30 results about "Peripheral nerve damage" patented technology

A method of enhancing the regeneration of injured nerves has the step of administering an effective exposure of pressure pulses or acoustic shock waves in a pulse or wave pattern to the zone of injury of the nerve during the regeneration process. The inventive method may include enhancing the stimulation of neuronal cell growth or regeneration by administering an effective exposure of pressure pulses or acoustic shock waves in a pulse or wave pattern to stimulate neuronal cell growth or regeneration, wherein the administering of the treatment is applied to a patient who has a pathological condition where neuronal repair can be facilitated including peripheral nerve damage caused by injury or disease such as diabetes, brain damage associated with stroke, and for the treatment of neurological disorders related to neurodegeneration, including Parkinson's disease, Alzheimer's disease and amyotrophic lateral, sclerosis multiple sclerosis and disseminated sclerosis. The treatment is ideally suited for neural regeneration after a degenerative condition due to any neurological infections or any other pathological condition.

The invention relates to a preparation method of a controlled-release oriented nanofiber nerve conduit. The method comprises the following steps: preparing a core layer solution, preparing a skin layer solution, performing coaxial electrospinning, preparing a controlled-release oriented nanofiber membrane with a high-speed rotary drum receiving device and preparing the nanofiber nerve conduit. The preparation method in the invention has advantages of simple and practical, rich raw material sources, good biocompatibility and biodegradability, and is easy to realize industrial production. The prepared nerve conduit has wide application prospects in the clinical treatment of peripheral nerve damage.

The invention belongs to the fields of biomedical materials and nerve tissue engineering, and relates to a preparation method of a composite nano-fiber nerve scaffold suitable for peripheral nerve regeneration. The composite nano-fiber scaffold with conductivity and a function of promoting regeneration by traditional Chinese medicines is prepared by taking a biochemical macromolecular / conductive material with biocompatibility as shell pre-electrospinning liquid, an extracellular matrix component or an extracellular matrix component / traditional Chinese medicine extract as a core pre-electrospinning liquid and by an electrostatic spinning technology. The composite nano-fiber scaffold with electric stimulation and traditional Chinese medicines, prepared according to the method, can control effective release of various medicines, is high in biocompatibility, stable in performance and easy to store, and has good application prospect in the fields of biomedicine such as peripheral nerve damage repair. The method is simple in operation, the process is time-saving, the raw materials are moderate in price and easily available, the reaction condition is mild, and the method is environmentally friendly, high in economic performance and suitable for industrial production.

Belonging to the technical field of medical biomaterials, the invention in particular relates to a polylactic acid-chitosan composite nerve conduit. The composite nerve conduit is composed of oriented polylactic acid electrospun fiber and a chitosan layer wrapping the fiber. The invention also provides a preparation method of the polylactic acid / chitosan composite nerve conduit, and application of the nerve conduit in preparation of peripheral nerve defect repair materials. The polylactic acid-chitosan composite nerve conduit prepared by the invention has excellent biomechanical properties and superior hydrophilcity, also has good biological tissue compatibility, can significantly promote the attachment, migration and proliferation of Schwann cells, is easy to cut and preserve, and is simple to operate during clinical use, thus having good clinical application prospects in the peripheral nerve damage repair field.

The invention discloses silk fibroins with different degradation rates and a use thereof. Natural silk fibers are treated by lithium bromide, calcium chloride or acid-base to form silk fibroins degraded at a slow rate, a middle rate and a fast rate, and the silk fibroins are freeze-dried and molded and then is subjected to alcohol treatment so that silk fibroin supports with different degradation rates are obtained. Under different hydrolysis conditions, a silk fibroin degradation rate can be effectively controlled, and in nerve injury restoration, a nerve revegetation rate is equal to a support material degradation rate. A research proves that the silk fibroin supports with fast and medium degradation rates can promote nerve damage restoration and has good curative effects on peripheral nerve damage treatment.

The invention discloses a method for preparing a collagen material used for repairing peripheral nerve injury. The preparation method provided by the invention comprises the following steps: co-incubating vascular endothelial growth factors (VEGF) with collagen binding domains and an ordered collagen material, so as to obtain the collagen material used for repairing peripheral nerve injury. Experiment results show that the collagen material containing VEGF-CBD factors and used for repairing peripheral nerve injury can promote peripheral nerve regeneration, as well as exponential recovery of impaired peripheral nerve functions, electrophysiological recovery of peripheral nerve transaction parts, expression of S-100 proteins and / or neurofilament proteins of peripheral nerve injured parts, increase of diameter and / or wall thickness of myelin sheaths of the peripheral nerve injured parts and recovery of target organs, which proves that the functional material has more practical guiding significance on future clinical application to peripheral nerve injury repairing.

