effects on liver injury, cholestatic liver diseases and the like caused by drugs. And the traditional Chinese medicine composition can obviously improve the symptoms such as weakness, nausea, skin and sclera yellow staining, yellow urine, itching, stomachache, hypochondrium lumps, body emaciation and the like in the liver injury period. The medicine is simple in preparation method, convenient to take, low in price, small in side effect and good in curative effect, and provides a good choice for vast patients with hepatopathy.

This paper describes a class of dyes containing 1,4-diazabicyclo[2.2.2]octane, their preparation methods, and applications, belonging to the fields of optical diagnostics and biomedicine. The dye comprises the dye molecule and 1,4-diazabicyclo[2.2.2]octane covalently coupled to the dye. 1,4-diazabicyclo[2.2.2]octane directly quenches singlet oxygen generated by the dye, affecting the triplet state of the dye, thereby improving its anti-bleaching ability and photostability, and reducing phototoxicity during fluorescence imaging. Furthermore, the introduction of 1,4-diazabicyclo[2.2.2]octane significantly improves the brightness of the dye, inhibits photoscintillation, and enhances its water solubility. Its protonation characteristic enhances the dye's mitochondrial targeting ability. These compounds, by reducing singlet oxygen and enhancing the local photothermal and reactive oxygen species production in mitochondria, induce potent cell necrosis, apoptosis, and immunogenic cell death, comprehensively improving anticancer efficacy. These compounds exhibit excellent photostability and low skin toxicity, showing broad application prospects.

The present invention discloses a method and system for detecting and evaluating electroshock simulation training, relating to the technical field of electroshock simulation training, and comprising the following steps: selecting an electrostimulation instrument, applying a preset controllable electrostimulation signal to a specified muscle area, and simulating the electrical signal of a real external environment under precise control conditions; directly monitoring the electric field change of muscle tissue within a demarcated area through a micro field effect sensor to reflect the diffusion trend of charges in real time; applying signal processing technology to preprocess the electric field change data obtained in real time, then extracting key features reflecting charge accumulation, and deeply analyzing the extracted key features. By monitoring the electric field change in muscle tissue in real time, the present invention uses a deep learning model to identify the risk of charge accumulation in advance, and automatically adjusts the pulse duty ratio when the risk appears, effectively preventing abnormal polarization and electrolytic reactions, and reducing the risks of tissue damage, cell necrosis and nerve damage.

This invention discloses the disease resistance function and application of the CC domain of CNL protein 2541 involved in wheat stripe rust resistance, belonging to the field of functional gene technology. This invention provides the application of the 2541-CC gene domain in regulating wheat stripe rust resistance, and provides a recombinant expression vector overexpressing the 2541-CC gene domain, as well as a method for constructing plant germplasm overexpressing the 2541-CC gene domain using the recombinant expression vector. This invention found that plants overexpressing the 2541-CC gene domain exhibited significant resistance, increased leaf hypersensitive necrosis, reduced stripe rust urediniospores, increased reactive oxygen species, increased cell necrosis, and inhibited stripe rust growth and development, thus confirming that the 2541-CC gene domain is a key domain in the 2541 gene's disease resistance signal transduction.

The invention discloses a polypeptide for inhibiting RIPK1 protein liquid-liquid phase separation and application of the polypeptide in regulating and controlling programmed necrosis of cells, the polypeptide for inhibiting RIPK1 protein liquid-liquid phase separation is a fusion polypeptide of a cell penetrating peptide and an RIPK1 protein SUMO modification inhibition polypeptide, and the RIPK1 protein SUMO modification inhibition polypeptide is a targeting polypeptide targeting RIPK1 protein. The SUMO modification of lysine at the 305th site of the RIPK1 protein is inhibited. The polypeptide for inhibiting liquid-liquid phase separation of the RIPK1 protein can inhibit SUMOylation modification of endogenous RIPK1 protein in cells, so that liquid-liquid phase separation of the RIPK1 protein is limited, phase conversion of the RIPK1 protein is effectively relieved, and programmed cell necrosis mediated by the RIPK1 is remarkably reduced under the condition that the activity of RIPK1 kinase is not changed.

