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Bifidobacterium capable of enhancing pancreatic function and application of bifidobacterium

An animal bifidobacteria, functional technology, applied in the directions of bifidobacteria, applications, and food ingredient functions, can solve the problems of monopoly of core technology and unclear mechanism of action, and achieve the improvement of the degree of vacuolar degeneration and the increase of the number of pancreatic islets Effect

Active Publication Date: 2017-05-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies have found that probiotics have good biological effects on improving type 2 diabetes, but their mechanism of action has not been clarified, and many functional probiotic strains, fermentation agents and core technologies are monopolized by foreign countries

Method used

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  • Bifidobacterium capable of enhancing pancreatic function and application of bifidobacterium
  • Bifidobacterium capable of enhancing pancreatic function and application of bifidobacterium
  • Bifidobacterium capable of enhancing pancreatic function and application of bifidobacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1, the preparation of bifidobacterium A6 bacterial suspension

[0084] 1. Inoculate the activated Bifidobacterium A6 into the MRS liquid medium, culture anaerobically at 37°C for 12 hours, and centrifuge the culture system at 4000g for 15 minutes to collect the bacteria. The cells were washed twice with PBS, and then the cells were resuspended with normal saline to obtain the A6 live cell suspension.

[0085] 2. Inoculate the activated Bifidobacterium A6 into the MRS liquid medium, culture anaerobically at 37°C for 12 hours, and centrifuge the culture system at 4000g for 15 minutes to collect the bacteria. The cells were washed twice with PBS, and then the cells were resuspended with RPMI-1640 complete medium to obtain A6 viable cell suspension.

[0086] 3. Inoculate the activated Bifidobacterium A6 into the MRS liquid medium, culture anaerobically at 37°C for 12 hours, put the culture system in a water bath at 72°C for 10 minutes after the cultivation, and ...

Embodiment 2、II

[0088] Embodiment 2, establishment of type II diabetes rat model and animal experiments

[0089] Experimental rats: 60 healthy male SD rats (240±10g) aged 6-8 weeks. Rats were housed in stainless steel dry-housed experimental cages to prevent coprophagia. The temperature of the animal experiment room was maintained at 22±2°C, the humidity was maintained at 55%±5%, and the 12h light / 12h dark cycle was strictly followed.

[0090] 1. The experimental rats were randomly divided into two groups (normal group and model group), 10 in the normal group and 50 in the model group. The rats in the normal group were fed with basic diet, and the rats in the model group were fed with high-fat diet for 4 weeks (week 1-4).

[0091] 2. After completing step 1, all rats were fasted and fed for 12 hours without water intake, and then the body weight of the rats was measured. The rats in the normal group were intraperitoneally injected with 0.1mmol / L citric acid buffer solution with a pH value o...

Embodiment 3

[0110] Example 3, Detection of the effect of Bifidobacterium A6 on RIN-m5F of β-insulinoma cells

[0111] 1. Preparation of macrophages

[0112] Male SD rats were sacrificed by vertebral dislocation, followed by the following operations:

[0113] (1) Soak the rat in 75% (volume percent) ethanol water solution for 30s, and put it on the ultra-clean table with the abdomen facing upward.

[0114] (2) Carefully cut off the abdominal fur to expose the peritoneum, and inject 10 mL of pre-cooled PBS buffer solution containing 3% (volume percentage) fetal bovine serum into the abdominal cavity with a sterile syringe.

[0115] (3) Gently rub the abdomen, suck out the peritoneal fluid with a syringe, and place it in a clean centrifuge tube.

[0116] (4) The peritoneal fluid was centrifuged at 300 g for 5 min to collect the cells, washed twice with 37° C. preheated PBS buffer, and resuspended with RPMI-1640 complete medium.

[0117] (5) Counting with a hand-held cell counter, accordin...

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Abstract

The invention discloses bifidobacterium capable of enhancing the pancreatic function and application of the bifidobacterium. The invention discloses application of animal bifidobacterium in preparation of a product for preventing and / or treating diabetes. By research on the apparent characteristics of the bifidobacterium to type II diabetes and development of the state of the illness, the bifidobacterium has a delaying effect and other good biological effects, and a certain theoretical basis is supplied to relevant experimental researches on the diabetes.

Description

technical field [0001] The invention relates to a bifidobacterium for enhancing the function of pancreatic islets and its application. Background technique [0002] Diabetes (diabetes) is an endocrine and metabolic disease related to abnormal production and action of insulin and characterized by hyperglycemia, mainly including type I and type II diabetes. Type II diabetes is the main type of diabetes in my country, accounting for more than 90% of the total number of diabetic patients. Type II diabetes, which is mainly based on the relative lack of insulin and insulin resistance, will eventually lead to hyperglycemia, and long-term hyperglycemia symptoms will cause a series of chronic complications, such as foot disease (foot ulcers, infection and gangrene), nephropathy (kidney function) exhaustion, uremia), eye disease (retinopathy, blurring, blindness), encephalopathy (cerebrovascular disease), heart disease, skin disease, sexually transmitted disease, etc. Type II diabet...

Claims

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Application Information

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IPC IPC(8): A23L33/135A61K35/745A61P3/10
CPCA61K35/745A23V2002/00A23V2400/51A23V2200/328
Inventor 任发政姜南燕赵亮杨子彪
Owner CHINA AGRI UNIV
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