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Method for preparing natural protein compound micelle with disulfide bond reconstruction method

A technology of composite micelles and natural proteins, which is applied in the field of preparing natural protein composite micelles by disulfide bond reconstruction method, can solve the problems of slow metabolism rate, limited function and accumulation, and achieves good biocompatibility and metabolizable products. , the effect of low price

Active Publication Date: 2017-09-01
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved in the present invention is that the amphiphilic block copolymers of artificially synthesized polymer micelles have a slow metabolic rate in organisms and will accumulate. Toxicity: A certain natural polymer protein is used as a single application micelle, the shape is uncontrollable, the particle size and distribution are not uniform, and the function is limited. It provides a safe, non-toxic, stable structure, good biocompatibility, metabolizable products, and low price. Method for preparing natural protein complex micelles by the disulfide bond reconstruction method

Method used

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  • Method for preparing natural protein compound micelle with disulfide bond reconstruction method
  • Method for preparing natural protein compound micelle with disulfide bond reconstruction method
  • Method for preparing natural protein compound micelle with disulfide bond reconstruction method

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Embodiment 1

[0026] A method for preparing natural protein complex micelles by a disulfide bond reconstruction method, comprising the steps of:

[0027] ① Preparation of mass concentration of 0.5% keratin solution; keratin through Na 2 Prepared by S reduction and acid precipitation method, the method is as follows: a. Combine degreased and washed wool or animal hoof horn powder with Na 2 S is added to a closed glass container according to the mass ratio of 4:3, water with 20 times the mass of wool or hoof horn powder is added, and the keratin solution is obtained by placing it in the dark for 10 days; b. Add concentrated hydrochloric acid to the keratin solution within 20 seconds to make The pH value of the keratin solution reaches 2.0-2.5 within 30 seconds, causing the keratin to precipitate rapidly; c. After the keratin is precipitated, the keratin precipitate in the lower layer is collected, washed twice with anaerobic water, and vacuum-dried at 5°C to obtain white keratin Protein soli...

Embodiment 2

[0031] A method for preparing natural protein complex micelles by a disulfide bond reconstruction method, comprising the steps of:

[0032] ① Preparation of mass concentration of 5% keratin solution; keratin through Na 2 Prepared by S reduction and acid precipitation method, the method is as follows: a. Combine degreased and washed wool or animal hoof horn powder with Na 2 S is added to a closed glass container according to the mass ratio of 4:3, water with 20 times the mass of wool or hoof horn powder is added, and the keratin solution is obtained by keeping it in the dark for 7-10 days; b. Add concentrated hydrochloric acid to the keratin solution within 20 seconds , so that the pH value of the keratin solution reaches 2.0-2.5 within 60 seconds, so that the keratin precipitates rapidly; c. After the keratin precipitates, the keratin precipitate in the lower layer is collected, washed 4 times with anaerobic water, and vacuum-dried at 20°C to obtain White keratin solid powder...

Embodiment 3

[0036] A method for preparing natural protein complex micelles by a disulfide bond reconstruction method, comprising the steps of:

[0037] ① Preparation of mass concentration of 2.5% keratin solution; keratin through Na 2 Prepared by S reduction and acid precipitation method, the method is as follows: a. Combine degreased and washed wool or animal hoof horn powder with Na 2 S is added into a closed glass container according to the mass ratio of 4:3, water with 18 times the weight of wool or hoof horn powder is added, and the keratin solution is obtained by placing it in the dark for 8 days; b. Add concentrated hydrochloric acid in the keratin solution within 20 seconds to make The pH value of the keratin solution reaches 2.0-2.5 within 40 seconds, causing the keratin to precipitate rapidly; c. After the keratin is precipitated, the keratin precipitate in the lower layer is collected, washed 3 times with anaerobic water, and vacuum-dried at 20°C to obtain white keratin Protei...

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Abstract

The invention discloses a method for preparing a natural protein compound micelle with a disulfide bond reconstruction method, and aims to solve the technical problems that the amphiphilic block copolymer of an artificially synthesized polymeric micelle has a low metabolic rate in a living body and can accumulate and parts of amphiphilic block copolymer have certain toxicity for the living body. The method comprises the following steps: preparing a keratin solution; preparing a natural macromolecule protein solution; preparing the compound micelle with the disulfide bond reconstruction method: a: mixing the keratin solution prepared in 1) with the natural macromolecule protein solution prepared in 2) to obtain a protein mixed solution; b: carrying out the quantitative reconstruction of a disulfide bond: putting the mixed solution of two types of protein in a constant temperature of 15-25 DEG C, standing for 3-12h, and measuring the specific numerical value of a sulfhydryl content in the mixed solution; c: calculating a quantity of an oxidizing agent which needs to be added, and obtaining the protein compound micelle after the oxidizing agent is added. The method which adopts the technical scheme has the outstanding advantages of safety, no toxin, stable structure, good biocompatibility, metabolizable product, low price and the like.

Description

technical field [0001] The invention relates to a method for preparing natural protein composite micelles, in particular to a method for preparing natural protein composite micelles by adopting a disulfide bond reconstruction method. Background technique [0002] Amphiphilic block copolymers are a class of polymers containing both hydrophilic groups and hydrophobic groups, which can spontaneously form a high-density structure consisting of a hydrophobic core and a hydrophilic shell through self-assembly in a selective solvent. Molecular micelles. Due to its unique structural characteristics and potential application properties, amphiphilic block copolymers have increasingly become a research hotspot in the field of polymer science. The prepared micelles have broad application prospects in the fields of medicine, biology, chemical industry, and materials. [0003] Amphiphilic block copolymer systems include synthetic polymers and natural polymer systems. At present, most of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08L89/00
CPCC08J3/075C08J3/24C08J2389/00C08L89/00C08L2205/025
Inventor 苏小舟栗蕾
Owner ZHONGYUAN ENGINEERING COLLEGE
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