Preparation method of composite nano-fiber nerve scaffold

A technology of composite nanofibers and nerves, applied in the field of biomedical materials and nerve tissue engineering, can solve the problems of charge instability, electric stimulation intensity and duration difficult to control, etc., achieve time-saving process, promote nerve regeneration and structural reconstruction , the effect of good application prospects

Inactive Publication Date: 2017-11-03
武汉康华世纪药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the surface charge of these materials is mainly due to the capture of charge carriers or small mechanical defor

Method used

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  • Preparation method of composite nano-fiber nerve scaffold
  • Preparation method of composite nano-fiber nerve scaffold
  • Preparation method of composite nano-fiber nerve scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Take 1.0 g of natural graphite powder and 0.5 g of sodium nitrate in a three-necked flask, slowly add 23 ml of concentrated sulfuric acid in an ice bath, and continue stirring for 15 min. Then slowly add 5g of potassium permanganate and keep the system temperature not exceeding 20°C, and continue to stir for 2h. The ice bath was removed and the system was heated to 35 °C, stirring was continued for 1.5 h. Add 46ml of deionized water slowly, maintain the system temperature at 98°C, and stir vigorously for 30min. Then cool in air, add 140ml deionized water and 10ml hydrogen peroxide to stop the reaction, the suspension turns bright yellow. Wash by centrifugation for 10 min, fully wash with 5% HCl and deionized water until the pH is close to neutral, and freeze-dry to obtain brown solid graphite oxide. Disperse the obtained solid into deionized water (1mg / ml), and ultrasonically exfoliate it with a power of 50W for 10min to obtain graphene oxide nanosheets, ce...

Embodiment 2

[0029]Example 2: Take 1.0 g of natural graphite powder and 0.5 g of sodium nitrate in a three-necked flask, slowly add 23 ml of concentrated sulfuric acid in an ice bath, and continue stirring for 15 min. Then slowly add 5g of potassium permanganate and keep the system temperature not exceeding 20°C, and continue to stir for 2h. The ice bath was removed and the system was heated to 35 °C, stirring was continued for 1.5 h. Add 46ml of deionized water slowly, maintain the system temperature at 98°C, and stir vigorously for 30min. Then cool in air, add 140ml deionized water and 10ml hydrogen peroxide to stop the reaction, the suspension turns bright yellow. Wash by centrifugation for 20 min, fully wash with 5% HCl and deionized water until the pH is close to neutral, and freeze-dry to obtain brown solid graphite oxide. Disperse the obtained solid into deionized water (1mg / ml), and ultrasonically exfoliate it with 100W power for 20min to obtain graphene oxide nanosheets, centrif...

Embodiment 3

[0030] Example 3: Take 1.0 g of natural graphite powder and 0.5 g of sodium nitrate in a three-necked flask, slowly add 23 ml of concentrated sulfuric acid in an ice bath, and continue stirring for 15 min. Then slowly add 5g of potassium permanganate and keep the system temperature not exceeding 20°C, and continue to stir for 2h. The ice bath was removed and the system was heated to 35 °C, stirring was continued for 1.5 h. Add 46ml of deionized water slowly, maintain the system temperature at 98°C, and stir vigorously for 30min. Then cool in air, add 140ml deionized water and 10ml hydrogen peroxide to stop the reaction, the suspension turns bright yellow. Wash by centrifugation, fully wash with 5% HCl and deionized water until the pH is close to neutral, and freeze-dry to obtain brown solid graphite oxide. Disperse the obtained solid into deionized water (1mg / ml), and ultrasonically exfoliate it with 180W power for 90min to obtain graphene oxide nanosheets, centrifuge for 30...

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Abstract

The invention belongs to the fields of biomedical materials and nerve tissue engineering, and relates to a preparation method of a composite nano-fiber nerve scaffold suitable for peripheral nerve regeneration. The composite nano-fiber scaffold with conductivity and a function of promoting regeneration by traditional Chinese medicines is prepared by taking a biochemical macromolecular/conductive material with biocompatibility as shell pre-electrospinning liquid, an extracellular matrix component or an extracellular matrix component/traditional Chinese medicine extract as a core pre-electrospinning liquid and by an electrostatic spinning technology. The composite nano-fiber scaffold with electric stimulation and traditional Chinese medicines, prepared according to the method, can control effective release of various medicines, is high in biocompatibility, stable in performance and easy to store, and has good application prospect in the fields of biomedicine such as peripheral nerve damage repair. The method is simple in operation, the process is time-saving, the raw materials are moderate in price and easily available, the reaction condition is mild, and the method is environmentally friendly, high in economic performance and suitable for industrial production.

Description

technical field [0001] The invention belongs to the fields of biomedical materials and nerve tissue engineering, and relates to a preparation method of a composite nanofiber nerve support suitable for regeneration of peripheral nerves. Background technique [0002] Peripheral nerve injuries are very common in clinical practice, especially the repair of long-distance nerve defects has always been a difficult problem in the medical field. So far, several processes such as nerve anastomosis, autologous nerve transplantation, allogeneic nerve transplantation, autologous non-nerve tissue transplantation and biomaterial replacement have been experienced, but each treatment has its own advantages and disadvantages, and the problem of nerve defect repair has not been fundamentally resolved. solution. [0003] In the face of long-distance nerve injury, the focus of current research is to process biomaterials into neural scaffolds with tubular structures to regenerate nerves. This s...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/56A61L27/58A61L27/20A61L27/18A61L27/16A61L27/08A61L27/04D01F1/09D01F1/10D01F8/10D01F8/14
CPCA61L27/047A61L27/08A61L27/16A61L27/18A61L27/20A61L27/3637A61L27/50A61L27/56A61L27/58A61L2300/30A61L2300/412A61L2300/604A61L2400/12A61L2430/32D01F1/09D01F1/10D01F8/10D01F8/14
Inventor 徐海星许沛虎黄吉林王晓冰布颖郭兴蕾郑芙蓉
Owner 武汉康华世纪药业有限公司
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