76 results about "Alcohol treatment" patented technology

Modified mineral fillers, derived from either a coarse particle kaolin clay or a coarse particle ATH, having excellent utility in thermoset polymers as low paste viscosity fillers are disclosed. The method of treatment involves the use of specific weight ratio combinations of a silane and a long chain aliphatic alcohol. The treatment of clay can also involve the auxiliary use of a surfactant in small amounts, which is utilized to emulsify the silane and aliphatic alcohol treatment components in water either individually or as a co-emulsion blend prior to their addition to the clay. The surfactant, which is preferably a nonionic surfactant having a HLB value of 12-18, aides in uniformly treating all the clay particle surfaces with the silane / alcohol treatment combination. Coarse particle clays having general utility in the invention are those having an average Stokes equivalent particle diameter of at least 3.0 microns, while the coarse particle ATH fillers having general utility in the invention are those having a BET surface area value less than 5.0 m2 / g. Clays or ATH so treated with the inventive silane / alcohol treatment provide unique viscosity reduction benefits in thermoset polymers unattainable by using either treatment additive alone in any amount. This low viscosity characteristic allows the treated mineral products to be used at high loadings in various thermoset compounds, as fillers or extenders, which is advantageous in terms of reducing cost and / or gaining certain performance properties, such as physical reinforcement, composite surface smoothness or flame retardancy.

The provides a catalyst carrier for olefin polymerization reaction with preparation method and application. Wherein, using normal spray drying method to prepare spherical magnesium halide or magnesium halide-inorganic carrier; taking makeup-alcohol treatment, and obtaining the carrier. This method can generate multi-phase magnesium alkoxide with higher Mg content and catalysis activity, needs mild reaction condition, and decreases break of catalyst.

Disclosed are a rare earth permanent magnet and a preparation method. The preparation method of the rare earth permanent magnet is capable of improving coercivity of the magnet while keeping binding strength of a base body and a plated layer or coated layer after plating or coating on the basis without reducing remanence and magnetic energy product of the magnet and includes: firstly dissolving powder of fluoride containing R2, nitrate containing R3 and phosphate containing R4 into water or alcohol to prepare solution; uniformly applying the solution on the surface of a magnet to be treated; then dewatering or performing alcohol treatment on the magnet to be treated; finally, enabling the magnet to be treated to be subjected to diffusion heat treatment to obtain the treated magnet. By the aid of the preparation method, uniformity of the powder on the surface of the magnet can be improved, powder residues on the surface of the magnet after heat diffusion are reduced, the coercivity of the magnet is improved, and the binding strength of the magnet of the base body and the plated layer or coated layer after plating or coating is kept constant.

The present invention discloses one kind of cell culturing rack material and its preparation process. The cell culturing rack material is prepared with silk as main material, and through degumming, dissolving, purifying and drying to form fimbrin; dissolving together with collagen or gelatin in the same kind of solvent; high voltage electrostatic spinning to obtain 3D netted nanometer fiber non-woven felt of fimbrin base material, collagen, gelatin, etc. and final organic alcohol treatment. The cell culturing rack material has average fiber diameter smaller than 100 nm, average pore size of 1.0-5.0 microns and porosity of 70-90%. Test shows that the nanometer fiber material has no toxicity, no irritation, no sensibilization, excellent tissue compatibility and perforated pore structure, and may be used as cell culturing rack material for repairing human body tissue.

The present invention relates to the processes of preparing silkfibroin / polyethylene oxide blended materials, and the resulting materials thereof, which are suitable for biomedical applications such as wound healing. In particular, the electrospun silk fibroin / PEO mats with a silk:PEO blend ratio of 2:1 to 4:1, treated with controlled evaporation, constraint-drying techniques, and / or alcohol treatment, and / or PEO extraction, demonstrate suitable physical and biofunctional properties, such as fiber structure, topography, absorption, water vapor transmission rates, oxygen permeation, and biodegradability, relevant to biomaterial systems with utility for wound dressings.

A method is provided for substantially instantaneously wetting hydrophobic, porous polymeric membranes and for rendering hydrophobic membranes hydrophilic. The method involves treating the membrane with a non-alcoholic aqueous solution of a low molecular weight surfactant, and then drying the treated membrane. The low molecular weight surfactant exhibits high polymer affinity for the hydrophobic membrane substrate as well as high water solubility; a preferred surfactant is sodium dodecylbenzenesulfonate (SDBS). The method is particularly useful for treating hydrophobic membranes such as those made of polyolefins, fluorinated or chlorinated polymers, polysulfone, or polyethersulfone, preferably having a pore size of about 0.01 microns to about 1 micron. A wettable membrane is thus provided as the aqueous surfactant solution is absorbed into the hydrophobic membrane.

