Method for establishing DNA molecular tags of Yunjing series rice varieties

Abstract

The invention discloses a method for establishing DNA molecular tags of Yunjing series rice varieties. According to the method, 6 pairs of specific primers for identifying the Yunjing series rice varieties are provided, the Yunjing series rice varieties are identified with fewest primers at the wide genome coverage degree, DNA molecular tags of all the Yunjing series rice varieties can be constructed with fewest primers, the heavy workload and the high detection cost are reduced, the Yunjing series rice varieties can be distinguished from other Yunnan plateau japonica rice varieties, and meanwhile, the Yunjing series rice varieties can be distinguished one by one. According to the method, DNA fingerprint data, obtained through amplification, of the Yunjing series rice varieties and acquired basic commodity information are subjected to digital coding assignment to form 2-dimensional code expression, the DNA molecular tags of Yunjing series rice varieties can be obtained, uniqueness, identifiability and traceability of variety identification and scientific seed management can be realized, and the DNA molecular tags are marked on commercial seed packaging and used for faking prevention and tracing of good rice variety seeds.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to the establishment of a DNA molecular tag of Yunjaponica series rice varieties, which is applied to variety molecular identification and variety management. Background technique [0002] Yunnan's unique natural environment has created conditions for the formation of rich rice resources. At the same time, diverse ethnic cultures have continuously promoted the enrichment and development of rice resources, making Yunnan the genetic diversity center of China's cultivated rice. One of the two provinces with wild rice resources. Abundant rice resources have bred a large number of high-quality, high-yield, and stress-resistant varieties, which have rich diversity and plateau characteristics. [0003] The Yunjing series rice varieties are a series of high-yield and high-quality rice varieties selected by the Japonica Rice Breeding Innovation Team of the Yunnan Acade...

AI Technical Summary

Problems solved by technology

Yunjing series rice varieties (lines) have the characteristics of "high yield, high quality, disease resistance, and lodging resistance". They have been widely promoted and applied in Yunnan Province and surrounding provinces. The phenomenon of "chaotic and miscellaneous" and "homogenization of varieties" coexist, and the phenomenon of "same species with different names", "same name with different species" and "counterfeit deck" have been repeatedly banned, and incidents of counterfeiting and selling counterfeit goods have occurred from time to time, which has affected the interests of the country and farmers. Great damage, bringing many difficulties to variety management and property rights protection

Images