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Preparation method of controlled-release oriented nanofiber nerve conduit

A technology of nerve conduits and nanofibers, applied in the direction of catheters, etc., can solve the problem of limited repair effect of a single nerve conduit, achieve good biocompatibility and biodegradability, simple and easy preparation method, and broad application prospects

Inactive Publication Date: 2011-06-15
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, nerve regeneration is the result of the interaction of cells, extracellular matrix and neurotrophic factors, and the repair effect of a single nerve conduit is relatively limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] First add 100 grams of de-chrysalis cocoons to 1 liter of 0.5w / v% Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, repeating the treatment three times, fully washing with distilled water, and drying in a drying oven at 45° C. to obtain degummed silk fibers. with CaCl 2 : C 2 h 5 OH:H 2 A ternary solvent was prepared at a molar ratio of O=1:2:8, and the silk fiber was hydrolyzed at a constant temperature in a water bath at 70° C. for 1 hour at a bath ratio of 1:10 to obtain a completely dissolved brown-yellow silk fiber hydrolysis solution. Put the hydrolyzed solution into a dialysis bag, dialyze with distilled water for 72 hours, put the dialyzed silk fiber hydrolyzed solution in -80°C for 12 hours, and then freeze-dry it at -58°C until dry to obtain a white, odorless, loose porous solid silk fibroin.

[0016] Weigh 0.5g silk fibroin, 0.01mg nerve growth factor (NGF) and 0.01mg glial cell-derived neurotrophic factor (GDNF) respectively with an electronic b...

Embodiment 2

[0019] Weigh 1.0 g of silk fibroin, 0.01 mg NGF and 0.01 mg FGF prepared according to Example 1 with an electronic balance, and dissolve them in 10 mL of sterile deionized water, stir until completely dissolved to obtain 10 w / v% silk fibroin Core layer solution: Weigh 0.2g silk fibroin and 0.6gP(LLA-CL) (50:50) respectively, dissolve in 10mL of hexafluoroisopropanol, stir until completely dissolved, and obtain a mass ratio of solute of 25:75 , a mixed solution with a total concentration of 8w / v% was used as the cortex solution. Coaxial electrospinning conditions: set the feeding rate of the core layer solution to 0.2mL / h, the diameter of the spinneret to 0.9mm, the feeding rate of the cortex solution to 1.0-1.2mL / h, and the diameter of the spinneret to 1.2 mm; the voltage is 10-15kv, the receiving distance is 12cm; the receiving rate of the high-speed rotating drum is 4000rpm, and the oriented nanofiber film is obtained.

[0020] The prepared oriented nanofiber membrane was c...

Embodiment 3

[0022] Take by weighing 1.5g silk fibroin, 0.01mg nerve growth factor (NGF) and 0.01mg fibroblast growth factor (FGF) prepared according to Example 1 respectively with electronic balance, and dissolve it in 10mL sterile deionized water , stirred until completely dissolved to obtain a 15w / v% silk fibroin core layer solution; weigh 0.15g silk fibroin and 0.45g P(LLA-CL) (50:50) respectively, and dissolve them in 10mL of hexafluoroisopropanol , stirred until completely dissolved to obtain a mixed solution with a mass ratio of solutes of 25:75 and a total concentration of 6w / v% as a skin layer solution. Coaxial electrospinning conditions: set the feeding rate of the core layer solution to 0.2mL / h, the diameter of the spinneret to 0.9mm, the feeding rate of the cortex solution to 1.0-1.2mL / h, and the diameter of the spinneret to 1.2 mm; the voltage is 10kv, the receiving distance is 12cm; the receiving rate of the high-speed rotating drum is 4000rpm, and the oriented nanofiber film...

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Abstract

The invention relates to a preparation method of a controlled-release oriented nanofiber nerve conduit. The method comprises the following steps: preparing a core layer solution, preparing a skin layer solution, performing coaxial electrospinning, preparing a controlled-release oriented nanofiber membrane with a high-speed rotary drum receiving device and preparing the nanofiber nerve conduit. The preparation method in the invention has advantages of simple and practical, rich raw material sources, good biocompatibility and biodegradability, and is easy to realize industrial production. The prepared nerve conduit has wide application prospects in the clinical treatment of peripheral nerve damage.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a preparation method of a slow-release oriented nanofiber nerve guide. Background technique [0002] Peripheral nerve injury is a common clinical disease with poor prognosis and high disability rate, which brings huge economic loss and mental burden to patients, families and society. For peripheral nerve defects, autologous nerve transplantation is still the "gold standard" for repair. However, there are disadvantages such as slow regeneration rate, limited sources of autologous nerve materials, and mismatch of donor and recipient nerve diameters. Therefore, it is the key to nerve repair to find substitutes for autogenous nerves and prepare ideal nerve guides to promote nerve regeneration. An ideal nerve conduit scaffold should meet: ① the structure and biological function of bionic natural extracellular matrix; ② good biocompatibility so that cells can adhere and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L29/12A61L29/16
Inventor 张葵花莫秀梅汪春阳
Owner JIAXING UNIV
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