The invention relates to a method for removing and inhibiting fishy smell of aquatic animal enzymatic hydrolysate. Edible grade alcohol is added into the aquatic animal enzymatic hydrolysate to conduce heating reaction for removing the fishy smell, edible grade alcohol treatment liquid is obtained, then, green tea extracts, water-soluble ginger oil and citric acid are added into the edible grade alcohol treatment liquid, or marigold extracts, water-soluble ginger oil and citric acid are added into the edible grade alcohol treatment liquid, primary fishy smell eliminating treatment liquid is obtained after the reaction, then, green tea extracts and water-soluble ginger oil are added into the primary fishy smell eliminating treatment liquid, or the marigold extracts and the water-soluble ginger oil are added into the primary fishy smell eliminating treatment liquid, and the aquatic animal enzymatic hydrolysate after the fishy smell elimination is obtained after the reaction. The method has the advantages that the fishy smell eliminating effect is good, the nutrition and active ingredients in the aquatic animal enzymatic hydrolysate are not basically lost, in addition, the anti-bacterium fresh keeping effect is good, the operation is simple, the equipment requirement is low, the reaction condition is mild, the control is easy, and the method is suitable for the fields of sea functional food, nutrition products, medicine, cosmetics and the like.

The invention provides an extracting method of microbial oil and short chain alcohol fatty ester, comprising: water content regulation: wet cultures which contain microbial oil and the water content of which is 20-90 percent are obtained; microwave treatment: the microwave irradiation is carried out on the wet cultures so that the water content lowers to 5-40 percent; short chain alcohol treatment: the alcoholysis is carried out on the oil in part of microbial bodies under the function of alkaline catalyst, and the oil is extracted simultaneously; solvent recovery: the solid-liquid separation is carried out, and mixture of the microbial oil and the short chain alcohol fatty ester is obtained after the short chain alcohol is recovered through the evaporation. The method has high extracting efficiency and the microbial oil and the short chain alcohol fatty ester can be obtained simultaneously.

The invention provides a preparation method of a high-strength low-carbon environment-friendly rigid silicon dioxide aerogel insulating board, which comprises the following steps: (1) taking 68-120g of sodium water glass with the modulus of 3.1-3.6, adding 100-200g of pure water, and stirring uniformly to obtain diluted water glass; (2) weighing 25-100g of 40 wt% sulfuric acid, adding 10-40g of ethanol, and stirring uniformly to obtain a sulfuric acid solution; (3) mixing the diluted water glass and sulfuric acid solution, and regulating the pH value to 3.5-4.5 to obtain a sol; (4) pouring the sol into a reinforcing-fiber-filled mold, gelatinating the sol after 10-15 minutes, and standing for 2-10 hours while keeping at the temperature of 50-80 DEG C, thereby obtaining a gel board; (5) carrying out integrated aging and acid alcohol treatment on the alcoholic aquagel board; (6) putting the gel board subjected to acid alcohol treatment into 1000-250ml of trimethylethoxy silane, treating at room temperature, and completing hydrophobic modification and gel drainage in one step within 6-10 hours; and (7) after the drainage is finished, drying the wet gel board with hot nitrogen or carbon dioxide.

A method for preparing biological section of sticking type includes using epoxy as currier of section as one carrier enabling to carry more than hundred sections, dewaxing, staining, using absolute ethyl alcohol to carry out alcohol treatment with concentration gradient from 95% to 50% after wax is dissolved by Xylol, carrying out alcohol treatment with concentration gradient from 50% to absolute ethyl alcohol after section is strained, carrying out centralized transmittance by Xylol, packaging section by utilizing resin jetting film process, drying and cutting to obtain finished section.

The invention discloses a preparation method of a silk fibroin porous material, which comprises the following steps: drying a silk fibroin aqueous solution into a silk fibroin membrane, dissolving the silk fibroin membrane into water; repeating the drying-dissolving process to obtain a silk fibroin nano-wire aqueous solution; performing freeze drying of the silk fibroin nano-wire aqueous solution to obtain a silk fibroin material precursor; performing alcohol treatment or vacuum water treatment of the silk fibroin material precursor, drying to obtain the silk fibroin porous material. Compared with the prior art, the preparation method of the invention regulates the nano-structure by the self-assembly behavior of silk fibroin, that is, during multiple drying-dissolving processes, the internal nano-sphere structure of the silk fibroin aqueous solution is self-assembled into a nano-wire structure, which effectively inhibits the formation of a sheet structure; the prepared silk fibroin porous material has a nano-fiber structure, and a high porosity.