Provided herein are methods of treating a peripheral nerve injury in a subject. The methods include administering to the subject at or near the site of the peripheral nerve injury an effective amount of a composition comprising an agent that promotes remyelination of the peripheral nerve. Also provided are methods of determining whether a peripheral nerve injury has a capacity for recovery. The methods include selecting a subject with a peripheral nerve injury, administering to the subject a first dose of a composition comprising and agent that promote remyelination and detecting after the first dose one or more characteristics of peripheral nerve recovery, the presence of one or more characteristics of peripheral nerve recovery indicating a peripheral nerve injury has a capacity for recovery and the absence of characteristics of peripheral nerve recovery indicating a peripheral nerve injury without a capacity for recovery.

The present invention provides a peripheral nerve growth conduit for peripheral nerve repair, in particular conduits through which peripheral nerves can grow. The conduit includes poly-ε-caprolactone (PCL). Preferably, the inner (luminal) surface of the conduit comprises pits having a depth of 1-4 μm. Suitably, the conduit may also include poly-lactic acid (PLA). The inner surface of the conduit may have been treated with an alkaline composition. The present invention also provides a method for treating a peripheral nerve damage using a peripheral nerve growth conduit including poly-ε-caprolactone (PCL). The present invention also provides a kit for treating a peripheral nerve damage having a peripheral nerve growth conduit including poly-ε-caprolactone (PCL).

The invention provides a method for detecting the expression quantity of HOXD9 after peripheral nerve injury. The method comprises the following steps: (1) separating dorsal root ganglion neuron nucleoproteins at different time points after sciatic nerve injury of a rat, performing transcription factor activity detection, and determining the expression quantity change trend of a transcription factor HOXD9 in the nerve regeneration process; (2) observing the expression quantity and the expression trend of related messenger RNA results of HOXD9 by applying a PCR technology; (3) displaying intracellular localization of the HOXD9 in an DRG neuron by adopting an immunofluorescence staining technology; and (4) counting the HOXD9 fluorescence intensity change trends at different time points afterthe sciatic nerve injury, and enabling the fluorescence intensity to be the highest on the third day and to be consistent with a result trend obtained by a PCR experiment. The expression quantity change of HOXD9 after peripheral nerve injury is clarified, and the regulation effect of THE HOXD9 on neuron axon regeneration is discovered.

The present invention provides a peripheral nerve growth conduit for peripheral nerve repair, in particular conduits through which peripheral nerves can grow. The conduit includes poly-ε-caprolactone (PCL). Preferably, the inner (luminal) surface of the conduit comprises pits having a depth of 1-4 μm. Suitably, the conduit may also include poly-lactic acid (PLA). The inner surface of the conduit may have been treated with an alkaline composition. The present invention also provides a method for treating a peripheral nerve damage using a peripheral nerve growth conduit including poly-ε-caprolactone (PCL). The present invention also provides a kit for treating a peripheral nerve damage having a peripheral nerve growth conduit including poly-ε-caprolactone (PCL).

The invention discloses a directional fibrin and polypeptide interpenetrating network composite hydrogel for nerve regeneration and a preparation method thereof, and belongs to the technical field ofbiomedical materials. The composite hydrogel is composed of a fibrin and polypeptide interpenetrating composite nanofiber structure. The directional fibrin hydrogel is prepared by electrostatic spinning, cross-linked polypeptide is interpenetrated with fibrin through swelling and physical adsorption, and then is stably combined with fibrin through a variety of cross-linking methods to form the directional interpenetrating network composite hydrogel. The composite hydrogel is a composite hydrogel with high water content, has the composition, structure and mechanical properties highly simulatingthe extracellular matrix of natural nerve tissues, contains polypeptide fragments simulating the function of growth factors, has excellent effects of promoting nerve cell adhesion and nerve fiber directional growth and inducing nerve and blood vessel regeneration, and can be applied to nerve regeneration repair of brain, spinal cord and peripheral nerve injury.

