Kit for predicting colorectal cancer liver metastases and use method

A colorectal cancer and kit technology, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as delay in treatment time, expensive detection, and large human factors in identification, so as to prolong survival time and huge Clinical value, effect of improving quality of life

Inactive Publication Date: 2017-05-24
苏州首度基因科技有限责任公司 +1
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Imaging indicators are not objective enough, and the identification of some small tumor lesions is human-intensive, requiring doctors with rich clinical experience to identify them; liver metastases of tumors identified through imaging need to grow to a certain extent, and most of them are already in the late stage of liver metastases when th

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for predicting colorectal cancer liver metastases and use method
  • Kit for predicting colorectal cancer liver metastases and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0026] The method for predicting liver metastasis of colorectal cancer using the kit of the present invention comprises the following steps:

[0027] (1) Sample collection, collect 5ml of blood from the patient, store it in a vacuum blood collection tube, and extract cfDNA within 72 hours;

[0028] (2) DNA extraction, using blood DNA extraction kit to extract cfDNA in blood (which contains a small amount of DNA released by colorectal cancer tumor cells, carrying tumor cell-specific gene mutations);

[0029](3) Building a library, using a DNA library building kit to build a library for blood free DNA;

[0030] (4) On-machine sequencing, the Illumina next-generation sequencing platform was used to sequence t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a kit for predicting colorectal cancer liver metastases and a use method. The kit includes a DNA database building kit, the DNA database building kit comprises probes of a plurality of genes, and the plurality of genes include: high risk genes: KRAS, BRAF, MLH1, NRAS, MSH2, PMS2, UGT1A1, MSH6 AKT1, PIK3CA, PTEN, SMAD4, TP53, NM23, TIAM1, MTS1; and low risk genes: PRKDC, RAD50, STAG2, XRCC5, XRCC6, FANCA, ATR, MUTYH, EMSY, ERCC4, RAD51, PARP1, XRCC1. The kit provided by the invention performs related mutation detection on colorectal cancer liver metastases related genes in peripheral blood, and combines specific scoring mechanism to rapidly and conveniently judge and predict colorectal cancer liver metastases.

Description

technical field [0001] The invention relates to a kit and a use method for predicting liver metastasis of colorectal cancer. Background technique [0002] Colorectal cancer, also called colorectal cancer, refers to malignant tumors originating from the colon and rectal mucosa. Pathologically, it refers to colorectal epithelial tumors that penetrate the mucous membrane layer and infiltrate into the submucosa. It is the most common digestive tract tumor One of them is a serious threat to human health. Colorectal cancer is a malignant tumor with the third highest incidence rate and the fourth highest mortality rate in the world, with 1-2 million new cases and 600,000 deaths per year. The incidence and mortality of colorectal cancer in my country are both on the rise. According to the 2014 China Cancer Registration Annual Report, the incidence (16.14 / 100,000) and mortality (7.55 / 100,000) of colorectal cancer rank first among all malignant tumors. Tumor ranks fifth, and most pat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/156
Inventor 刘杜娟徐健唐元华
Owner 苏州首度基因科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap