A method for testing the biotoxicity of oil production wastewater by using Qinghai Vibrio q67

A technology for oil extraction wastewater and Vibrio qinghai, which is applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of not involving wastewater toxicity discharge indicators, etc., and achieves easy popularization and application, stable experimental results, and moderate toxicity. Effect

Active Publication Date: 2018-11-06
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Claims
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Problems solved by technology

The existing "Comprehensive Wastewater Discharge Standard" (GB 8978-1996) stipulates the physical and chemical index limits of wastewater discharge, but does not involve the discharge indicators of wastewater toxicity

Method used

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  • A method for testing the biotoxicity of oil production wastewater by using Qinghai Vibrio q67
  • A method for testing the biotoxicity of oil production wastewater by using Qinghai Vibrio q67
  • A method for testing the biotoxicity of oil production wastewater by using Qinghai Vibrio q67

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Embodiment

[0019] The wastewater samples to be tested in this embodiment are taken from the various treatment process sections of oil production wastewater in a certain petroleum Weizhou terminal treatment plant. The five sampling points are: raw water, ABR pool water, ABR pool effluent, SBR pool water and effluent. The samples were tested as follows:

[0020] (1) Take the oil production wastewater of each process section as the test wastewater and perform pretreatment:

[0021] After the oil production wastewater is transported to the terminal treatment plant through pipelines, it is biochemically treated by the combined process of anaerobic baffle reactor and activated sludge method, and the wastewater treated by biochemical method is transported to the clear water tank and then discharged outside the plant. In order to avoid the influence of turbid wastewater on the experimental results, the sample to be tested was filtered with a 0.45 μm filter membrane and stored in a refrigerator a...

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Abstract

The invention discloses a method for testing the biotoxicity of oil production wastewater by using Qinghai Vibrio Q67. Oil production wastewater was used as the test wastewater, Qinghai Vibrio Q67 was used as the indicator organism, and a 96-well microplate was used as the carrier; through the 96-well microplate analysis method, the luminous intensity was detected by a microplate spectrophotometer; the experimental group was used The luminescence inhibition rate was calculated with the luminescence intensity of the control group, and Zn(NO3)2 was set as the toxicity reference substance, so as to judge the biological toxicity of oil production wastewater. The method of the present invention uses the microplate method to detect and analyze the toxicity of oil production wastewater, and calculates the luminescence inhibition rate of luminescent bacteria to judge the comprehensive toxicity of oil production wastewater, which solves the problem that conventional physical and chemical indicators cannot indicate the biological toxicity of oil production wastewater, and is fast, Sensitive, easy to popularize and apply, and the selected toxic reference substance Zn(NO3)2 has stable experimental results, cheap price and moderate toxicity.

Description

technical field [0001] The invention belongs to the field of wastewater biotoxicity detection, in particular to a method for testing the biotoxicity of oil production wastewater by using Vibrio Q67 of Qinghai. Background technique [0002] Oil production wastewater is the wastewater that needs to be discharged outside during the oil production process of the oil field, except for production water such as reinjection, process remixing or other purposes. It contains high molecular refractory organic pollutants such as petroleum and surfactants. Oil extraction wastewater has the characteristics of high salinity and high water temperature, and is corrosive to objects in contact. In addition, the wastewater also contains a certain amount of fungicides. The existing "Comprehensive Wastewater Discharge Standard" (GB 8978-1996) stipulates the physical and chemical index limits of wastewater discharge, but does not involve the discharge indicators of wastewater toxicity. At present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/64C12Q1/02
CPCC12Q1/02C12Q1/64
Inventor 李艳红肖楠张立浩覃礼堂张鑫宋晓红崔耀宗
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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