Method for breeding cordyceps militaris by silkworm in early transformation period

A technology of Cordyceps militaris and metamorphosis, applied in botany equipment and methods, cultivation, application, etc., can solve the problems of low efficiency in cultivating Cordyceps militaris, and achieve the effects of increased survival rate of inoculation, less body fluid, and low cultivation cost

Inactive Publication Date: 2017-06-06
北京新创青龙湖种植专业合作社
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for cultivating Cordyceps militaris by silkworms in the early stage of metamorphosis, so as to solve the problem that the efficiency of cultivating Cordyceps militaris with silkworm chrysalis or silkworms is not high

Method used

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Effect test

Embodiment 1

[0020] With the same bacterial classification, the same method selects 50 silkworms and 50 silkworm chrysalis respectively as hosts, and the specific steps of using the silkworms in the early stage of metamorphosis to cultivate Cordyceps militaris are as follows:

[0021] (1) The selection of the host: choose the silkworm chrysalis that has finished the cocoon and does not cast off its skin and become a chrysalis as the host, and choose the mulberry silkworm chrysalis that has just molted and changed the chrysalis as the host;

[0022] (2) Preparation of Cordyceps militaris strains: Preparation of Cordyceps militaris strains: inoculate the slanted mother species of Cordyceps militaris on the slant medium, activate the strains, and scrape off the slant medium after the mycelium turns yellow when exposed to light The mycelium, inoculated in the liquid medium, the liquid medium in the preparation of described Cordyceps militaris bacterial classification comprises the component of ...

Embodiment 2

[0037] With the same bacterial classification, the same method selects 50 tussah silkworms and 50 tussah silkworm chrysalis respectively as hosts, and the specific steps of using the tussah silkworms in the early stage of metamorphosis to cultivate Cordyceps militaris are as follows:

[0038] (1) The selection of the host: select the tussah silkworm that has finished the cocoon and does not cast off its skin and become a chrysalis as the host, and choose the tussah silkworm that has just molted and changed the pupa as the host;

[0039] (2) Preparation of Cordyceps militaris strains: Preparation of Cordyceps militaris strains: inoculate the slanted mother species of Cordyceps militaris on the slant medium, activate the strains, and scrape off the slant medium after the mycelium turns yellow when exposed to light The mycelium, inoculate in the liquid culture medium, the liquid culture medium in the preparation of described Cordyceps militaris bacterial classification comprises t...

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Abstract

The invention relates to a method for breeding cordyceps militaris by silkworm in an early transformation period. According to the method, the living silkworm in the early transformation period serves as a host, cordyceps militaris bacteria are inoculated by an injection method, and inoculation survival rate is greatly increased as the silkworm in the early transformation period is large in body. The concentration of inorganic salt in a strain culture medium is doubled before inoculation, injection is then performed, a more proper inorganic salt environment is provided for growth of the bacteria in silkworm pupas, spawn running time is shortened by 18%, and growth time is shortened by 15.6%. By the aid of the method, the rigidifying rate of the silkworm in the early transformation period reaches 94%-100%, and the fruiting rate of sporocarps reaches 90%-100%. The method for breeding the cordyceps militaris has the advantages of convenience in operation, low breeding rate, good experimental reproducibility and the like, is applicable to large-scale culture and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, and relates to a method for cultivating Cordyceps militaris by using silkworms in the early stage of metamorphosis. Background technique [0002] Cordyceps militaris is also known as Cordyceps militaris. Studies have shown that the types and contents of effective chemical components of Cordyceps militaris are the same or similar to those of Cordyceps sinensis, and they also have the same or similar functions on the human body. They all contain cordycepic acid, cordycepin, polysaccharides, and SO enzymes And ergosterol and other biologically active substances have the effects of improving immunity, anti-tumor, anti-oxidation, anti-fatigue, anti-radiation, lowering blood sugar, lowering blood fat and delaying aging. [0003] In 2009, the Ministry of Health of the People's Republic of China announced No. 3 that Cordyceps militaris was approved as a new resource food, and the consumption amou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01K67/04
CPCA01G18/00A01K67/04
Inventor 石玉梅
Owner 北京新创青龙湖种植专业合作社
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