Silk fibroin porous material and preparation method thereof and tissue engineering stent

Abstract

The invention discloses a preparation method of a silk fibroin porous material, which comprises the following steps: drying a silk fibroin aqueous solution into a silk fibroin membrane, dissolving the silk fibroin membrane into water; repeating the drying-dissolving process to obtain a silk fibroin nano-wire aqueous solution; performing freeze drying of the silk fibroin nano-wire aqueous solution to obtain a silk fibroin material precursor; performing alcohol treatment or vacuum water treatment of the silk fibroin material precursor, drying to obtain the silk fibroin porous material. Compared with the prior art, the preparation method of the invention regulates the nano-structure by the self-assembly behavior of silk fibroin, that is, during multiple drying-dissolving processes, the internal nano-sphere structure of the silk fibroin aqueous solution is self-assembled into a nano-wire structure, which effectively inhibits the formation of a sheet structure; the prepared silk fibroin porous material has a nano-fiber structure, and a high porosity.

Description

technical field [0001] The invention relates to the technical field of materials, and more specifically relates to a silk fibroin porous material, a preparation method thereof and a tissue engineering scaffold. Background technique [0002] The defect or failure of cells and tissues caused by various diseases has become a major problem affecting human health. Promoting or guiding cell and tissue regeneration through foreign media has gradually become an effective method to solve the above problems. Among them, selecting a suitable tissue engineering scaffold material as a carrier for cell and tissue regeneration is a core issue in the field of regenerative medicine. Therefore, the performance of the scaffold material is of great significance for the treatment of tissue defects. [0003] Scaffold materials used in tissue repair are required to have excellent cell and tissue compatibility, be able to induce cell differentiation and promote normal tissue regeneration. At prese...

AI Technical Summary

Problems solved by technology

For example, "Three-dimensional aqueous-derived biomaterial scaffolds" published in "biomaterial" by Ung-Jin Kim et al. and "The synergistic effects of 3-D porous silkfibroin matrix scaffold properties and hydrodynamic environment in Cartilaget issue regeneration” involves the preparation and application of porous silk fibroin scaffold materials, but neither the crystal structure nor the nanostructure of silk fibroin is regulated, which limits its application in various fields

The Chinese patent document with the publication number CN1844509A reports a method for preparing a silk fibroin porous structure material, which uses electrospinning to prepare a porous scaffold, but its pore size is small, and it is also difficult to obtain a porous scaffold with a complex structure

The Chinese patent literature with the publication number CN101703795A reports a method for preparing a nano-calcium tricalcium phosphate silk protein composite three-dimensional porous material, and introduces a method for preparing a porous silk fibroin scaffold by the salting-out method. Because this technical solution requires the use of concentrated salt solution , so the obtained silk fibroin porous scaffold is not conducive to the loading of drugs; at the same time, because the secondary structure of the obtained scaffold is a high content silk fibroin II structure, the degradation performance cannot be controlled

In addition, in the prior art, the Chinese patent literature with the publication number CN101502669A reports the silk fibroin porous three-dimensional scaffold and its preparation method. The three-dimensional scaffold is prepared by the freeze-drying method, which avoids the use of organic solvents, but the porosity of the scaffold is not high. presence of separate lamellar structures

Images