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Silk fibroins with different degradation rates and use thereof

A technology of silk fibroin and degradation speed, applied in the field of biomedical materials, can solve the problems of nerve growth without support, complete repair, inflammatory response, etc., achieve non-toxic mechanical properties, promote nerve damage repair, and promote axis The effect of outgrowth

Inactive Publication Date: 2016-10-26
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the re-growth rate of nerves is slower than the degradation rate of silk fibroin, it will lead to the residue of foreign matter in the body, which will cause an inflammatory response and cause certain disease-associated symptoms to the human body; if the re-growth rate of nerves is faster than the degradation rate of silk fibroin, It will lead to later nerve growth without stent support, and the effect of complete repair cannot be achieved.

Method used

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  • Silk fibroins with different degradation rates and use thereof
  • Silk fibroins with different degradation rates and use thereof
  • Silk fibroins with different degradation rates and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of the natural silk fiber of desericin

[0032] The electronic balance weighs 40-50g of natural silk fiber wrapped with sericin, and puts it into a stainless steel container of about 5L. Then, take 10.18g of anhydrous sodium carbonate and pour it into a container, pour 2000ml of triple distilled water (about 0.5% sodium carbonate alkaline solution), stir it fully with a glass rod to make it completely dissolve, press the floating silk with a glass rod The fibers are mostly submerged in the sodium carbonate solution. Then, heat it on the induction cooker until it boils, stir the silk with a glass rod, start timing from boiling, cook for about 30 minutes, and stir it properly with a glass rod during this period to make the silk fiber cook evenly. After cooking, pour off the sodium carbonate solution and wash twice with triple distilled water to expose the silk fibroin and increase the whiteness. In order to fully remove the sericin, carry o...

Embodiment 2

[0033] Embodiment 2: the preparation of slowly degrading silk fibroin

[0034] Weigh 40.45g of lithium bromide and pour it into a 50ml centrifuge tube, add triple distilled water to make it up to 50ml, after fully dissolving, add it to a 100ml beaker under stirring conditions, and add 2.5g of Example 1 at a mass volume ratio of 1:20 to obtain The silk fibroin protein (adding in several times), after adding completely, hydrolyze and stir under the conditions in Table 1, and stand still at room temperature for 30min. Then, dialysis (molecular weight cut off: 12000-14000) was performed for 3 days.

[0035] Table 1

[0036] Group

Hydrolysis temperature

Hydrolysis time

1

25℃

360min

2

70℃

10min

3

50℃

185min

4

70℃

500min

[0037] SDS-PAGE gel electrophoresis was used to test the molecular weight of silk fibroin in groups 1-4. Using 12% SDS-PAGE separating gel (Milli-Q water, 2ml; 30% Acr-Bis (29:1), 4ml; 1M ...

Embodiment 3

[0039] Embodiment 3: the preparation of slowly degrading silk fibroin

[0040] Weigh 37g of anhydrous calcium chloride, put it into a 250ml beaker, add 48ml of triple distilled water to dissolve all the calcium chloride, and heat the water bath to 75-80°C. Then, add 38.78ml of absolute ethanol, add the silk fibroin protein prepared in Example 1 according to the mass volume ratio of 1:20 (adding in several times), continue to stir at this temperature for 10-60min after adding completely (Table 2), Then let cool at room temperature, and perform dialysis (molecular weight cut-off: 12000-14000) for 3 days.

[0041] Table 2

[0042] Group

Hydrolysis temperature

Hydrolysis time

1

80℃

10min

2

78℃

30min

3

75℃

60min

[0043] SDS-PAGE gel electrophoresis was used to test the molecular weight of the silk fibroin in groups 1-3. The test results showed that the hydrolyzed silk fibroin with calcium chloride had a molecular weigh...

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Abstract

The invention discloses silk fibroins with different degradation rates and a use thereof. Natural silk fibers are treated by lithium bromide, calcium chloride or acid-base to form silk fibroins degraded at a slow rate, a middle rate and a fast rate, and the silk fibroins are freeze-dried and molded and then is subjected to alcohol treatment so that silk fibroin supports with different degradation rates are obtained. Under different hydrolysis conditions, a silk fibroin degradation rate can be effectively controlled, and in nerve injury restoration, a nerve revegetation rate is equal to a support material degradation rate. A research proves that the silk fibroin supports with fast and medium degradation rates can promote nerve damage restoration and has good curative effects on peripheral nerve damage treatment.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and specifically relates to a biocompatible, biodegradable, and porous scaffold material based on biological macromolecules, which can be used as tissue engineering materials such as nerve injury repair, drug slow-release carriers, genes and high performance composites. Background technique [0002] Peripheral nervous system injuries caused by traffic accidents, violent blows, industrial production accidents, accidental injuries in life, and surgery are very common in clinical practice. After peripheral nerve injury, the corresponding neuron cell body undergoes a series of morphological, biochemical, metabolic and gene expression changes, which is the response of neuron cell body to axonal injury. According to the degree of axonal damage, the injury response of neuron cell body is different, and severe cases will cause the death of neuron cell body. Furthermore, inflammatory cells invade, gl...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/12A61L27/22A61L27/54A61L27/58
CPCA61L27/22C07K14/435C07K14/43586A61L27/227A61L27/54A61L27/58A61L2300/252A61L2300/412A61L2300/602A61L2300/604
Inventor 杨宇民张鲁中顾晓松丁斐李贵才赵亚红杨晓明
Owner NANTONG UNIVERSITY
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