The invention discloses a production method and applications of a novel
rhizomucor miehei aspartic
protease, and particularly relates to a recombinant aspartic
protease, a coding
gene, and a production method and applications of the recombinant aspartic
protease. The recombinant aspartic protease is
protein having an
amino acid sequence shown as SEQ ID NO:1. The coding
gene of the recombinant aspartic protease is
a DNA molecule shown as SEQ ID NO:2. An aspartic
protease gene that is RmproA of
rhizomucor miehei is connected to a
pichia pastoris GS115
expression vector pPIC9K, and is converted into
pichia pastoris and induced expression is performed to obtain the recombinant aspartic protease. Through high-density
fermentation in a
fermentation tank having a volume of 5 L, the maximum protease production activity at 156 h of a recombinant strain is 3400 U / mL, and the
protein content is 6.42 mg / mL. The recombinant aspartic protease is capable of effectively tendering pork, reducing shearing force, making meat palatable, and effectively hydrolyzing animal and
plant protein to prepare low-molecular-weight polypeptides.