Construction method of human retinal pigment epithelial cell bank

Abstract

The invention relates to a construction method of a human retinal pigment epithelial cell bank. The method comprises the steps of collecting eyeballs of a donor; separating and obtaining human retinal pigment epithelial cells; carrying out serial subcultivation; carrying out quality detection of the human retinal pigment epithelial cells; carrying out cryopreservation; and constructing the bank. The cryopreserved human retinal pigment epithelial cells revive for clinical injection in use. The method is simple in step and operation; the obtained, cultured and cryopreserved human retinal pigment epithelial cells are high in purity, high in multiplication capacity and high in recovery efficiency; construction of the cell bank is based on HLA matching; the cells subjected to quality detection and HLA matching can be directly applied to clinical injection after reviving; the problems of production, storage and transportation of a cell preparation are solved; and when a patient needs to adopt the human retinal pigment epithelial cells for transporting, the clinically applicable human retinal pigment epithelial cells subjected to HLA matching can be directly provided.

Description

technical field [0001] The invention relates to a method for constructing a human retinal pigment epithelial cell bank, belonging to the field of biotechnology. Background technique [0002] Retinal degenerative diseases are the main cause of irreversible blindness, including age-related macular degeneration, retinitis pigmentosa, and Stargardt disease. Among them, there are about 30 to 50 million patients with age-related macular degeneration worldwide, and about 1 million patients with retinitis pigmentosa. At present, there are more than 20 million patients with age-related macular degeneration in my country, and it is expected to double in 2050. [0003] Since retinal degeneration is mainly manifested as damage to the retinal pigment epithelium (RPE) in the macular area, the replacement transplantation of RPE cells in regenerative medicine has become the main direction of treatment research. So far, RPE cell transplantation from a variety of sources has been reported, ...

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However, no matter which method, the safety of iPSCs before entering the clinic needs to be further verified.

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