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Directional fibrin and polypeptide interpenetrating network composite hydrogel for nerve regeneration and preparation method thereof

A composite hydrogel and fibrin technology, applied in the field of oriented fibrin and polypeptide interpenetrating network composite hydrogel and its preparation, can solve the problems of short half-life, fast diffusion, etc., to promote angiogenesis and nerve cell adhesion , to avoid unpredictable and unnecessary physiological or pathological effects

Pending Publication Date: 2021-03-19
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many problems in the clinical application of exogenous growth factors
On the one hand, most growth factors are rapidly degraded in the body due to their short half-lives and rapid diffusion, while on the other hand, there may be unknown safety issues when using high-dose growth factors

Method used

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  • Directional fibrin and polypeptide interpenetrating network composite hydrogel for nerve regeneration and preparation method thereof
  • Directional fibrin and polypeptide interpenetrating network composite hydrogel for nerve regeneration and preparation method thereof
  • Directional fibrin and polypeptide interpenetrating network composite hydrogel for nerve regeneration and preparation method thereof

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preparation example Construction

[0048] The preparation method of the oriented fibrin and functional polypeptide interpenetrating network composite hydrogel for nerve regeneration of the present invention comprises:

[0049] 1) Dissolving fibrinogen in pure water and mixing it evenly with polyethylene oxide aqueous solution to obtain an electrospinning stock solution, the mass fraction of fibrinogen in the electrospinning stock solution is 2%-8%, and the mass fraction of polyethylene oxide is 0.1%-0.8%, the electrospinning stock solution is injected into the syringe for use;

[0050] 2) Add CaCl 2 Mix the aqueous solution with the thrombin solution to obtain a cross-linking solution and keep it warm at 37°C, and the CaCl in the cross-linking solution 2 The mass fraction of thrombin is 0.5%-3%, the content of thrombin is 4-50Units / mL, and the cross-linking solution is placed in a metal rotating receiving tray for use;

[0051] 3) The electrospinning stock solution injected into the syringe is electrospun und...

Embodiment 1

[0062] 1) Dissolving fibrinogen in pure water and mixing it evenly with polyethylene oxide aqueous solution to obtain electrospinning stock solution. The mass fraction of fibrin in the electrospinning stock solution is 2%, and the mass fraction of polyethylene oxide is 0.5%. The electrospinning stock solution was then injected into a syringe for use.

[0063] 2) Add CaCl 2 The aqueous solution was mixed with the thrombin solution to obtain a cross-linking solution, which was then kept at 37°C for use. CaCl in the crosslinking solution 2The mass fraction of the thrombin is 0.5%, and the thrombin content is 4Units / mL. The cross-linking solution was then placed in a metal rotating receiving pan for use.

[0064] 3) The electrospinning stock solution injected into the syringe is electrospun under the action of the propulsion pump, the injection speed of the fibrinogen solution is adjusted to 2mL / h, and the loading voltage is 4kV to obtain continuous and stable fibrinogen spinn...

Embodiment 2

[0069] 1) Dissolving fibrinogen in pure water and mixing it evenly with polyethylene oxide aqueous solution to obtain electrospinning stock solution. The mass fraction of fibrin in the electrospinning stock solution is 2%, and the mass fraction of polyethylene oxide is 0.5%. The electrospinning stock solution was then injected into a syringe for use.

[0070] 2) Add CaCl 2 The aqueous solution was mixed with the thrombin solution to obtain a cross-linking solution, which was then kept at 37°C for use. CaCl in the crosslinking solution 2 The mass fraction of the thrombin is 0.5%, and the thrombin content is 4Units / mL. The cross-linking solution was then placed in a metal rotating receiving pan for use.

[0071] 3) The electrospinning stock solution injected into the syringe is electrospun under the action of the propulsion pump, the injection speed of the fibrinogen solution is adjusted to 2mL / h, and the loading voltage is 4kV to obtain continuous and stable fibrinogen spin...

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Abstract

The invention discloses a directional fibrin and polypeptide interpenetrating network composite hydrogel for nerve regeneration and a preparation method thereof, and belongs to the technical field ofbiomedical materials. The composite hydrogel is composed of a fibrin and polypeptide interpenetrating composite nanofiber structure. The directional fibrin hydrogel is prepared by electrostatic spinning, cross-linked polypeptide is interpenetrated with fibrin through swelling and physical adsorption, and then is stably combined with fibrin through a variety of cross-linking methods to form the directional interpenetrating network composite hydrogel. The composite hydrogel is a composite hydrogel with high water content, has the composition, structure and mechanical properties highly simulatingthe extracellular matrix of natural nerve tissues, contains polypeptide fragments simulating the function of growth factors, has excellent effects of promoting nerve cell adhesion and nerve fiber directional growth and inducing nerve and blood vessel regeneration, and can be applied to nerve regeneration repair of brain, spinal cord and peripheral nerve injury.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a composite hydrogel of directional fibrin and polypeptide interpenetrating network for nerve regeneration and a preparation method thereof. Background technique [0002] Nerve injury is a common clinical problem in the world, including central nerve injury and peripheral nerve injury. It is characterized by high incidence, high disability rate and younger age. It is a major problem that needs to be solved urgently in the medical field. Nerve injury seriously affects the normal life and family happiness of patients, and also brings a huge economic burden to the society. At present, there is no effective and convenient treatment method and method for nerve injury repair. The repair after nerve injury is a series of complex pathological processes. The slow nerve regeneration process, the formation of scar tissue, the generation of inhibited microenvironment...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/52A61L27/54A61L27/58
CPCA61L27/225A61L27/18A61L27/227A61L27/52A61L27/54A61L27/58A61L2430/32A61L2300/252A61L2300/412A61L2300/414A61L2400/12C08L71/02C08L89/00
Inventor 王秀梅曹峥杨淑慧杨嘉
Owner TSINGHUA UNIV
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