Belonging to the technical field of medical biomaterials, the invention in particular relates to a polylactic acid-chitosan composite nerve conduit. The composite nerve conduit is composed of oriented polylactic acid electrospun fiber and a chitosan layer wrapping the fiber. The invention also provides a preparation method of the polylactic acid / chitosan composite nerve conduit, and application of the nerve conduit in preparation of peripheral nerve defect repair materials. The polylactic acid-chitosan composite nerve conduit prepared by the invention has excellent biomechanical properties and superior hydrophilcity, also has good biological tissue compatibility, can significantly promote the attachment, migration and proliferation of Schwann cells, is easy to cut and preserve, and is simple to operate during clinical use, thus having good clinical application prospects in the peripheral nerve damage repair field.

The invention relates to the technical field of biomedicine, in particular to PF-127-miRNA-615 agomir complex and a preparation method and application thereof, wherein the PF-127-miRNA-615 agomir complex is made by covering miRNA-615 agomir with Pluronic F-127 hydrogel. A transporter capable of carrying miRNA-615 agomir and filling crack damage is provided, precise transport and local slow release of miRNA-615 agomir are achieved, and three-dimensional space and mechanical supporting capacity can be provided for the growth of neural axons. Therefore, the PF-127-miRNA-615 agomir complex can vigorously promote nerve regenerative repair for brachial plexus root avulsion, and new treatment routes are provided for central and peripheral nerve injury.

The invention discloses a pichia yeast recombinant bacterial strain highly expressing a Neuritin truncated protein, and an application thereof. The recombination bacterial strain is Pichia pastoris and has a preservation number of CGMCC NO:9912. Compared with Neuritin truncated proteins expressed by Escherichia coli and sold in the present market, the Neuritin truncated protein expressed by the pichia yeast recombinant bacterial strain has the following advantages: an exogenous protein expressed by eukaryotic cells has post-translational modification bioactivity, and exogenous gene is stably integrated in yeast chromosome, and can realize stable passage and easy large scale culture. A suitable concentration of Neuritin can promote the regeneration and function recovery of early stage sciatic nerves after injuries, provides a new method for treatment of peripheral neuropathy injury, and has good application prospect.

The present invention relates to an application of easy-taking peripheral nervous half-implantation type electrical stimulation system. The system is composed of functional output device, functional input device, data output device, information output device, electric power input device and contact device. The invention comprises applications of said system in peripheral nervous injured diseases, including: peripheral nerve denervated caused by violence trauma such as cutting, puncturing, breaking or indirect injury such as fracture, dislocation; peripheral nerve avulsion caused by draw-off; peripheral nerve destroyed caused by crush; peripheral nerve incidental injury caused by operation and neural inflammatory caused by various factors. The system enlarges application range of peripheral nervous half-implantation type electrical stimulation system in medical field, and takes the implanted electrical stimulation guide wire out directly or by assistant instrument monitoring after the treating course for nerve injured patient is finished, reduces tedious operation of taking out again and improves safety and practicality.

The invention discloses an analgesic and antipruritic pharmaceutical composition and an application method thereof, and belongs to the technical field of medical chemistry. The research of the invention finds that the analgesic effect generated by independently inhibiting the activity of Nav1.7 or Nav1.8 is reduced along with the time history of peripheral nerve injury, and also finds that a neuropathic pain mouse with poor response to the Nav1.7 small-molecule inhibitor has better response to the Nav1.8 small-molecule inhibitor. According to the discovery, the analgesic effect of combined administration of different Nav1.7 inhibitors and Nav1.8 inhibitors is researched, and it is found that the analgesic effect can be remarkably improved and the response rate can be increased to 100% by combined administration of appropriate doses of the Nav1.7 inhibitor PF-05089771 and the Nav1.8 inhibitor PF-04885614. Therefore, the composition of the PF-05089771 and the PF-04885614 can be used for treating diseases caused by too high activity of the Nav1.7 or the Nav1.8, and the diseases include but are not limited to intractable pain and itching.

The present invention relates to the field of biomedicine, and specifically relates to a method for culturing and purifying perineurial cells in vitro. The steps are as follows: (a) take the sciatic nerve of SD rats, separate and obtain the perineurium, and culture it as a patch to obtain mixed growth nerves Perinecular cells, fibroblasts and Schwann cells; (b) first remove Schwann cells by time-limited digestion, and then initially remove fibroblasts by differential adhesion method; (c) repeat the above operation once; (d) Fibroblasts were further removed with the chemical drug cytarabine. The invention establishes a fast and efficient method for purifying and culturing perineurial cells in vitro, and provides a stable cell source for in-depth research on the mechanism of peripheral nerve injury repair.