A method for cleaning a dielectric and metal structure within a microelectronic structure uses an oxygen containing plasma treatment, followed by an alcohol treatment, in turn followed by an aqueous organic acid treatment. Another method for cleaning a dielectric and metal structure within a microelectronic structure uses an aqueous surfactant treatment followed by an alcohol treatment and finally followed by an aqueous organic acid treatment. The former method may be used to clean a plasma etch residue from a dual damascene aperture. The second method may be used to clean a chemical mechanical polish planarizing residue from a dual damascene structure. The two methods may be used sequentially, absent any intervening or subsequent sputtering method, to provide a dual damascene structure within a microelectronic structure.

The invention discloses silk fibroins with different degradation rates and a use thereof. Natural silk fibers are treated by lithium bromide, calcium chloride or acid-base to form silk fibroins degraded at a slow rate, a middle rate and a fast rate, and the silk fibroins are freeze-dried and molded and then is subjected to alcohol treatment so that silk fibroin supports with different degradation rates are obtained. Under different hydrolysis conditions, a silk fibroin degradation rate can be effectively controlled, and in nerve injury restoration, a nerve revegetation rate is equal to a support material degradation rate. A research proves that the silk fibroin supports with fast and medium degradation rates can promote nerve damage restoration and has good curative effects on peripheral nerve damage treatment.

The invention discloses a method for preparing epsilon-caprolactam through cyclohexanone-oxime gas-phase Beckmann rearrangement. In nitrogen atmosphere, a low-carbon alcoholic solution of cyclohexanone-oxime is in contact with a catalyst bed for rearrangement reaction, and a product is recovered. The method is characterized in that the method is carried out in a fixed-bed reactor; when the conversion rate of the cyclohexanone-oxime is lower than 99.0 percent and / or when the BET specific surface area of a catalyst decreases to be or lower than 70 percent of that of a fresh catalyst, the low-carbon alcoholic solution of the cyclohexanone-oxime is changed into a low-carbon alcohol treatment catalyst bed; the low-carbon alcohol treatment catalyst bed is swept at a temperature between 350 and 550 DEG C by use of oxygen-containing atmosphere; and after surface coking matter of catalysts is burnt out and the BET specific surface area of the catalysts is basically recovered, the low-carbon alcohol treatment catalyst bed is in contact with a buffer solution of nitrogen-containing compound at a temperature between 50 and 120 DEG C, and is washed and dried, and then reaction continues to be performed.

The invention discloses waste alcohol coal-slurry as well as the preparation method and the application thereof. The components and parts by weight are as follows: 100-180 potions of coal powder, 4-99 portions of waste alcohol, 0-90 portions of water and 0.1-3 portions of dispersing agent. The preparation method of the waste alcohol coal-slurry has simple operation because the waste alcohol is directly used without the pretreatment of concentration or solid-liquid separation, and the like, shortens the waste alcohol treatment period, lowers the energy consumption, and does not basically produce the secondary pollution. The waste alcohol can replace partial coal and water, thereby saving resources. The waste alcohol coal-slurry is placed for a long time without producing hard settlement, has good fluidity so as to be suitable for being transported by a pump, and can be used as fuel for combustion or as gasified raw material for preparing gas.

The invention provides a mussel polysaccharide. The polysaccharide content of the mussel polysaccharide is detected by an ultraviolet spectrophotometer and the polysaccharide is 55 to 90 percent based on glucose (C6H12O6). Monosaccharide components in the mussel polysaccharide, which are detected by high performance liquid chromatography, comprise the glucose, glucuronic acid, glucosamine and the like. The mussel polysaccharide is prepared by alcohol treatment, water extraction, pressure filtration, drying and other processes. Clinical verification results show that the mussel polysaccharide has definite curative effect of treating patients with syndrome of endogenous heat due to yin deficiency and the syndrome of endogenous heat due to yin deficiency accompanied with cancer pain, is convenient to take and safe, does not have any toxic or side effect and can be used for preparing medicaments for nourishing yin, clearing heat, diminishing swelling and relieving pain and the cancer pain.

