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High-glucose-tolerance crypthecodinium cohnii obtained from orient domestication, and preparation method and application thereof

A technology of Cryptidium kouli and high glucose, which is applied in the biological field to achieve the effect of increasing oil accumulation

Inactive Publication Date: 2017-04-26
KUNMING ZAONENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports of obtaining highly glucose-tolerant Cryptodinium algal strains using directed acclimatization

Method used

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  • High-glucose-tolerance crypthecodinium cohnii obtained from orient domestication, and preparation method and application thereof
  • High-glucose-tolerance crypthecodinium cohnii obtained from orient domestication, and preparation method and application thereof
  • High-glucose-tolerance crypthecodinium cohnii obtained from orient domestication, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Carry out the directional domestication of high-concentration glucose tolerance to Crypthecodinium cohnii (Crypthecodinium cohnii) ATCC 30556, comprising the following steps:

[0034] 1. Cultivate Cryptidinium in a medium containing glucose 9g / L, yeast extract powder 2g / L, and sea salt 25g / L, and control the initial inoculation OD 490 0.08, measured once every 12h, each group made 3 parallel samples, measured the absorbance value at a wavelength of 490nm with a Shimadzu UV-1750 spectrophotometer, after culturing for 36h, the OD 490 Can reach or exceed 0.8, draw a histogram, and then subculture; only the glucose concentration in the subculture medium is as follows figure 1 As shown, it gradually increases; Tianjin Uno Instrument Co., Ltd. HNY-202B shaker parameters are set to 180rpm, temperature 25 ℃;

[0035] 2. It is basically the same as step 1, except that the subculture time is 10 days, and the glucose concentration in the subculture medium is increased to 22g / L. U...

Embodiment 2

[0052] The procedure is the same as in Example 1, except that the glucose concentration is gradually increased by 4 g / L, and the subculture time is 200 days. Compared with the starting strain, the glucose tolerance of the obtained domesticated strain is significantly improved.

Embodiment 3

[0054] The procedure is the same as in Example 1, except that the subculture time is 400 days, and the glucose tolerance of the obtained domesticated strain is significantly improved compared with the starting strain.

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Abstract

The invention discloses a high-glucose-tolerance crypthecodinium cohnii domesticated strain, and a preparation method and an application thereof. The high-glucose-tolerance crypthecodinium cohnii strain is purchased from American Type Culture Collection ATCC, an algal strain with the code of ATCC 30556 is used as a starting strain, and crypthecodinium cohnii selects the strain from the ATCC (having the strain code of ATCC 30556) as the starting strain; the initial crypthecodinium cohnii ATCC 30556 is used as a processing object, and domestication culture is performed for multiple times in a by+ culture medium having glucose gradually added, and a processing object of each-time domestication culture is an algal strain obtained after last-time domestication culture; the glucose concentration is increased to 54 g / L after domestication is finished; the domestication process is performed totally for 650 days, and the total number of generations is 130; the obtained high-glucose-tolerance crypthecodinium cohnii domesticated strain is named as ALE3. The high-glucose-tolerance crypthecodinium cohnii domesticated strain has obvious economic advantages and industry prospects in fermentation production of docosahexaenoic acid (DHA).

Description

technical field [0001] The invention discloses a highly glucose-tolerant domesticated strain of Cryptidium koii, a preparation method and application thereof. Belongs to the field of biotechnology. Background technique [0002] Docosahexaenoic acid (DHA), which belongs to the omega-3 unsaturated fatty acid, is an extremely important fatty acid with high nutritional value, which plays a role in the normal development of the infant brain and the normal function of the adult brain. important role. DHA is traditionally extracted from deep-sea fish oil, but with the gradual reduction of fishery resources in the world, efforts to find other alternative sources of DHA have also been paid more and more attention. Among them, the use of heterotrophic metabolic fermentation of marine microalgae to produce DHA has been regarded as a major alternative. [0003] Cryptidium koirii has a good ability to synthesize DHA, and DHA accounts for more than 30% of cell lipids. DHA produced by ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/36C12P7/64C12R1/89
CPCC12N1/12C12N1/36C12P7/6463C12P7/6472C12N1/125C12R2001/89
Inventor 刘璐陈磊李兴锐张卫文
Owner KUNMING ZAONENG BIOTECH CO LTD
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