Disclosed herein are biomimetic scaffolds for nervous tissue growth having a plurality of microchannels disposed within a sheath. Each microchannel includes a porous wall formed of a biocompatible and biodegradable material. The biocompatible and biodegradable material may be a poly (ethylene glycol) diacrylate, a methacrylated gelatin, a methacrylated collagen, or a polycaprolactone, and combinations thereof. The bionic stent has a high open volume percentage, can achieve excellent (linear and high fidelity) nervous tissue growth, and meanwhile reduces inflammation near an in-vivo implantation site to the maximum extent.

The invention provides benzothiazole compounds having a structure represented by the formula (I). The provided compounds have an excellent protective effect on nerves, can be used to treat diseases related with nerve damages, such as facial nerve damage, peripheral nerve damage caused by body damage or diseases (diabetes, eg.), neurological disorders (Parkinson's disease, Alzheimer disease, amyotrophic lateral sclerosis, etc.) caused by neural degeneration, and the like, and is preferentially used to treat facial nerve damage.

The invention relates to a neurotrophin sustained-release coacervate for curing peripheral nerve injury and its application thereof. The neurotrophin sustained-release coacervate includes polyethylene arginine aspartic acid glyceraldehyde (PEAD), glycosaminoglycans (GAGs) and neurotrophic factors (NTFs). By changing the different ratio of components and the type of NTFs, it can improve the performance of the sustained-release coacervate. The preparation process of the invention-neurotrophin sustained-release coacervate is simple, the drug-loading is large, the stability is good, which is easy to be degraded. This invention is safe and has no side effect, and it has remarkable long lasting sustained-release effect, so as to satisfy the requirements of biomaterials for repairing peripheral nerve injury ideally.

The invention provides a cell-free biological material of Acarina platicum, a preparation method and an application thereof, and relates to the technical field of biomedical materials. The biological material includes the extracellular matrix material formed by decellularization of the graminosaur, which well retains the original circular muscle and longitudinal muscle structure of the graminus, and well retains the orientation of the muscle bundles The aligned nanofibrous structure, which can promote the penetration, adhesion and growth of cells, can be used in wound care and / or to promote the healing and repair of other tissues. The invention also provides a preparation method of the biological material and its application in the fields of peripheral nerve injury repair and dermal tissue regeneration.

The invention provides benzothiazole compounds having a structure represented by the formula (I). The provided compounds have an excellent protective effect on nerves, can be used to treat diseases related with nerve damages, such as facial nerve damage, peripheral nerve damage caused by body damage or diseases (diabetes, eg.), neurological disorders (Parkinson's disease, Alzheimer disease, amyotrophic lateral sclerosis, etc.) caused by neural degeneration, and the like, and is preferentially used to treat facial nerve damage.

The invention discloses a slow release diaphragm and making method and application to do local usage of immune inhibitor FK506 to accelerate the regeneration of nerve, which is characterized by the following: the slow release diaphragm can be copolymer (PLGA) of decomposably biological macromolecular material-polylactic acid (PLA) [2] and polyglycolic acid (PGA), which is composed of immune inhibitor FK506 and slow release carrier. The making method of the slow release diaphragm comprises the following steps: a. reacting polylactic acid (PLA) [2] and polyglycolic acid (PGA) to produce the copolymer PLGA; b. blending the copolymer PLGA and FK506; adding the mixture into deionized water to dilute; removing organic solvent; stirring under indoor temperature to evaporate the solvent; freezing; drying; obtaining nanometer microball; c. using the nanometer microball into drug diaphragm to make local exterior drug to restore the peripheral nerve damage.

A biomaterial for the treatment of spinal cord or of peripheral nerve injury injury, obtainable by: a) treating a hyaluronic acid derivative with a coating solution promoting Neuronal Stem Cells adhesion, branching and differentiation; b) contacting isolated Neuronal Stem Cells with the hyaluronic acid derivative obtained from step a) and culturing and expanding the absorbed cells in the presence of growth or neurotrophic factors selected from βFGF (basic fibroblast growth factor), CNTF (ciliary neurotrophic factor), BDNF (brain derived neurotrophic factor) and GDNF (glial derived neurotrophic factor) or mixtures thereof.

