30 results about "Peripheral nerve damage" patented technology

A method of enhancing the regeneration of injured nerves has the step of administering an effective exposure of pressure pulses or acoustic shock waves in a pulse or wave pattern to the zone of injury of the nerve during the regeneration process. The inventive method may include enhancing the stimulation of neuronal cell growth or regeneration by administering an effective exposure of pressure pulses or acoustic shock waves in a pulse or wave pattern to stimulate neuronal cell growth or regeneration, wherein the administering of the treatment is applied to a patient who has a pathological condition where neuronal repair can be facilitated including peripheral nerve damage caused by injury or disease such as diabetes, brain damage associated with stroke, and for the treatment of neurological disorders related to neurodegeneration, including Parkinson's disease, Alzheimer's disease and amyotrophic lateral, sclerosis multiple sclerosis and disseminated sclerosis. The treatment is ideally suited for neural regeneration after a degenerative condition due to any neurological infections or any other pathological condition.

The present invention provides a peripheral nerve growth conduit for peripheral nerve repair, in particular conduits through which peripheral nerves can grow. The conduit includes poly-ε-caprolactone (PCL). Preferably, the inner (luminal) surface of the conduit comprises pits having a depth of 1-4 μm. Suitably, the conduit may also include poly-lactic acid (PLA). The inner surface of the conduit may have been treated with an alkaline composition. The present invention also provides a method for treating a peripheral nerve damage using a peripheral nerve growth conduit including poly-ε-caprolactone (PCL). The present invention also provides a kit for treating a peripheral nerve damage having a peripheral nerve growth conduit including poly-ε-caprolactone (PCL).

Belonging to the technical field of medical biomaterials, the invention in particular relates to a polylactic acid-chitosan composite nerve conduit. The composite nerve conduit is composed of oriented polylactic acid electrospun fiber and a chitosan layer wrapping the fiber. The invention also provides a preparation method of the polylactic acid / chitosan composite nerve conduit, and application of the nerve conduit in preparation of peripheral nerve defect repair materials. The polylactic acid-chitosan composite nerve conduit prepared by the invention has excellent biomechanical properties and superior hydrophilcity, also has good biological tissue compatibility, can significantly promote the attachment, migration and proliferation of Schwann cells, is easy to cut and preserve, and is simple to operate during clinical use, thus having good clinical application prospects in the peripheral nerve damage repair field.

The present invention relates to the field of biomedicine, and specifically relates to a method for culturing and purifying perineurial cells in vitro. The steps are as follows: (a) take the sciatic nerve of SD rats, separate and obtain the perineurium, and culture it as a patch to obtain mixed growth nerves Perinecular cells, fibroblasts and Schwann cells; (b) first remove Schwann cells by time-limited digestion, and then initially remove fibroblasts by differential adhesion method; (c) repeat the above operation once; (d) Fibroblasts were further removed with the chemical drug cytarabine. The invention establishes a fast and efficient method for purifying and culturing perineurial cells in vitro, and provides a stable cell source for in-depth research on the mechanism of peripheral nerve injury repair.

The present invention belongs to the field of gene engineering medicine, in particular, its relates to a method for producing and preparing human brain-derived neurotrophic factor (BDNF) by utilizing engineering bacterium pichia pastoris and its application in preparation of medicine for promoting development, growth and differentiation of several sensory neurons of peripheral neural crest and basal plate source, maintaining nerve center basal forebrain, cholinergic neuron, GABA neuron and mesencephalic substantic nigra dopaminergic neuron and curing some neurogenic disease, such as progerine dementia, parkinsonism and injury and peripheral nerves.

The invention provides a cell-free biological material of Acarina platicum, a preparation method and an application thereof, and relates to the technical field of biomedical materials. The biological material includes the extracellular matrix material formed by decellularization of the graminosaur, which well retains the original circular muscle and longitudinal muscle structure of the graminus, and well retains the orientation of the muscle bundles The aligned nanofibrous structure, which can promote the penetration, adhesion and growth of cells, can be used in wound care and / or to promote the healing and repair of other tissues. The invention also provides a preparation method of the biological material and its application in the fields of peripheral nerve injury repair and dermal tissue regeneration.

The invention provides benzothiazole compounds having a structure represented by the formula (I). The provided compounds have an excellent protective effect on nerves, can be used to treat diseases related with nerve damages, such as facial nerve damage, peripheral nerve damage caused by body damage or diseases (diabetes, eg.), neurological disorders (Parkinson's disease, Alzheimer disease, amyotrophic lateral sclerosis, etc.) caused by neural degeneration, and the like, and is preferentially used to treat facial nerve damage.

The invention discloses a slow release diaphragm and making method and application to do local usage of immune inhibitor FK506 to accelerate the regeneration of nerve, which is characterized by the following: the slow release diaphragm can be copolymer (PLGA) of decomposably biological macromolecular material-polylactic acid (PLA) [2] and polyglycolic acid (PGA), which is composed of immune inhibitor FK506 and slow release carrier. The making method of the slow release diaphragm comprises the following steps: a. reacting polylactic acid (PLA) [2] and polyglycolic acid (PGA) to produce the copolymer PLGA; b. blending the copolymer PLGA and FK506; adding the mixture into deionized water to dilute; removing organic solvent; stirring under indoor temperature to evaporate the solvent; freezing; drying; obtaining nanometer microball; c. using the nanometer microball into drug diaphragm to make local exterior drug to restore the peripheral nerve damage.

The invention discloses a preparation method of a drug-loaded nanofiber nerve guide, which comprises the following steps: (1) using chitosan and astragalus polysaccharide as raw materials, sodium tripolyphosphate as a crosslinking agent, and preparing astragalus by a chemical crosslinking method polysaccharide nanospheres; (2) mixing astragalus polysaccharide nanospheres with gelatin solution, and then vacuum freeze-drying to obtain the inner core of the nerve conduit; (3) dissolving gelatin and polylactic acid in hexafluoroisopropanol, and configuring it into a spinning solution, using the electrospinning method to prepare the fiber nerve guide shell; (4) filling the inner core of the nerve guide in the cavity of the fiber nerve guide shell, and then adding it to the genipin solution for cross-linking, after washing Freeze-dried, that is. The nerve conduit prepared by the method has excellent biocompatibility, mechanical properties and degradation properties, can provide porous channels for nerve fiber regeneration, and can slowly release astragalus polysaccharides to further accelerate the nerve repair process, thereby better treating peripheral nerve injuries.

The invention discloses a method for preparing a collagen material for repairing peripheral nerve injury. The method comprises the following step: co-incubating a ciliary neurotrophic factor with a collagen binding domain (CBD-CNTF), a basic fibroblast growth factor with a collagen binding domain (CBD-bFGF) and an orderly collagen material to obtain a collagen material for repairing the peripheral nerve injury. According to the preparation method, two factors CBD-CNTF and CBD-bFGF are simultaneously crosslinked to the orderly collagen scaffold material, and the action of the double-factor peripheral nerve injury repairing collagen material in long-cross-section nerve injury repairing can be evaluated by establishing an animal peripheral nerve-facial nerve long cross-section injury model. Results prove that compared with single factor CBD-CNTF or CBD-bFGF functional collagen scaffold material, the double-factor peripheral nerve injury repairing collagen material can be used for better promoting regeneration of facial nerves and restoration of functions, and has a more important realistic guiding magnificence to application of clinical peripheral nerve injury repairing in the future.