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Bi-enzymatic hydrolysis method for preparing high-F-value oligopeptide crude liquid

A double-enzyme hydrolysis and oligopeptide technology, applied in the direction of fermentation, can solve the problems of unsatisfactory protein conversion rate and F value to be improved, and achieve the effects of shortening preparation time, obvious deodorizing effect and improving taste.

Inactive Publication Date: 2017-09-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein conversion rate after enzymatic hydrolysis is not ideal, and the F value needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Double enzymatic hydrolysis method to prepare high F value oligopeptide crude solution, the specific steps are:

[0019] 1) Pretreatment: Remove the head, skin and viscera of the hairtail, rinse it with clean water, and cut it into small pieces;

[0020] 2) To remove fishy smell: add fishy smelly liquid to the hairtail treated in step 1 at a volume ratio of 1:4~8, the ingredients and parts by weight of the fishy fishy liquid are: 5~10 parts of citric acid, 10~18 parts of perilla extract 0.1~0.3 parts of sodium chloride, 0.5~0.8 parts of sodium bicarbonate, 0.001~0.003 parts of ursolic acid, 20~30 parts of ethanol, 0.02~0.04 parts of kaempferin and 0.2~0.4 parts of vitamin E. Soak, the soaking time is 4~8h. The characteristic component of the fishy smell substance on the hairtail is trimethylamine. The above-mentioned fishy smell removal liquid can not only react with trimethylamine to form a quaternary ammonium salt, but also dissolve trimethylamine, and the fishy smel...

Embodiment 2

[0024] Double enzymatic hydrolysis method to prepare high F value oligopeptide crude solution, the most preferred steps are:

[0025] 1) Pretreatment: Remove the head, skin and viscera of the hairtail, rinse it with clean water, and cut it into 4cm long pieces;

[0026] 2) Deodorization: Add deodorization solution to the hairtail treated in step 1 at a volume ratio of 1:6. The components of the deodorization solution and their most preferred parts by weight are: 8 parts of citric acid, 17 parts of perilla extract, 0.1 part of Sodium chloride, 0.5 part of sodium bicarbonate, 0.001 part of ursolic acid, 30 parts of ethanol, 0.04 part of kaempferin and 0.3 part of vitamin E. Soak, the soaking time is 7h. The characteristic component of the fishy smell substance on the hairtail is trimethylamine. The above-mentioned fishy smell removal liquid can not only react with trimethylamine to form a quaternary ammonium salt, but also dissolve trimethylamine, and the fishy smell removal ef...

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Abstract

The invention provides a bi-enzymatic hydrolysis method for preparing high-F-value oligopeptide crude liquid. The method includes steps: 1) pretreatment; 2) deodorization; 3) degreasing; 4) enzymolysis. The method has advantages that a pepsase enzyme activator and a flavourzyme enzyme activator are capable of activating pepsase and flavourzyme respectively to improve enzymolysis efficiency and shorten oligopeptide crude liquid preparation time; the pepsase which belongs to aspartic protease is inclined to shear an amino terminal or carboxyl terminal into aromatic amino acid, and aromatic amino acid residue exposure rate is high, so that exonuclease specific excision at the second step is facilitated; the flavourzyme is natural and safe and is exactly capable of serving as exonuclease to degrade bitter peptides obtained at first-step enzymolysis into amino acids. In addition, by terminal peptide hydrolysis, hydrolysis degree is raised.

Description

technical field [0001] The invention relates to the technical field of preparation of active peptides, in particular to a double-enzyme hydrolysis method for preparing crude liquid of oligopeptides with high F value. Background technique [0002] Hairtail (Largehead Hairtail), also known as saury and octopus, belongs to the phylum Chordate and the order Perciformes, with a ribbon-shaped body. It is mainly distributed in the Western Pacific Ocean and the Indian Ocean. It is distributed in the Yellow Sea, East China Sea, Bohai Sea and the South China Sea. It is also known as China's four major seafood along with large yellow croaker, small yellow croaker and squid. [0003] In 2014, the catch of hairtail in Zhoushan City reached 108,104 tons, accounting for 11.52% of the main caught species. Zhoushan hairtail is mostly sold fresh or processed into various fish products. However, the development and utilization of hairtail is still in the rough processing stage, and the utiliz...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 王斌赵文浩赵玉勤丁冬各
Owner ZHEJIANG OCEAN UNIV
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