High-throughput, high-content and parallel imaging device and screening system for biological cell chips

Abstract

The invention discloses a high-throughput, high-content and parallel imaging device and a screening system for biological cell chips. The high-throughput, high-content and parallel imaging device comprises a sample table, a light source assembly, a primary imaging assembly, a secondary imaging assembly and a detector. First imaging is integrally completely carried out on cell island array regions of the biological cell chips in first imaging planes by the primary imaging assembly in optical axis sharing imaging modes, and first formed images on the first imaging planes comprise first hole effective regions which are separately arranged in array forms; second imaging is completely carried out on the various first hole effective regions on detection planes of the detector by the secondary imaging assembly in light path splitting imaging modes, second formed images on the detection planes of the detector comprise second hole effective regions which are separately arranged in array forms, and gaps between every two adjacent second hole effective regions are determined by secondary preset amplification factors. The high-throughput, high-content and parallel imaging device and the screening system have the advantages that gapless imaging can be carried out on the various hole effective regions of the biological cell chips, the utilization rate of camera pixels can be maximized, and accordingly high-throughput, high-content and parallel imaging and screening can be carried out.

Description

technical field [0001] The invention relates to the field of imaging technology, in particular to a high-throughput, high-content, parallel imaging device and screening system for a biological cell chip. Background technique [0002] Explanation of terms: "High throughput" corresponds to "large field of view", "high content" corresponds to "high resolution", and "parallel" corresponds to "multi-hole synchronous" imaging. [0003] High-throughput and high-content screening technology refers to the simultaneous detection of the influence of samples on various aspects of cell morphology, growth, apoptosis, metabolic pathways and signal transduction under the premise of maintaining the integrity of cell structure and function, and to determine its biological activity and Potentially toxic. Compared with molecular-level screening such as proteins, cell-level screening eliminates the need for purification of the target protein, making the conformation and environment of the targe...

AI Technical Summary

Problems solved by technology

[0009] (2) There is no parallel comparability between kinetic experiment samples

Through multiple scans and image stitching in each well, there is a significant time difference between wells (ranging from ten minutes to several hours), and it is impossible to analyze the kinetic changes of the sample (such as the drug triggering of the calcium ion signal of the cell) changes, dynamic changes in mitochondrial membrane potential) for simultaneous parallel observation

[0010] (3) Resolution and sample parallelism cannot be taken into consideration. It is necessary to reduce the resolution and increase the imaging speed of each field of view to reduce the time difference between holes, or sacrifice the comparability and time difference of imaging between holes to improve resolution.

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