Specific promoter of soybean seeds and use thereof

A soybean seed-specific technology, applied in the field of plant genetic engineering, can solve problems such as the lack of soybean seed-specific promoters
CN101818151AInactive Publication Date: 2010-09-01JILIN UNIV
1 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JILIN UNIV
Publication Date
2010-09-01
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a specific promoter of soybean seeds, which belongs to the sequence and use of a soybean aspartic protease gene promoter. The nucleotide sequence of the promoter is represented by the sequence No.1. The preparation method of the promoter comprises the following steps of: cloning the upstream distal sequence of gene soyAP1, analyzing the upstream sequence of the ATG of the gene soyAP1 and determining the deleted fragments SAP1, SAP2, soyAP1-p and SAP4; and amplifying the soyAP1-p fragment, linking the amplified fragment of the soyAP1-p with a cloning vector pMD 18-T, performing identification, measuring the sequence, and verifying the sequence. In the invention, the soyAP1-p promoter of soybean is cloned and expressed in the soybean seeds specifically, and the promoter obtained can be used as a favorable tool in researches on soybean transgenosis and particularly creates conditions for researching and developing soybean seed bioreactors.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of plant genetic engineering, in particular to the sequence of soybean aspartic acid protease gene promoter and its application. Background technique

[0002] Soybean is an important economic crop in my country. The grain is rich in protein, lipid and various nutrients. The phosphorus, iron and calcium minerals are dozens of times higher than other crops, and contain many vitamins, especially soybean contains The 8 essential amino acids synthesized are unmatched by other cereal crops. Soybean is not only the main source of protein and lipids for human beings, but also has a very obvious role in health care. Therefore, using soybean seeds as bioreactors and using plant genetic engineering methods to produce new soybean varieties with industrial uses and medicinal values ​​from transgenic soybean seeds has become a research hotspot.

[0003] To increase or decrease the content of certain substances in transgenic soybea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More