Paraffin section method for pomegranate seed particles

A technology of paraffin sectioning and pomegranate, which is applied in the field of paraffin sectioning of pomegranate seeds, which can solve the problems of difficult to obtain intact and clear microsections, and achieve the effects of clear cell boundaries, clean sections, and easy observation

Active Publication Date: 2017-09-26
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large structure difference between the inner and outer testa of pomegranate, the outer testa is thick and juicy, and the inner testa is rich in lignin, cellulose, hemicellulose, etc., an

Method used

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  • Paraffin section method for pomegranate seed particles

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Embodiment 1

[0023] A kind of paraffin section method of pomegranate grain, comprises the steps:

[0024] Step 1. Material collection and fixation: Select 3 to 4 fresh, undamaged pomegranate fruits, put them in an ice box, and transport them back to the laboratory. Take out 100 whole and undamaged pomegranate seeds, put them into FAA fixative solution immediately and fix them for more than 24 hours. Wherein, the FAA fixative is configured by 90 parts by volume of 70% ethanol, 5 parts by volume of glacial acetic acid, and 5 parts by volume of 38% formalin.

[0025] Step 2. Dehydration and transparency: Put the fixed pomegranate seeds into 70% ethanol, 80% ethanol, 90% ethanol, and 95% ethanol in sequence for gradient dehydration. Each gradient treatment is 2 hours each, and then transferred to absolute ethanol for 1.5 hours , and then replace the absolute ethanol treatment for 1.5h, and the dehydration is completed; the dehydrated pomegranate seeds are put into the mixed solution of absolu...

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Abstract

The invention discloses a paraffin section method for pomegranate seed particles. The paraffin section method comprises the following steps: (1) material selection and fixing; (2) dehydration and transparency: carrying out gradient dehydration, carrying out treatment for 2 h in each gradient, and then converting into absolute ethyl alcohol treatment for 1.5 h twice; adding a mixed solution of absolute ethyl alcohol and xylene in a volume ratio of 2: 1 and a mixed solution of absolute ethyl alcohol and xylene in a volume ratio of 1: 2 for 2 h, and then converting into pure xylene treatment for 1.5 h twice; (3) waxing and embedding: putting the transparent materials into a mixed solution of xylene and paraffin in a volume ratio of 1: 1 for waxing for 4 to 5 h, then putting in a mixed solution of xylene and paraffin in a volume ratio of 1: 2 for waxing for 4 to 5 h, then waxing for 4 to 5 h with pure paraffin, and then continuously waxing for 3 to 4 d by changing the pure paraffin; (4) section and spreading; and (5) dyeing to be transparent, and mounting. A section tissue manufactured by the method is complete in structure and clear in cell boundary, and the sections are clean and easy to observe.

Description

technical field [0001] The invention relates to the technical field of paraffin sectioning of plants, in particular to a method for paraffin sectioning pomegranate grains. Background technique [0002] Pomegranate (Punica granatum) is a deciduous shrub or small tree of the genus Punica in the Lythraceae family. It is native to Iran and Afghanistan. . Pomegranate fruit is a round or oval fruit wrapped in a leathery peel. There are 5-7 ventricles inside the peel, and each ventricle is composed of dozens to hundreds of seeds. The seeds are the edible parts of the fruit, which is different from apples, Pears, strawberries, peaches, plums, apricots, and longan are all different. The pomegranate seed coat is composed of two layers of inner and outer seed coats. The outer seed coat is thick and juicy, rich in sugar, organic acids, polyphenols, etc., and is the main edible part; the inner seed coat is rich in lignin, cellulose, hemicellulose, etc. It is the main factor of grain h...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/06
Inventor 秦改花徐义流刘春燕张金云高正辉齐永杰潘海发伊兴凯张晓玲
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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