The invention relates to a device for recovering disulfide from condensate oil desulfurized alcohol and alkali liquor, which comprises a desulfurized alcohol treatment unit, wherein a bottom end outlet of the desulfurized alcohol treatment unit is communicated with a bottom end inlet of an alkali liquor oxidization and sulfur dioxide separation unit; a bottom end outlet of the alkali liquor oxidization and sulfur dioxide separation unit is communicated with a side end inlet of an alkali liquor gas stripping column; a top end outlet of the alkali liquor oxidization and sulfur dioxide separation unit and a top end outlet of the alkali liquor gas stripping column are respectively communicated with a side end inlet of an exhaust water washing dealkalization tank; a side end outlet of the alkali liquor oxidization and sulfur dioxide separation unit and a bottom end outlet of the exhaust water washing dealkalization tank are respectively communicated with a top end inlet of a disulfide tank; a bottom end outlet of the alkali liquor gas stripping column is communicated with a side end inlet of the desulfurized alcohol treatment unit; and the device also comprises one or a plurality of filters connected in the components above, a plurality of circulating pumps, a plurality of pipelines, a plurality of liquid inlets and a plurality of liquid outlets.

The present invention provides a method of substantially instantaneously wetting a hydrophobic porous polymer membrane and rendering the hydrophobic membrane hydrophilic. The method involves treating the membrane with a non-alcoholic aqueous solution of a low molecular weight surfactant, followed by drying the treated membrane. The low molecular weight surfactant has high molecular affinity to the hydrophobic membrane matrix and high water solubility; the preferred surfactant is sodium dodecylbenzenesulfonate (SDBS). The method is particularly suitable for the treatment of hydrophobic membranes such as those made of polyolefins, fluorinated or chlorinated polymers, polysulfone or polyethersulfone, preferably having a pore size of 0.01 micron to 1 micron. The present invention provides a wettable membrane because the aqueous surfactant solution is absorbed into the hydrophobic membrane.

The present invention relates to the processes of preparing silkfibroin / polyethylene oxide blended materials, and the resulting materials thereof, which are suitable for biomedical applications such as wound healing. In particular, the electrospun silk fibroin / PEO mats with a silk:PEO blend ratio of 2:1 to 4:1, treated with controlled evaporation, constraint-drying techniques, and / or alcohol treatment, and / or PEO extraction, demonstrate suitable physical and biofunctional properties, such as fiber structure, topography, absorption, water vapor transmission rates, oxygen permeation, and biodegradability, relevant to biomaterial systems with utility for wound dressings.

The invention provides a method for regulating and controlling photoelectric haze and an ultraviolet filtration effect of a modified nano-cellulose film. The modified nano-cellulose film is obtained by modifying TEMPO-oxidized cellulose nanofiber through a Zn(II) terpyridine complex; the modified nano-cellulose film is put into an alcohol solvent, soaked and then taken out, excessive solvents areremoved, and thus the modified nano-cellulose film subjected to solvent treatment is obtained; and then the modified nano-cellulose film subjected to solvent treatment is put into water, soaked for 10-150 min and then taken out, excessive water is removed, and thus the modified nano-cellulose film subjected to water treatment is obtained. According to the method, the haze value of the modified nano-cellulose film can be regulated and controlled through alcohol treatment, specifically, after alcohol treatment, the haze is increased, ultraviolet filtration performance is improved, the modified nano-cellulose film can be subjected to haze adjusting according to the different needs, and the using range of the modified nano-cellulose film is expanded.

The invention provides a method for manufacturing probiotic living bacterium kvass by an alcohol treatment-composite inhibitor method and belongs to the technical field of kvass manufacture methods. The method comprises the steps of plant extraction, kvass bread production, dehydrated bread extraction, medium supply two-segment type microbial fermentation, centrifugal bacterium collection, alcohol treatment and washing, preparation, filling and the like. According to the method, a two-segment fermenting process of sequentially high and low fermenting temperatures is adopted in the aspect of microbial fermentation; microbial cells can be dormant by means of centrifugal bacterium collection and alcohol treatment and is additionally mixed with a compound plant extract to guarantee the product flavor and bio-stability, so that the aromatic ingredient and flavoring substances in kvass can be furthest maintained, the kvass product produced by the method can especially achieve a preservation period of 20 months without adding preservative. The kvass product manufactured by the method has rich bread smell, tastes smooth and refresh, has a long preservation period, and is an upgrading product of kvass.

