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Rhizomucor variabilis and its cultivation method, bacterial agent and application

A cultivation method and root hair technology, applied in the field of microorganisms, can solve problems such as short interval time, high multiple cropping index, incomplete straw decomposition, etc., and achieve high economic and social benefits, and rapid decay effects

Active Publication Date: 2019-09-03
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, my country's multi-cropping index is relatively high, and the interval between crops in each season is short. There is not enough time for soil microorganisms to decompose the straw returned to the field, resulting in incomplete decomposition of the straw in the soil after returning to the field, and crop residues affect the quality of sowing and seedling emergence. rate, crop growth and farming operations

Method used

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  • Rhizomucor variabilis and its cultivation method, bacterial agent and application
  • Rhizomucor variabilis and its cultivation method, bacterial agent and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0057] This preparation example is used to prepare the fungal seed liquid used in the following examples.

[0058] The above-mentioned fungal liquid culture medium was sterilized at 121°C for 15 minutes, and then cooled to room temperature. The bacterium liquid of the Rhizomucor variabilis strain of 3 volume % is inserted in the above-mentioned sterilized fungal liquid culture medium of 200mL, at 150-240rpm, cultivate 5 days under the culture condition of 25-35 ℃, so that its spore quantity reaches 10 7 cells / mL to obtain the fungal seed solution.

Embodiment 1

[0060] This example is used to illustrate the degradation ability of Rhizomucor versicolor of the present invention to cellulose.

[0061] Test group: Add filter paper strips (1g in weight) to 100mL inorganic salt liquid medium in the Erlenmeyer flask, autoclave at 121°C for 15min, cool to room temperature, add 1mL of the fungal seed solution prepared in the above preparation example , and then cultured at 170rpm and 30°C for 5 days.

[0062] Blank control group: except that 1 mL of fungal seed liquid was replaced by 1 mL of fungal liquid culture medium, other operating conditions were the same as those of the test group.

[0063] Wherein, the composition of the inorganic salt liquid medium is 1.5% by weight of NH 4 NO 3 , 1.0 wt% KH 2 PO 4 , 1.0 wt% K 2 HPO 4 , 0.05 wt% MgSO 4 , pH 6.0-7.5.

[0064] Dry the cultures of the test group and the blank control group together with the Erlenmeyer flasks, and then weigh them respectively to obtain the difference between the w...

Embodiment 2

[0067] This example is used to illustrate the ability of Rhizomucor versicolor of the present invention to degrade lignin in water.

[0068] Test group: Take 100mL sodium lignosulfonate inorganic salt liquid medium, autoclave at 121°C for 15 minutes, cool to room temperature, insert 1mL of the fungal seed liquid prepared in the above preparation example, and then sterilize at 170rpm and 30°C cultured for 5 days.

[0069] Blank control group: except that 1 mL of fungal seed liquid was replaced by 1 mL of fungal liquid culture medium, other operating conditions were the same as those of the test group.

[0070] Take out a little of the five-day cultured liquid of the test group and the blank control group, centrifuge at 12,000 rpm for 10 min, and get the supernatant. The absorbance of the supernatant was measured at 280 nm by a UV spectrophotometer. Wherein, the absorbance value of the test group was 0.140, and the absorbance value of the blank control group was 0.697.

[007...

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Abstract

The present invention relates to the field of microorganisms, and specifically discloses a strain of Rhizomucor variabilis, wherein the preservation number is CGMCC No.11114. The present invention further discloses a culture method for the Rhizomucor variabilis, and a bacterial agent containing the Rhizomucor variabilis. The invention further discloses applications of the Rhizomucor variabilis and the bacterial agent in straw degradation. According to the present invention, with the application of the Rhizomucor variabilis to degrade the straws in soil and water, the straws can rapidly rot and be degraded, nitrogen, potassium and other nutrition elements required by crop growth can be released, a series of problems that the slow straw degradation affects the succeeding crop rooting, the nutrient competition between microorganisms used during the degradation and crops can cause the nitrogen hunger of crops, and the like can be solved, and the high economic and social benefits are provided.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Rhizomucor versicolor, in particular to a strain of Rhizomucor versicolor with the function of degrading stalks, and the method for cultivating the Rhizomucor versicolor contains the Rhizomucor versicolor as an active ingredient Bacteria and their application in degrading straw. Background technique [0002] my country is a big agricultural country with abundant crop straw resources. According to statistics, my country produces 600-800 million tons of straw annually, of which 42% is returned to the field directly or through the abdomen, 30% is used as agricultural fuel, 8% is used for industrial or other purposes, and 20% is not used. In recent years, with the development of the rural economy and the improvement of farmers' living standards, straw is no longer used as the main fuel, and the amount of remaining straw has increased significantly. In summer and autumn, in o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34B09C1/10C12R1/645
Inventor 林森任立伟过钰梁王培红
Owner 粮华生物科技(北京)有限公司
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