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Breeding method of chahua chicken

A camellia chicken and protein technology, applied in chemical instruments and methods, anti-animal/human immunoglobulin, material inspection products, etc., can solve the problems of long breeding cycle and achieve the effect of shortening the breeding cycle

Active Publication Date: 2017-08-18
INST OF ANIMAL HUSBANDRY & VETERINARY SCI SHANXI ACAD OF AGRI SCI SAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of shape selection has a longer breeding cycle

Method used

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  • Breeding method of chahua chicken
  • Breeding method of chahua chicken
  • Breeding method of chahua chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Analysis of the Conserved Sequence of Foxp2 in Dalan Chicken

[0034] (1) Amplification of partial cDNA of Foxp2 gene coding region

[0035] The RNA extracted from the brain of the newborn Dalan chicken was reverse-transcribed into cDNA, and then PCR amplification was carried out with primers designed in the conserved region of Foxp2 and the cDNA as a template. The primer sequences are shown in SEQ ID NO: 1-4.

[0036] Table 1 Primer sequences

[0037]

[0038] (2) RACE process

[0039] Refer to the description of the GeneRacer kit, the main principle is: first dephosphorylate, remove the cap, and connect the RNA Oligo provided by the kit to the 5' end of the total RNA or mRNA, and then use the poly(T)18-containing poly(T)18 provided by the kit to Adapter primers for reverse transcription.

[0040] (3) Use the gene-specific primers designed according to the cDNA sequence of the partial coding region of the gene and the linker universal primer provided b...

Embodiment 2

[0048] Example 2 Conserved Sequence Analysis of Camellia Chicken Foxp2

[0049] Referring to the test method in Example 1, the conserved sequence of Camellia chicken Foxp2 was obtained, as shown in SEQ ID NO:14. After detection, the sequence is 51bp more base than that shown in SEQ ID NO: 13. The results of the alignment are shown in figure 1 .

Embodiment 3

[0050] Example 3 Preparation of polyclonal antibody

[0051] According to the results of RACE in Example 2, after predicting the FoxP2 protein epitope, design a protein antibody that can only recognize more than 51bp (the antibody corresponding to the protein sequence shown in SEQ ID NO: 14), after predicting the FoxP2 protein epitope, select the open read The 135th-145th amino acid sequence CLQEFYKKQQEQ of the code frame (Note: C is added at the end is for carrier coupling), its sequence is shown in SEQ ID NO: 15, and a peptide was synthesized to prepare a polyclonal antibody. Specific steps are as follows:

[0052] 1) Polypeptide synthesis: artificially synthesized polypeptide (10-14mg, purity above 85%);

[0053] 2) Carrier coupling: the synthetic polypeptide-coupling protein carrier pEGFP-N1 carrier;

[0054] 3) Animal immunization, antiserum preparation: immunize 2 New Zealand white rabbits (initial immunization plus 3-4 booster immunizations); pre-collection of blood; da...

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Abstract

The invention relates to the technical field of poultry breeding, and in particular to a breeding method of chahua chicken. The breeding method of the chahua chicken comprises the following steps: extracting striatal total protein of chicken's brain tissue, and carrying out electrophoresis, thus obtaining a separated protein sample; transferring the separated protein sample to a solid-phase carrier; carrying out immunoreaction on a polyclonal antibody prepared by the invention and the protein on the solid-phase carrier by adopting a Western blotting method, and detecting an antigen-antibody complex. The antibody prepared by the invention is capable of recognizing an immunogen, and the titer is greater than 1 to 20,000. According to the breeding method of the chahua chicken, provided by the invention, the chahua chicken can be accurately selected, the selection is not confined to shape any more, early breeding can be carried out, and the breeding period is shortened.

Description

technical field [0001] The invention relates to the technical field of poultry breeding, in particular to a method for breeding camellia chickens. Background technique [0002] Camellia Chicken (English name: Chahua Chickens) is commonly known as jungle chicken, red jungle chicken, pheasant, and candle night. Famous in Southwest China, it is produced in the deep mountains and old forests around Xishuangbanna. It is bred from wild red junglefowl after long-term domestication and selection. It is a rare original chicken breed in China. Because of its crowing sound like "two camellias", it is named Camellia chicken. It has the characteristics of strong adaptability, extensive breeding, low feed intake and high survival rate. It is a unique rare bird species in China. With the strengthening of people's awareness of protection, Camellia chicken, like other wild animals, has been protected in Xishuangbanna Nature Reserve. In the future, further research on the biological charact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C07K16/18C07K16/06
CPCC07K16/065C07K16/18G01N33/68
Inventor 王曦张峰靳光李博温永亮韩洁茹赵鹏任宏铮田歌侯志宏张荣华
Owner INST OF ANIMAL HUSBANDRY & VETERINARY SCI SHANXI ACAD OF AGRI SCI SAAS
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