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A kind of acellular biomaterial, preparation method and application thereof

A technology of stellate worms and biological materials, which is applied in unknown raw materials, pharmaceutical formulations, tissue regeneration, etc., can solve the problems of lack of porous structure of acellular dermal matrix, insufficient precision and volume of processing technology, and poor wound healing effect. , to achieve the effect of solving the poor stability of the preparation technology, realizing the industrialization of the product and high safety

Active Publication Date: 2021-12-07
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims to solve the difficulties in the prior art of insufficient precision and volume in the processing technology of nerve-induced regenerative materials with ordered micro-nano topological structure, and difficulty in realizing industrialization. It is not conducive to vascularization, and the technical defect of promoting wound healing is not good

Method used

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  • A kind of acellular biomaterial, preparation method and application thereof
  • A kind of acellular biomaterial, preparation method and application thereof
  • A kind of acellular biomaterial, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation of embodiment 1 decellularized gridworm biological material

[0049] (1) Material collection: select fresh phalanx (naked phalanx), cut it open along the longitudinal muscle, wash the inner and outer body walls, and remove the coelom membrane.

[0050] (2) Disinfection and stabilization: The body wall of the processed Spina grisa was washed twice with sterile saline containing 50 mM ascorbic acid and 500 ppm streptomycin at 4° C. for 1 hour.

[0051] (3) Acid treatment: put the body wall of Spina gridia in 2% salicylic acid solution, and stir at 37° C. for 24 hours. Then soak and rinse with normal saline.

[0052] (4) Decellularization:

[0053] a) Put the cleaned body wall of P. grisatrix in 3% Triton-100, shake and soak at room temperature for 48 hours, during which the Triton-100 solution was replaced every 24 hours, and then rinsed with normal saline.

[0054] b) Treat with 2.5% (w / v) trypsin solution at 4°C for 10 hours. Then wash with saline. ...

Embodiment 2

[0061] The preparation of embodiment 2 decellularized gridworm biological material

[0062] (1) Material collection: Select fresh Starworm grid, cut along the longitudinal muscle, wash the inner and outer body walls, and remove the coelom membrane.

[0063] (2) Disinfection and stabilization: the body wall of the treated P. grisa was washed twice at 4° C. for 1 hour with sterile phosphate buffer solution containing 50 mM ascorbic acid and 500 ppm streptomycin respectively.

[0064] (3) Acid treatment: put the body wall of Spina gridia in 2% salicylic acid solution, and stir at 37° C. for 24 hours. Then soak and rinse with sterile phosphate buffered saline.

[0065] (4) Decellularization:

[0066] a) Place the washed Starworm body wall in 3% Triton-100, shake and soak at room temperature for 48 hours, during which the Triton-100 solution is replaced every 24 hours, and then use sterile phosphate buffer saline rinse.

[0067] b) Treat with 2.5% (w / v) trypsin solution at 4°C ...

Embodiment 3

[0073] The preparation of embodiment 3 decellularized gridworm biological material

[0074] (1) Material collection: Select fresh Starworm grid, cut along the longitudinal muscle, wash the inner and outer body walls, and remove the coelom membrane.

[0075] (2) Disinfection and stabilization: the body wall of the treated P. grisa was washed twice at 4° C. for 1 hour with sterile phosphate buffer solution containing 50 mM ascorbic acid and 500 ppm streptomycin respectively.

[0076] (3) Acid treatment: put the body wall of Scutellaria gridia in 2% acetic acid solution, and stir at 37° C. for 24 hours.

[0077] (4) Separation: spread the acid-treated material, manually separate the transverse muscle and longitudinal muscle of the body wall of A. platyphagia, and then soak and rinse with sterile phosphate buffer saline.

[0078] (5) Decellularization: a) Place the washed A. platyphagia muscle bundles in 3% Triton-100, shake and soak at room temperature for 48 hours, during which...

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Abstract

The invention provides a cell-free biological material of Acarina platicum, a preparation method and an application thereof, and relates to the technical field of biomedical materials. The biological material includes the extracellular matrix material formed by decellularization of the graminosaur, which well retains the original circular muscle and longitudinal muscle structure of the graminus, and well retains the orientation of the muscle bundles The aligned nanofibrous structure, which can promote the penetration, adhesion and growth of cells, can be used in wound care and / or to promote the healing and repair of other tissues. The invention also provides a preparation method of the biological material and its application in the fields of peripheral nerve injury repair and dermal tissue regeneration.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a cell-free biomaterial of Acarina graminis, a preparation method and an application thereof. Background technique [0002] Tissues and organs of human or animal origin can maintain their original tissue structure and biological activity after decellularization. Biomaterials obtained by decellularization technology have been widely used in the research of bone, cartilage, blood vessel, soft tissue, nerve and other tissue defect repair. With the continuous maturity of decellularization technology, the research on acellular matrix biomaterials has been deepened, and the safety and effectiveness of acellular matrix biomaterials have gradually been clinically verified. At present, the clinically used acellular matrix biomaterials are mainly allogeneic or xenogeneic tissues. Among them, allogeneic tissues still face a shortage of donors; xenogeneic tissues are mainly de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/56A61L27/58A61L27/60A61K35/655A61P25/02
CPCA61L27/3633A61L27/3675A61L27/3687A61L27/3691A61L27/50A61L27/56A61L27/58A61L27/60A61L2300/412A61L2430/32A61L2430/40A61K35/655A61P25/02
Inventor 代元坤李八方宋文山王园园
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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