Kit for rapidly extracting free nucleic acid in plasma based on paramagnetic particle method

Abstract

The invention discloses a kit for rapidly extracting free nucleic acid from plasma based on a paramagnetic particle method. The kit comprises a lysate, a deproteinization rinsing solution, a desalting rinsing solution, an eluent and magnetic beads, wherein the lysate contains a chaotropic agent with a concentration of 2 to 6 M, 0.05 to 10% of lauryl sodium sulfate, ethylenediamine tetraacetic acid with a concentration of 50 to 200 mM, trihydroxymethyl aminomethane with a concentration of 10 to 200 mM, sodium chloride with a concentration of 50 to 500 mM, 0.1 to 5% of sodium dodecyl benzene sulfonate, 1 to 5% of sodium laureth sulfate and 5 to 15% of a nonionic surfactant. According to the method, unique lysate components and the magnetic beads are adopted, protease K does not need to be added, nucleic acid yield can be effectively increased, and therefore, the yield of downstream NGS library building is increased.

Description

technical field [0001] The invention provides a kit for rapidly extracting plasma free nucleic acid based on a magnetic bead method, which belongs to the field of biomedicine. Background technique [0002] Plasma free nucleic acid refers to the partially degraded endogenous DNA that exists in human plasma and is free outside the cells. Cell-free nucleic acid detection is widely used in non-invasive prenatal screening, tumor diagnosis and various disease testing. [0003] At present, there are many methods for extracting free nucleic acid from plasma in the market. According to the principle of nucleic acid extraction, it can be divided into precipitation method, column membrane centrifugation method, magnetic bead method, etc., and can be divided into manual method and instrument method according to the extraction flux. [0004] Precipitation method: Extract nucleic acid with organic reagents such as phenol chloroform. [0005] Column membrane centrifugation: DNA is bound ...

AI Technical Summary

Problems solved by technology

[0009] Traditional free nucleic acid extraction kits usually use precipitation method and column membrane centrifugation method. The precipitation method needs to use phenol chloroform extraction, which is complicated to operate and highly toxic reagents, so it is not suitable for use

The column membrane method needs to be used with a centrifuge, the operation is complicated, the nucleic acid yield is low, and high-throughput nucleic acid extraction cannot be achieved

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