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Functional biomaterial capable of guiding peripheral nerve regeneration and preparation method thereof

A technology for peripheral nerve injury and collagen material, applied in the field of medical materials, can solve problems such as side effects and infection, and achieve the effects of promoting recovery, promoting peripheral nerve regeneration, and promoting the increase in the diameter of the myelin sheath and/or the thickness of the myelin sheath wall.

Inactive Publication Date: 2016-05-11
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But such an approach can lead to infection or serious side effects

Method used

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  • Functional biomaterial capable of guiding peripheral nerve regeneration and preparation method thereof
  • Functional biomaterial capable of guiding peripheral nerve regeneration and preparation method thereof
  • Functional biomaterial capable of guiding peripheral nerve regeneration and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Preparation of Collagen Scaffold for Repairing Peripheral Nerve Injury

[0056] 1. Preparation of ordered collagen materials

[0057] Prepared by our laboratory, the method is as in the reference patent ZL200510098731.5, and the specific steps are as follows:

[0058] (1) Material collection and processing

[0059] 1. Pretreatment of fascia

[0060] Fresh adult bovine muscle with white fascia was taken and washed three times with cold deionized water; the fascia was separated with tweezers and a scalpel, and the inner layer of adhered muscle tissue and outer layer of fat and other tissues were removed as much as possible.

[0061] 2. Material preparation

[0062] The pretreated fascia obtained in step 1 is processed as follows:

[0063] 1) placed in 1% (volume percentage) TnBP (tributyl phosphate) solution (50mM Tris-HCl buffer, pH 8.0) for 48 hours;

[0064] 2) Treat in 50mM Tris-HCl buffer (pH8.0, containing 1MNaCl) for 48 hours;

[0065] 3) 1g (2.5×1...

Embodiment 2

[0095] Example 2. Application of Peripheral Nerve Injury Function Repair Collagen Scaffold to Treat Transverse Sciatic Nerve Injury in SD Rats

[0096] 1. Prepare the sciatic nerve transection injury model of SD rats, and bridge the peripheral nerve injury function repair collagen scaffold material at both ends of the nerve injury

[0097] 1. Experiment preparation

[0098] Purchase 60 adult male SD rats, weighing between 200 and 220 g, SD rats were purchased from Weitong Lihua Company. The consumables prepared before the operation are as follows: surgical needle and thread, sterile filter paper, mouse board, gel pen, watch, elbow scissors, round scissors, straight tweezers, straight scissors, ophthalmic scissors, ophthalmic tweezers, surgical blades, knife handles , hemostatic forceps, watch forceps, sterile hole towel, needle filter, disposable syringe, disposable bacterial dish, pentobarbital sodium, normal saline, PBS, 75% ethanol, small beaker, distilled water, garbage b...

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Abstract

The invention discloses a method for preparing a collagen material used for repairing peripheral nerve injury. The preparation method provided by the invention comprises the following steps: co-incubating vascular endothelial growth factors (VEGF) with collagen binding domains and an ordered collagen material, so as to obtain the collagen material used for repairing peripheral nerve injury. Experiment results show that the collagen material containing VEGF-CBD factors and used for repairing peripheral nerve injury can promote peripheral nerve regeneration, as well as exponential recovery of impaired peripheral nerve functions, electrophysiological recovery of peripheral nerve transaction parts, expression of S-100 proteins and / or neurofilament proteins of peripheral nerve injured parts, increase of diameter and / or wall thickness of myelin sheaths of the peripheral nerve injured parts and recovery of target organs, which proves that the functional material has more practical guiding significance on future clinical application to peripheral nerve injury repairing.

Description

technical field [0001] The invention belongs to the field of medical materials, and relates to a functional biomaterial guiding peripheral nerve regeneration and a preparation method thereof. Background technique [0002] Peripheral nerve injury (PNI) often results in a loss of patient-related function, affecting the patient's quality of life. Peripheral nerve injury caused by birth trauma, car accident or violent injury can lead to degeneration and necrosis of sensory or motor neurons. Despite the ability to regenerate peripheral nerves, functional recovery is far from ideal. The continuous development of artificial nerve research has effectively promoted the regeneration and functional recovery of nerve axons. Collagen material is one of the better materials in tissue engineering. Collagen materials are widely used in tissue regeneration due to their low immunogenicity and good degradability. [0003] Growth factors play an important role in promoting nerve growth in t...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/54
Inventor 戴建武赵燕南肖志峰陈冰
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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