The invention provides polypropylene-polyester-carbon nano tube ternary composite alloy and a preparation method thereof. The preparation method includes the steps that 1, an alkene monomer, dual-functional carbon nano tubes and a promoter are used for undergoing an in-situ coordination polymerization reaction, so that an intermediate product is obtained, wherein the alkene monomer comprises a propylene monomer and a mixture of the propylene monomer and ethylene and / or 1-butene; 2, the intermediate product is subjected to acid alcohol treatment, hydroxyl used for modifying a polyolefin catalyst is made to be recovered, in the existence of the catalyst system, the recovered hydroxyl is used as an initiator to trigger ring-opening polymerization of cyclic ester monomers, and accordingly the polypropylene-polyester-carbon nano tube ternary composite alloy is obtained. According to the polypropylene-polyester-carbon nano tube ternary composite alloy and the preparation method thereof, polypropylene and polycaprolactone are used for establishing strong interfacial force through the chemical attachment of the carbon nano tubes, and the polycaprolactone and the carbon nano tubes are stably dispersed in a polypropylene matrix at the same time. The polypropylene has a good mechanical property and a good thermal property, has good wettability, printing and dyeing properties and the compatibility with other polar polymers, and further has certain biocompatibility, and thus the polypropylene is turned into a high performance resin material with excellent overall performance.

Methods of preparing calcification-resistant bioprosthetic tissue include providing fresh biological tissue, cross-linking the tissue, treating the cross-linked tissue with an alcohol for a time sufficient to allow the alcohol to be diffused into the tissue, and treating the alcohol-treated fixed tissue with a polyol for a time sufficient to allow fluid in the tissue to be replaced by the polyol. The methods may include sterilizing the cross-linked tissue in a solution including propylene oxide or peracetic acid either before or after the alcohol treatment step; or drying the alcohol / polyol-treated, cross-linked tissue, sterilizing the dried tissue by exposure to ethylene oxide or peracetic acid, and storing the sterilized tissue in a dry, ambient environment. The treated tissue may be a tissue component for a bioprosthetic valve, a valve assembly for a bioprosthetic valve or a fully assembled bioprosthetic valve incorporating the tissue.

The metallization method of thermosetting resin bimaleimide resin-glass fibre composite material includes pretreatment of material surface and process of plating copper or plating nickel or plating gold or plating tin-lead alloy, and the described pretreatment includes the procedures of degreasing, cleaning, pre-etching, alcohol treatment, etching, activating, reducting and chemically-plating. The etching treatment is implemented by means of acid treatment, water washing, more acid treatment, water washing, nitrating, mixed alcali treatment and acid washing. Between metal cladding layer and base body they have good combined state.

The invention discloses a making method of shortcakes containing ginkgo whole powder. The making method comprises the steps of preparing materials, mixing the prepared materials, performing shaping, performing decoration, performing baking, performing cooling, performing transportation, performing sorting and performing packaging. According to the making method disclosed by the invention, ginkgo powder is added to foods, so that the practicability of ginkgoes is improved; the ginkgo whole powder is used as a functional medium raw material to be applied to foods, so that people can convenientlyand better absorb the efficacy of the ginkgoes and the nutrients of other components when eating the shortcakes, and the medical-care and medical value and the dietetic therapy and dietary value of the ginkgoes are effectively utilized; the ginkgo powder is subjected to high-temperature pre-gelatinizing treatment, so that poisonous substances are passivated, and the detoxification effect is achieved; besides, through combination of alcohol treatment and a diluted alkali neutralization technique, the detoxification effect is further improved, the eating safety of the ginkgo powder is guaranteed, and the ginkgo powder is convenient to popularize; and the ginkgo powder is dehydrated and dried through a drum, so that functional components of ginkgo products can be reserved to the maximum extent. The shortcakes have good competitiveness at home and abroad and have favorable industrialization prospects.

The invention discloses a method for preparing water-soluble titanium dioxide. The method comprises the following steps: 1) preparing a titanium compound solution with the concentration of 5-50 wt%; 2) uniformly mixing the titanium compound solution, alcohol, inorganic acid and organic acid to obtain a mixed solution; 3) stirring the mixed solution at 90-130 DEG C to obtain a heat treatment solution; 4) adding deionized water and alcohol into the heat treatment solution, and uniformly mixing all the components to obtain an alcohol treatment solution; 5) adding a modifier into the alcohol treatment liquid to obtain a dispersion liquid; 6) stirring the dispersion liquid at 120-150 DEG C, and then adding alcohol to obtain sol; 7) drying and grinding the sol to obtain the water-soluble titanium dioxide. According to the method, subsequent calcination and addition of a dispersing agent are not needed, the prepared water-soluble titanium dioxide is directly dispersed in water to form a transparent sol, the dispersity is good, and a film formed by the prepared water-soluble titanium dioxide and a water-based coating prepared from the water-soluble titanium dioxide have photocatalytic properties.

