Gene marker for screening pre-job entry of lead exposure worker, and application thereof

Abstract

The invention discloses a gene marker for screening the pre-job entry of a lead exposure worker. A biomarker is an XPO5 gene 3'UTR area rs2257082 polymorphic site; the XPO5 gene 3'UTR area rs2257082 polymorphic site is as follows: TGGTCCATCTGGCCTTCCAGATATA[C / T] GAGGCACTGGTGAGTGGGGAAGGCA. TC and CC genetypes in the XPO5 gene 3'UTR area rs2257082 polymorphic site of a testee are detected so as to judge whether the testee belongs to a lead-susceptible high risk group. According to the gene marker provided by the invention, DNA (Deoxyribose Nucleic Acid) is extracted from the blood of lead-endurance workers and the blood of lead-sensitive workers with matched ages, genders and the like for the first time, a plurality of sites which participate in miRNA (Micro Ribonucleic Acid) nuclear export mature access relevant genes are detected, and a situation that the mononucleotide polymorphism of XPO5 gene 3'UTR area rs2257082 polymorphic site and the lead susceptibility have dependency is finally verified so as to serve as the gene marker for the pre-job entry of the lead exposure worker. The gene marker has the advantages of simpleness in operation and high sensitivity and is suitable for screening susceptible population on a large scale, and a basis is provided for the pre-job entry of the lead exposure worker.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the application of DNA in blood cells, and discloses a biomarker for pre-employment screening of lead-exposed workers and an application thereof. Background technique [0002] Lead is ubiquitous because it is non-degradable in the environment. It can enter the body through the digestive tract, respiratory tract and other ways to affect the functions of multiple systems such as nerves, digestion, and hematopoiesis, causing damage to the body. (Muntner, P., 2, Menke, A., DeSalvo, K.B., 2, Rabito, F.A. & Batuman, V 2005) There is no threshold for neurodevelopmental toxicity caused by lead. Some studies have reported that even if the blood lead level is lower than 100μg / L There was neurodevelopmental toxicity (Akesson, A et al 2005). Therefore, searching for susceptibility markers of lead poisoning is helpful for early detection of lead poisoning susceptible populations. In previous studies,...

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Problems solved by technology

[0004] Although the pathogenic factors of lead poisoning have been relatively clear, in the same environment and at the same exposure level, individuals respond differently to lead, and there are great differences in individual blood lead concentrations, which suggests that our individual response to lead is not the same. Susceptibility differences exist with exposure

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