Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

PF-127-miRNA-615 agomir complex and preparation method and application thereof

A technology of pf-127-mirna-615agomir and 1.pf-127-mirna-615agomir is applied in the field of PF-127-miRNA-615agomir complex and its preparation, and achieves the effects of high disability rate and poor prognosis

Active Publication Date: 2017-05-10
GUANGDONG MEDICAL UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the intracellular mechanism of its function is still unknown

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PF-127-miRNA-615 agomir complex and preparation method and application thereof
  • PF-127-miRNA-615 agomir complex and preparation method and application thereof
  • PF-127-miRNA-615 agomir complex and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Preparation of PF-127-miRNA-615 agomir complex

[0035] The PF-127-miRNA-615 agomir complex was prepared from Pluronic F-127 hydrogel, deionized water and miRNA-615 agomir by the following steps:

[0036] (1) Synthesis of miRNA-615 agomir:

[0037] In this example, miRNA-615 agomir is a miRNA-615 analog synthesized by special chemical modification, which was synthesized by Guangzhou Ruibo Biotechnology Co., Ltd.;

[0038] The basic information of miRNA-615 is shown in Table 1:

[0039] Table 1 Basic information of miRNA-615

[0040]

[0041]

[0042] The reaction mechanism between miRNA-615 and LINGO-1: Mature miRNA mainly affects the post-transcriptional translation efficiency and stability of genes by binding to the 3'-UTR of specific target genes. Mature miRNA-615 through its seed sequence (seedsequence)─ GGGGGUCCCC (Table 1 single underlined part) specifically binds to the partial sequence of the 3'-UTR of LINGO-1 mRNA (Table 1 double underli...

Embodiment 2

[0051] Example 2: Application of PF-127 encapsulating miRNA-615 agomir complex to repair brachial plexus root avulsion

[0052] 1. Preparation of rat model with brachial plexus root avulsion

[0053] Adult female SD rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (3.5ml / kg). Under a stereomicroscope, the right C4-T2 spinal cord segments were exposed, C4-C7 laminectomy was performed, and the right arm was separated. For the C5-C7 spinal nerve roots of the plexus, use a fine glass hook to gently tear off the C5-C7 nerve roots, and cut off a section of the spinal nerve connected to the C5 and C7 nerve roots, leaving a gap of about 5mm between the nerve and the spinal cord, and then remove the The anterior root of C6 was reset back to its original position, the bleeding was fully stopped, and the muscles and skin were sutured layer by layer intermittently. Keep warm after the operation, and send the animal to a clean animal breeding room for routine car...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biomedicine, in particular to PF-127-miRNA-615 agomir complex and a preparation method and application thereof, wherein the PF-127-miRNA-615 agomir complex is made by covering miRNA-615 agomir with Pluronic F-127 hydrogel. A transporter capable of carrying miRNA-615 agomir and filling crack damage is provided, precise transport and local slow release of miRNA-615 agomir are achieved, and three-dimensional space and mechanical supporting capacity can be provided for the growth of neural axons. Therefore, the PF-127-miRNA-615 agomir complex can vigorously promote nerve regenerative repair for brachial plexus root avulsion, and new treatment routes are provided for central and peripheral nerve injury.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a PF-127-miRNA-615agomir complex and its preparation method and application. Background technique [0002] Brachial plexus avulsion (BPA) is a common disabling surgical trauma, especially total brachial plexus root avulsion, with high disability rate, poor curative effect and poor prognosis. With the continuous development of family motor vehicles and modern transportation and logistics, the incidence of brachial plexus injuries has increased year by year, and the most common causes are tractive injuries caused by traffic accidents and neonatal dystocia. The nerve root is composed of a central part and a peripheral part, and the junction part of the two is called the "transitional zone" (transitional zone, TZ). Brachial plexus avulsion is the physical separation of the nerve root and the spinal cord in the TZ region, which not only causes peripheral nerve rupture, but also l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K48/00A61K31/7105A61K9/06A61K47/34A61P25/02A61L27/54A61L27/18A61L27/52
CPCA61K9/06A61K31/7105A61K47/34A61L27/18A61L27/52A61L27/54A61L2300/258A61L2300/60A61L2430/32C08L71/08
Inventor 吴洪福丁璐周光纪曾丽妮朱哲刘晓倩罗传铭邹堂斌孙雪荣邱文锋张文辉崔新月钟家贵
Owner GUANGDONG MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com