The present invention discloses a papaya long time fresh-keeping method characterized by comprising the following steps: (1) preservative preparation; (2) papaya pretreatment; (3) radiation treatment; and (4) fumigation packaging. The preservative contains preservative powder and vinclozolin, and has good preservation effect, sodium benzoate and citric acid have anti-brown effect, sodium chloride extends the shelf life, meanwhile supplements the proper amount of vitamin; before harvest, melon body inside infection of pathogenic bacteria can be prevented to a large extent by alcohol treatment to melon vines, the disease severity of postharvest papaya can be reduced, the waste rate of post-harvest transport, storage and other aspects can be reduced, and the method combining the preservative and ionizing radiation can make papaya bright in fruit color and good in taste, and has a good astringency removal effect.

The invention provides a preparation method of a decellularized biological amnion. The preparation method comprises cleaning, chemical treatment and freeze drying of the amnion. According to the method, a fresh amnion is washed clean with normal saline, residual blood on the amnion is washed away, the obtained amnion is treated with hydrogen peroxide, acetone, sodium dodecyl sulfate and alcohol again, after alcohol treatment, the obtained amnion is subjected to profound hypothermia freezing in an environment at subzero 70 DEG C for 16-25 h, and the obtained amnion is placed in a freezer dryer for freeze drying according to a set freeze drying curve. A finished decellularized biological amnion is obtained after irradiation sterilization. The amnion is jointly treated with hydrogen peroxide, acetone, sodium dodecyl sulfate and alcohol, immunogenic ingredients of an epithelial cell layer, a fibrous membrane cell layer and a sponge layer in the amnion can be effectively removed, meanwhile, a basilar membrane and a compact layer of the amnion are reserved, and the biological activity and biomechanical properties of the amnion are better reserved; on the other hand, the immunogenicity of the decellularized biological amnion can be further reduced through freeze drying treatment, and the immunological rejection of host cells is reduced. In addition, the preparation method of the decellularized biological amnion is simple and easy to implement, and large-scale production operation is facilitated.

Methods of preparing calcification-resistant bioprosthetic tissue include providing fresh biological tissue, cross-linking the tissue, treating the cross-linked tissue with an alcohol for a time sufficient to allow the alcohol to be diffused into the tissue, and treating the alcohol-treated fixed tissue with a polyol for a time sufficient to allow fluid in the tissue to be replaced by the polyol. The methods may include sterilizing the cross-linked tissue in a solution including propylene oxide or peracetic acid either before or after the alcohol treatment step; or drying the alcohol / polyol-treated, cross-linked tissue, sterilizing the dried tissue by exposure to ethylene oxide or peracetic acid, and storing the sterilized tissue in a dry, ambient environment. The treated tissue may be a tissue component for a bioprosthetic valve, a valve assembly for a bioprosthetic valve or a fully assembled bioprosthetic valve incorporating the tissue.

The invention discloses a paraffin section method for pomegranate seed particles. The paraffin section method comprises the following steps: (1) material selection and fixing; (2) dehydration and transparency: carrying out gradient dehydration, carrying out treatment for 2 h in each gradient, and then converting into absolute ethyl alcohol treatment for 1.5 h twice; adding a mixed solution of absolute ethyl alcohol and xylene in a volume ratio of 2: 1 and a mixed solution of absolute ethyl alcohol and xylene in a volume ratio of 1: 2 for 2 h, and then converting into pure xylene treatment for 1.5 h twice; (3) waxing and embedding: putting the transparent materials into a mixed solution of xylene and paraffin in a volume ratio of 1: 1 for waxing for 4 to 5 h, then putting in a mixed solution of xylene and paraffin in a volume ratio of 1: 2 for waxing for 4 to 5 h, then waxing for 4 to 5 h with pure paraffin, and then continuously waxing for 3 to 4 d by changing the pure paraffin; (4) section and spreading; and (5) dyeing to be transparent, and mounting. A section tissue manufactured by the method is complete in structure and clear in cell boundary, and the sections are clean and easy to observe.