Micro-molecular chip, its preparation method and application

A technology for small molecules and small molecular compounds, which is applied in the analysis of materials, material analysis by optical means, material inspection products, etc. question

Active Publication Date: 2017-09-26
苏州普芯生命科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these existing detection methods are complicated to operate, take a long time to detect, and have a large workload, which is not conducive to the prevention, timely diagnosis and control of diseases. Therefore, a new detection method is urgently needed, and the use of small molecule chips to detect biological samples to be tested The presence or absence of pathogens is undoubtedly one of the ideal detection methods
There are no related reports on small molecule chips

Method used

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  • Micro-molecular chip, its preparation method and application
  • Micro-molecular chip, its preparation method and application
  • Micro-molecular chip, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0164] Example 1 Screening of Small Molecular Compounds

[0165] The chip used in this example is a two-dimensional photocrosslinking chip, which includes a solid support, polyethylene glycol modified at both ends and a photocrosslinking agent; the polyethylene glycol modified at both ends is modified by a riveting group at one end, The other end is polyethylene glycol modified by a binding group, the solid support is connected to the riveting group, and the photocrosslinking agent is connected to the binding group; the riveting group is -SH, and the binding group is -COOH 3 , its preparation method is:

[0166] Step 1: Take polyethylene glycol and connect it with the riveting group and the binding group respectively to obtain polyethylene glycol modified at both ends;

[0167] Step 2: Take the solid support and connect it with the riveting group after pretreatment;

[0168] Step 3: After activating the binding group, it is coupled with the photocrosslinking agent through ac...

Embodiment 2

[0228] Example 2 Preparation of Small Molecule Chip

[0229] The chip used in this example is a two-dimensional photocrosslinking chip, which includes a solid support, polyethylene glycol modified at both ends and a photocrosslinking agent; the polyethylene glycol modified at both ends is modified by a riveting group at one end, The other end is polyethylene glycol modified by a binding group, the solid support is connected to the binding group, and the photocrosslinking agent is connected to the binding group; the binding group is -S-S-, and the binding group is -OH.

[0230] Take the following small molecule compounds:

[0231] (8R,9S,13S,14S,17R)-13-Methyl-17-ethyl-17-hydroxy-1,2,4,6,7,8,9,11,12,14,15,16- Dodecylcyclopentanol[1]phenylalanine-3-one;

[0232] (2S,5R,6R)-6-[[3-(2-Chloro-6-fluorophenyl)-5-methyl-1,2-oxazole-4-formyl]amino]-3,3- Dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid sodium salt;

[0233] 3-(3-Ethylhexahydro-1-methyl-1H-azepine-3-...

Embodiment 3

[0246] Example 3 Preparation of Small Molecule Chip

[0247] The chip used in this embodiment is a three-dimensional photocrosslinking chip, which includes a solid support, an initiation composition, an initiation monomer and a photocrosslinking agent; the initiation monomer is connected to the solid support through the initiation composition, and the photocrosslinking agent and the initiation The monomer is connected; the initiating composition includes an initiator and a diluent; the initiator is a dithiol initiator. The preparation method of the chip comprises the following steps:

[0248] Step 1: mixing the initiator and the diluent to obtain the initiator composition;

[0249] Step 2: After taking the solid support and pretreating it, the polymerization of the monomer is initiated by the initiation composition to obtain a high molecular weight polymer;

[0250] Step 3: activate the high molecular polymer, and couple with the photocrosslinking agent through acid amine co...

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Abstract

The invention belongs to the technical field of a biological chip, and discloses a micro-molecular chip, its preparation method and an application. The micro-molecular chip comprises a chip and a micro-molecular compound; the chip comprises light crosslinking agent, and the micro-molecular compound is connected with the chip through the light crosslinking agent. The micro-molecular compound in the micro-molecular chip can be combined with plasmodium aspartic protease II protein or hemagglutinin protein, and used for detecting the plasmodium aspartic protease II protein and / or hemagglutinin protein in a to-be-detected sample; the micro-molecular chip has advantages of being simple in operation during detection process, exact in detection result, good in reproducibility, short in time, time-saving and labor-saving.

Description

technical field [0001] The invention belongs to the technical field of biochips, and in particular relates to a small molecule chip, its preparation method and application. Background technique [0002] In the early stage of the development of biochips, the main research object was DNA chips. After years of development, the scope of biochips has gone far beyond the DNA chips themselves. At present, there are many classification methods of biochips. According to the structural characteristics of biochips, biochips can be divided into microarray chips and microfluidic chips. Microarray chips mainly include small molecule chips, DNA chips, protein chips, cell chips, and tissue chips. The molecules immobilized on these chips are small molecules, DNA, proteins, etc. Their working principles are all based on the affinity binding between biomolecules, for example, the base pairing of nucleic acid molecules, the binding of antigens and antibodies, etc. [0003] The information ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/552
CPCG01N21/553G01N33/68Y02A50/30
Inventor 赵智张振伟何建安彭开美祁婷婷朱劲松
Owner 苏州普芯生命科学技术有限公司
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