Method for constructing half adder and half subtracter based on interaction between cyanine dye and metal ions

Abstract

The invention provides a method for constructing a half adder and a half subtracter based on the interaction between a cyanine dye and metal ions, and the method is characterized by comprising the steps: a cyanine dye solution and DNA double strands are taken as templates, Tris-HCl buffer solutions with different pH values and metal ion solutions are taken as two parallel paths of input signals, the DNA double strands are induced into four-strand structures under regulation and control of the Tris-HCl buffer solutions and the metal ions, the cyanine dye interacts with the DNA four-strand structure through non-covalent bond acting force to generate spectral signals, and the ultraviolet absorption values and / or fluorescence intensities of the cyanine dye are taken as output signals to construct the half adder or half subtracter. The half adder or half subtracter constructed by the scheme has good molecular logic circuit property, high sensitivity, large on off ratio, easy differentiation and low threshold of 0.3. The detection of the output signals is easy and can be realized by an absorption / fluorescence spectra instrument, and the ion concentration of a solution can be detected efficiently, sensitively, simply and rapidly.

Description

technical field

[0001] The invention belongs to the technical field of molecular computers, and in particular relates to a method for constructing half-adders and half-subtractors based on the interaction between cyanine dyes and metal ions. Background technique

[0002] A logic circuit is a discrete signal transmission and processing circuit based on the principle of binary to realize logical operations and operations of digital signals. Logic gates are the basic elements of digital logic circuits, which can be composed of transistors. The combination of these transistors can make the high and low levels representing the two signals pass through them to generate a high or low level signal. High and low levels can respectively represent 1 and 0 in binary, thus realizing logic operations. Logic gates can be used in combination to implement more complex logic operations, such as logic circuits such as half adders and half subtractors. They are the basis of the arithmetic lo...

Examples

Embodiment 1

[0032] (1) Build a half adder

[0033] Take four EP tubes, numbered 1, 2, 3 and 4, and do the following:

[0034] Add 30 μL to tube 1 with a concentration of 50×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 100×10 -6 mol / L cyanine dye I solution, finally add 5×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 1;

[0035] Add 30 μL to the No. 2 tube with a concentration of 50×10 -6 mol / L DNA double strand and 10 μL KCl solution with a concentration of 0.5 mol / L, and then add 20 μL with a concentration of 100×10 -6 mol / L cyanine dye I solution, finally add 5×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 2;

[0036] Add 30 μL to the No. 3 tube with a concentration of 50×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 100×10 -6 mol / L cyanine dye I solution, finally add 5×10 -3 mol / L, Tris-HCl buffer solution with pH=4.0, make the solution volume ...

Embodiment 2

[0075] (1) Build a half adder

[0076] Take four EP tubes, numbered 1, 2, 3 and 4, and do the following:

[0077] Add 30 μL to the No. 1 tube with a concentration of 100×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 200×10 -6 mol / L cyanine dye III solution, finally add 10×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 1;

[0078] Add 30 μL to tube 2 with a concentration of 100×10 -6 mol / L DNA double strand and 20 μL KCl solution with a concentration of 1.0 mol / L, and then add 20 μL with a concentration of 200×10 -6 mol / L cyanine dye III solution, finally add 10×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 2;

[0079] Add 30 μL to tube 3 with a concentration of 100×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 200×10 -6 mol / L cyanine dye III solution, finally add 10×10 -3 mol / L, Tris-HCl buffer solution with pH=4.0, make the solution vol...

technical field

[0001] The invention belongs to the technical field of molecular computers, and in particular relates to a method for constructing half-adders and half-subtractors based on the interaction between cyanine dyes and metal ions. Background technique

[0002] A logic circuit is a discrete signal transmission and processing circuit based on the principle of binary to realize logical operations and operations of digital signals. Logic gates are the basic elements of digital logic circuits, which can be composed of transistors. The combination of these transistors can make the high and low levels representing the two signals pass through them to generate a high or low level signal. High and low levels can respectively represent 1 and 0 in binary, thus realizing logic operations. Logic gates can be used in combination to implement more complex logic operations, such as logic circuits such as half adders and half subtractors. They are the basis of the arithmetic lo...

Examples

Embodiment 1

[0032] (1) Build a half adder

[0033] Take four EP tubes, numbered 1, 2, 3 and 4, and do the following:

[0034] Add 30 μL to tube 1 with a concentration of 50×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 100×10 -6 mol / L cyanine dye I solution, finally add 5×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 1;

[0035] Add 30 μL to the No. 2 tube with a concentration of 50×10 -6 mol / L DNA double strand and 10 μL KCl solution with a concentration of 0.5 mol / L, and then add 20 μL with a concentration of 100×10 -6 mol / L cyanine dye I solution, finally add 5×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 2;

[0036] Add 30 μL to the No. 3 tube with a concentration of 50×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 100×10 -6 mol / L cyanine dye I solution, finally add 5×10 -3 mol / L, Tris-HCl buffer solution with pH=4.0, make the solution volume ...

Embodiment 2

[0075] (1) Build a half adder

[0076] Take four EP tubes, numbered 1, 2, 3 and 4, and do the following:

[0077] Add 30 μL to the No. 1 tube with a concentration of 100×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 200×10 -6 mol / L cyanine dye III solution, finally add 10×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 1;

[0078] Add 30 μL to tube 2 with a concentration of 100×10 -6 mol / L DNA double strand and 20 μL KCl solution with a concentration of 1.0 mol / L, and then add 20 μL with a concentration of 200×10 -6 mol / L cyanine dye III solution, finally add 10×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5, make the solution volume 1mL to obtain solution 2;

[0079] Add 30 μL to tube 3 with a concentration of 100×10 -6 mol / L DNA duplex, and then add 20 μL concentration of 200×10 -6 mol / L cyanine dye III solution, finally add 10×10 -3 mol / L, Tris-HCl buffer solution with pH=4.0, make the solution vol...

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