Cell membrane specific capacitance detection system and method

Abstract

The invention provides a cell membrane specific capacitance detection system comprising a pair of intersect channels, wherein the first channel of the intersect channels is in the first direction, and the second channel is in the second direction intersecting with the first direction; when a cell is driven by a driver and travels a cell travel compressed channel through a cell inflow path, an impedance measuring module records the impedance changes between electrodes, and a cell electricity equivalent model can calculate so as to obtain the cell membrane specific capacitance. In addition, the invention also provides a cell membrane specific capacitance detection method; the detection system can effectively improve the cell membrane specific capacitance detection flux without using optics detection and image analysis, thus effectively reducing cost, simplifying operations, easily carrying out parallelization expansion, and further improving flux on the single channel basis.

Description

technical field [0001] The invention relates to the field of single cell electrical characteristic detection, in particular to a detection system and method for cell membrane specific capacitance. Background technique [0002] Single-cell electrical properties, as an important single-cell biophysical property, have been proven to be used to distinguish different tumor cells and blood cells, and have very important significance and potential value for understanding cell function and state. [0003] In the study of single-cell electrical properties, the cell is equivalent to a capacitor-resistance network, in which the cell membrane with a bilayer phospholipid structure is equivalent to a capacitor, and the cytoplasm mainly composed of electrolytes is equivalent to a resistor. Considering the size heterogeneity of cells, the cell-to-cell comparisons can only be made using parameters such as cell membrane capacitance per unit area that is independent of cell size. [0004] Tra...

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Problems solved by technology

Although this method can obtain the electrical characteristics of single cells with high throughput (about 1 cell / second), it is difficult for the number of cells to reach 10 6 The current method is difficult to meet the needs of single-cell electrical characteristics detection of individual patient tumor samples and so on.

Moreover, this method needs to use a microscope to obtain the length of the cell through an optical method, so as to further obtain the electrical characteristic parameters of the cell independent of the size, so a microscope and a high-speed camera are required, and the process of image and data processing is extremely complicated

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