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Immune globulin M detection kit and detection method

A technology of immunoglobulin and detection kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low specificity and stability of kits, unstable antibodies, etc., achieve excellent stability, promote binding, good specific effect

Active Publication Date: 2017-05-31
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problems existing in the prior art, the object of the present invention is to provide an immunoglobulin M detection kit, which solves the specificity of the existing kits due to the tendency of magnetic particles to aggregate and the instability of antibodies. and stability issues

Method used

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  • Immune globulin M detection kit and detection method

Examples

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Embodiment 1

[0034] Embodiment 1, a kind of immunoglobulin M detection kit

[0035] The kit described in Example 1 of the present invention includes reagent R1, reagent R2 and immunoglobulin M standard, and said reagent R1 includes: Tris buffer 50mmol / L, accelerator 15mmol / L, polyethylene glycol-400 5g / L L and sodium chloride 20g / L; the reagent R2 includes: Tris buffer 50mmol / L, anti-human immunoglobulin M antibody 20% w / v, sodium chloride 2g / L and stabilizer 5g / L. The accelerator is composed of non-polar amino acid and CHAPS in a weight ratio of 1:0.8, the non-polar amino acid is composed of phenylalanine and leucine in a weight ratio of 1:0.06, and the stabilizer It consists of the following components in parts by weight: 1.2 parts of polyvinylpyrrolidone, 0.06 parts of dithiothreitol, 1.5 parts of inorganic salt, 1 part of bovine serum albumin and 1.5 parts of glycerin.

[0036] Preparation:

[0037]A) Preparation of magnetic particles coated with anti-human immunoglobulin M antibody:...

Embodiment 2

[0040] Embodiment 2, a kind of immunoglobulin M detection kit

[0041] The kit described in Example 2 of the present invention includes reagent R1, reagent R2 and immunoglobulin M standard, and the reagent R1 includes: Tris buffer 20mmol / L, accelerator 5mmol / L, polyethylene glycol-400 0.5g / L and sodium chloride 5g / L; the reagent R2 includes: Tris buffer 20mmol / L, anti-human immunoglobulin M antibody 10%w / v, sodium chloride 5g / L and stabilizer 0.5g / L. The accelerator is composed of non-polar amino acid and CHAPS in a weight ratio of 1:0.5, the non-polar amino acid is composed of phenylalanine and leucine in a weight ratio of 1:0.02, and the stabilizer It consists of the following components in parts by weight: 0.05 part of polyvinylpyrrolidone, 0.01 part of dithiothreitol, 0.1 part of inorganic salt, 0.05 part of bovine serum albumin and 1 part of glycerin.

[0042] The preparation method is as in Example 1.

Embodiment 3

[0043] Embodiment 3, a kind of immunoglobulin M detection kit

[0044] The kit described in Example 3 of the present invention includes reagent R1, reagent R2 and immunoglobulin M standard, and said reagent R1 includes: Tris buffer 300mmol / L, accelerator 50mmol / L, polyethylene glycol-400 10g / L L and sodium chloride 50g / L; the reagent R2 includes: Tris buffer 300mmol / L, anti-human immunoglobulin M antibody 50% w / v, sodium chloride 50g / L and stabilizer 10g / L. The accelerator is composed of non-polar amino acid and CHAPS in a weight ratio of 1:2, the non-polar amino acid is composed of phenylalanine and leucine in a weight ratio of 1:0.1, and the stabilizer It consists of the following components in parts by weight: 2 parts of polyvinylpyrrolidone, 0.06 parts of dithiothreitol, 1.5 parts of inorganic salt, 1 part of bovine serum albumin and 1.5 parts of glycerin.

[0045] The preparation method is as in Example 1.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to an immune globulin M detection kit and detection method. The immune globulin M detection kit comprises a reagent R1, a reagent R2 and an immune globulin M good merchantable quality, the reagent R1 comprises 20-300 mmol / L first buffering solution, 5-50 mmol / L of accelerant, 0.5-10 g / L of polyethylene glycol-400 and 5-50 g / L of sodium chloride; the reagent R2 comprises 20-300 mmol / L of a second buffering solution, 10-50 %w / v of magnetic particles wrapped by anti-human immune globulin M antibodies, 5-50 g / L of sodium chloride and 0.5-10 g / L of stabilizer. Compared with an existing immune globulin M kit, the immune globulin M detection kit has higher specificity, accuracy, precision and stability, and is suitable for large-scale application and popularization.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an immunoglobulin M detection kit and a detection method. Background technique [0002] Immunoglobulins are a group of proteins with antibody activity, mainly found in plasma, but also in other body fluids, tissues and some secretions. Immunoglobulins are divided into five classes, namely Immunoglobulin G (IgG), Immunoglobulin A (IgA), Immunoglobulin M (IgM), Immunoglobulin D (IgD) and Immunoglobulin E (IgE). Among them, IgM is the one with the largest molecular weight among the five classes of immunoglobulins. Its molecular weight is 9×10 5 Dalton, so it is also called macroglobulin. The average concentration in normal human serum is 60-180mg%, accounting for about 6% of the total serum immunoglobulin. Elevation of IgM is common in macroglobulinemia, bacterial and parasitic infectious diseases, liver disease, rheumatoid arthritis, and cystic fibrosis. IgM reduction ...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner ANHUI IPROCOM BIOTECH CO LTD
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