The invention discloses a preparation method of a drug-loaded nanofiber nerve guide, which comprises the following steps: (1) using chitosan and astragalus polysaccharide as raw materials, sodium tripolyphosphate as a crosslinking agent, and preparing astragalus by a chemical crosslinking method polysaccharide nanospheres; (2) mixing astragalus polysaccharide nanospheres with gelatin solution, and then vacuum freeze-drying to obtain the inner core of the nerve conduit; (3) dissolving gelatin and polylactic acid in hexafluoroisopropanol, and configuring it into a spinning solution, using the electrospinning method to prepare the fiber nerve guide shell; (4) filling the inner core of the nerve guide in the cavity of the fiber nerve guide shell, and then adding it to the genipin solution for cross-linking, after washing Freeze-dried, that is. The nerve conduit prepared by the method has excellent biocompatibility, mechanical properties and degradation properties, can provide porous channels for nerve fiber regeneration, and can slowly release astragalus polysaccharides to further accelerate the nerve repair process, thereby better treating peripheral nerve injuries.

The invention provides an artificial nerve conduit with PLGA (poly (lactic-co-glycolic acid)) and acellular extracellular matrix as substrates, and a preparation method and device, and belongs to the technical field of biological materials or biological manufacturing or peripheral nerve repair or tissue engineering, and the preparation method comprises the following steps: preparing a nerve conduit; preparing a decellularized kidney; preparing decellularized nerves; preparing the decellularized muscle; and preparing the composite catheter containing the decellularized tissue. According to the embodiment of the invention, the artificial nerve conduit which is high enough in strength and has promoting significance on nerve regeneration can be prepared, meanwhile, the recovery time of a patient suffering from peripheral nerve injury can be shortened, the prognosis of the patient is further improved, and the life quality of the patient is improved.

The invention discloses a slow release diaphragm and making method and application to do local usage of immune inhibitor FK506 to accelerate the regeneration of nerve, which is characterized by the following: the slow release diaphragm can be copolymer (PLGA) of decomposably biological macromolecular material-polylactic acid (PLA) [2] and polyglycolic acid (PGA), which is composed of immune inhibitor FK506 and slow release carrier. The making method of the slow release diaphragm comprises the following steps: a. reacting polylactic acid (PLA) [2] and polyglycolic acid (PGA) to produce the copolymer PLGA; b. blending the copolymer PLGA and FK506; adding the mixture into deionized water to dilute; removing organic solvent; stirring under indoor temperature to evaporate the solvent; freezing; drying; obtaining nanometer microball; c. using the nanometer microball into drug diaphragm to make local exterior drug to restore the peripheral nerve damage.

The invention discloses a method for preparing a collagen material for repairing peripheral nerve injury. The method comprises the following step: co-incubating a ciliary neurotrophic factor with a collagen binding domain (CBD-CNTF), a basic fibroblast growth factor with a collagen binding domain (CBD-bFGF) and an orderly collagen material to obtain a collagen material for repairing the peripheral nerve injury. According to the preparation method, two factors CBD-CNTF and CBD-bFGF are simultaneously crosslinked to the orderly collagen scaffold material, and the action of the double-factor peripheral nerve injury repairing collagen material in long-cross-section nerve injury repairing can be evaluated by establishing an animal peripheral nerve-facial nerve long cross-section injury model. Results prove that compared with single factor CBD-CNTF or CBD-bFGF functional collagen scaffold material, the double-factor peripheral nerve injury repairing collagen material can be used for better promoting regeneration of facial nerves and restoration of functions, and has a more important realistic guiding magnificence to application of clinical peripheral nerve injury repairing in the future.

The invention discloses application of cytokine activin C in inhibiting neuropathic pain and application of the activin C in preparation medicines for treating neuropathic pain, and discloses a medicine for treating neuropathic pain. The medicine at least comprises activin C. The peripheral nerve injury induced neuropathic pain model prepared in the invention verifies that endogenous activin C isremarkably expressed and remarkably up-regulated in small neurons of dorsal root ganglion (DRG), and chronic neuropathic pain can be inhibited by adjusting TRPV1 channels if the activin C is applied in peripheral local and intrathecal parts, and the potential of the activin C for treating chronic neuropathic pain can be prompted.