The embodiment of the invention provides a fitting method and device for an ablation field of an electrode needle, and belongs to the technical field of composite pulsed electric field tumor ablation. The fitting method comprises the following steps: acquiring input sample data of a target electrode needle, the input sample data being a three-channel image in a target area including a spatial area where the target electrode needle is located, the three-channel image is formed by splicing the following three cross section images with the same size: a CT image, a cross section image of an ablation field rough model of the target electrode needle on a cross section where the CT image is located, and a needle passage mask image of the cross section where the CT image is located; and inputting the input sample data of the target electrode needle into the network structure model to obtain a fitting ablation field of the target electrode needle. According to the fitting method, the ablation field form and the electric field intensity distribution under the action of the target electrode needle can be predicted, so that the size and the shape of the cell necrosis area under the action of the composite pulse electric field are predicted, and reference is provided for clinical treatment. The method has the advantages of high precision, high calculation speed and the like.

This invention belongs to the field of genetic engineering technology and discloses the application of the transcription factor TaNAC67 in regulating wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown in SEQ ID NO: 1. This transcription factor can induce cell necrosis and plays a positive regulatory role in the interaction between wheat and stripe rust fungus. The gene encoding this transcription factor... TaNAC67 This is a disease resistance-related gene that is strongly induced by stripe rust infection in the incompatible interaction between plants and stripe rust fungi. Its nucleotide sequence is shown in SEQ ID NO: 2. Based on these characteristics, this invention further obtains an overexpressing gene. TaNAC67 The transgenic plants were tested and found to exhibit resistance to stripe rust. This invention provides genetic resources and technical support for the subsequent genetic improvement of wheat materials resistant to stripe rust.

The invention discloses an application of AngII in preparation of a medicine for improving myocardial remodeling, and 50-100nM AngII can promote myocardial cells to proliferate and hypertrophy; the AngII of 100-1000 nM induces apoptosis or ferroptosis of myocardial cells, but the phenomenon of cell necrosis does not exist. It is proved for the first time that AngII has dose-effect difference, a small dose of AngII can promote proliferation and hypertrophy of myocardial cells, a large dose of AngII can cause apoptosis and ferroptosis of the myocardial cells, but the phenomenon of cell necrosis does not exist; for the first time, it is found that different pathological changes of myocardial cells can be induced when AngII with different concentrations is given to different areas of the myocardium, so that the myocardium is reconstructed according to design requirements, the heart structure is improved, the expectation of improving the heart function is achieved, and a new treatment strategy is provided for improving myocardial reconstruction.

The application discloses a liver single-cell necrosis intelligent identification method and system based on deep learning and a storage medium. The method comprises the following steps: acquiring a WSI annotation file after single-cell necrosis lesions in a liver WSI image are annotated and cutting, acquiring Tile and Mask files; generating a training set and a test set; constructing a ResUNet model based on an encoder-decoder symmetric architecture and training to obtain an identification model; introducing a weight based on a positive pixel ratio in the training process, which is used to amplify the positive sample loss; after the test set is used to evaluate and verify the identification model, a trained identification model is obtained; after the trained identification model is packaged and deployed, the input WSI image is intelligently identified, and a single-cell necrosis lesion identification result is output. Through the application, interactive instant inference feedback and visual results can be realized, and an efficient, accurate and standardized technical solution is provided for toxicity pathological evaluation.

Provided in the present invention are inhibitors for programmed cell necrosis, a preparation method therefor and a use thereof. Specifically, provided in the present invention are a compound represented by formula I and a composition comprising same. The described compound may be used to prepare a pharmaceutical composition for the prevention and / or treatment of diseases involving cell death and / or inflammation.