Prevention method for tetra hybrid bombyx mori pebrine germ vertical transmission

A technology of silkworm microparticles and vertical propagation, which can be applied in the direction of animal husbandry and other directions, and can solve the problems of affecting the yield and quality of silkworm seeds, and the toxic and side effects of silkworms.

Active Publication Date: 2017-08-15
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that long-term use of drugs can cause toxic and side effects to silkworms, affecting the yield and quality of silkworm eggs

Method used

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  • Prevention method for tetra hybrid bombyx mori pebrine germ vertical transmission
  • Prevention method for tetra hybrid bombyx mori pebrine germ vertical transmission

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Effect test

Embodiment 1

[0023] A method for preventing and controlling the vertical transmission of four-element hybrid silkworm microparticle disease embryos, comprising the following steps:

[0024] For the "Liangguang No. 2" quaternary hybrid, 20 hours after laying eggs, the silkworm eggs were immersed in a hydrochloric acid solution with a specific gravity of 1.075 at 47°C for 10 minutes, then taken out, eluted, dried, accelerated greening, and hatched. The practical hatching rate is up to 98%. Microscopic examination of the third instar silkworm shows that the incidence rate is only 7.44%, while the incidence rate in the control group is as high as 83.12%. Compared with the untreated control group, the relative control effect is 91%.

[0025] The formula for calculating the relative control effect is as follows:

[0026] Relative control effect (%)=(100-treatment group morbidity / control group morbidity)×100

[0027] Real-time PCR was used to quantitatively detect the pathogen of Microsporidium ...

Embodiment 2

[0035] A method for preventing and controlling the vertical transmission of four-element hybrid silkworm microparticle disease embryos, comprising the following steps:

[0036] For the "Liangguang No. 2" quaternary hybrid, 20 hours after laying eggs, the silkworm eggs were immersed in a hydrochloric acid solution with a specific gravity of 1.075 at 48°C for 6 minutes, then removed, eluted, dried, accelerated and hatched. The practical hatching rate is up to 96%. Microscopic examination of the third-instar silkworm shows that the incidence rate is only 2.65%, while the incidence rate in the control group is as high as 82.84%. Compared with the untreated control group, the relative control effect is 97%.

[0037] In addition, through Real-time PCR, quantitative detection of the pathogen of No. silkworm in the ant silkworm was carried out, and it was found that the inactivation rate of the pathogen reached 99%.

Embodiment 3

[0039] A method for preventing and controlling the vertical transmission of four-element hybrid silkworm microparticle disease embryos, comprising the following steps:

[0040] For the "Liangguang No. 2" quaternary hybrid, 20 hours after laying eggs, the silkworm eggs were immersed in a hydrochloric acid solution with a specific gravity of 1.075 at 47.5°C for 7 minutes, then removed, eluted, dried, accelerated and hatched. The practical hatching rate is up to 99%. Microscopic examination of the third instar silkworm shows that the incidence rate is only 3.70%, while the incidence rate in the control group is as high as 83.15%, and the relative control effect is 96%.

[0041] In addition, through Real-time PCR, quantitative detection of the pathogen of No. silkworm in the ant silkworm was carried out, and it was found that the inactivation rate of the pathogen reached 97%.

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Abstract

The invention discloses a prevention method for tetra hybrid bombyx mori pebrine germ vertical transmission. The method comprises the following steps: for silkworm eggs which do not need to be subjected to cold storage treatment, enabling the silkworm eggs to stand for 15 to 30h at a temperature of 20 to 30 DEG C after bombyx mori lays the eggs, then carrying out immersion treatment for 6 to 10min in water with a temperature of 47 to 48 DEG C, taking out the silkworm eggs, then eluting the silkworm eggs with clear water stage by stage until the silkworm eggs are odorless after are, and carrying out hatching hastening after drying; for silkworm eggs subjected to cold storage treatment, taking the silkworm eggs out of a cold storage, protecting for hours at a temperature of 10 to 20 DEG C, carrying out immersion treatment in water with a temperature of 47 to 52 DEG C for 6 to 10min, taking out the silkworm eggs, then eluting the silkworm eggs with clear water stage by stage until the silkworm eggs are odorless, drying, and then carrying out hatching hastening. The method disclosed by the invention can inactivate 97 to 99% of tetra hybrid bombyx mori sporozoan pathogeny, reaches a relative control effect of 90 to 97% on bombyx mori pebrine germ vertical transmission, can reach silkworm egg hatchability of over 93%, and has no harmful effect on growth development of bombyx mori.

Description

technical field [0001] The invention belongs to the field of sericulture production, and in particular relates to a method for preventing and controlling the vertical transmission of microparticle disease embryos of four-element hybrid silkworm silkworm. Background technique [0002] Bombyx mori microparticle disease is the most serious devastating infectious disease in sericulture production. The pathogen of the disease is Nosema bombycis, which is a typical species of Nosema genus. Microsporidium silkworm can parasitize in silkworm midgut, silk gland, muscle, Malpighian duct and other tissues. After infection, the silkworm is inactive, stunted, and dark brown spots appear on the body surface, eventually leading to the death of the silkworm. [0003] Microsporidium silkworm can not only infect silkworms by eating mulberry leaves, but also can be transmitted through silkworm eggs. Therefore, the disease has always been valued by the industry, and it has been listed as the on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 邢东旭杨琼廖森泰李庆荣肖阳赵超艺叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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