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Technique for fabricating label type biologic slice

A production process and slicing technology, which is applied in the field of label-type biological slice production process, can solve the problems of low production efficiency, poisoning, and affecting the consistency of staining and sealing of sliced ​​specimens, so as to improve production efficiency and reduce environmental and human pollution and harm, product novelty effect

Inactive Publication Date: 2007-02-21
段增强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological slice production process is limited by the carrier, and the sliced ​​specimens cannot be processed centrally. Each slide can only hold one to several sliced ​​specimens. Due to the limitation of the slides, this affects the sliced ​​specimens to a certain extent. Consistency of staining and sealing, and more importantly, only one specimen can be produced at a time, resulting in low production efficiency
Thirdly, the producers have been poisoned by xylene and other harmful drugs for a long time, and it has not been solved so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] The Existing Biological Slicing Technology and Its Deficiencies (Taking Paraffin Section as an Example)

[0011] (1) The main steps of the existing bioslicing technology

[0012] Material collection: It depends on the purpose of production, but whether it is animal or plant organ materials, the principle of "small but precise" should be followed when collecting materials to facilitate fast fixation.

[0013] Fixation: Quickly kill and fix the collected materials with formaldehyde, glacial acetic acid, and ethanol liquid to keep them in their original living state.

[0014] Washing with water: After the material is killed and fixed, wash away the fixative in the material with water or 50% ethanol washing solution.

[0015] Dehydration: Use one of ethanol, n-butanol, tert-butanol, and acetone to replace all the moisture in the material.

[0016] Transparent: After the material is dehydrated, xylene is often used as a transparent agent, because it can be mixed with both ...

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Abstract

A method for preparing biological section of sticking type includes using epoxy as currier of section as one carrier enabling to carry more than hundred sections, dewaxing, staining, using absolute ethyl alcohol to carry out alcohol treatment with concentration gradient from 95% to 50% after wax is dissolved by Xylol, carrying out alcohol treatment with concentration gradient from 50% to absolute ethyl alcohol after section is strained, carrying out centralized transmittance by Xylol, packaging section by utilizing resin jetting film process, drying and cutting to obtain finished section.

Description

technical field [0001] The invention relates to a biological slicing technology, in particular to a label-type biological slicing manufacturing process. Background technique [0002] The traditional bioslicing technology was introduced into my country in the early 20th century, and has played an important role in the fields of biological research and teaching for a long time. my country has successively established many biological slice specimen factories, providing a large number of biological, medical and other sliced ​​specimens for agricultural, medical, and biological colleges and universities and primary and secondary schools across the country. However, the existing biological slice production process is limited by the carrier, and the sliced ​​specimens cannot be processed centrally. Each slide can only hold one to several sliced ​​specimens. Due to the limitation of the slides, this affects the sliced ​​specimens to a certain extent. Consistency of staining and sea...

Claims

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Application Information

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IPC IPC(8): G01N1/28G09B23/00
Inventor 段增强高致明贺德先田朝阳
Owner 段增强
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