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Free DNA preservation liquid and preparation method and application thereof

A storage solution and buffer technology, applied in the field of cell-free DNA storage, to effectively inhibit bacterial reproduction, prevent cell-free DNA from being degraded, and apply to a wide range of samples

Pending Publication Date: 2017-10-20
BEIJING ABACE BIOTECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The present invention aims to solve the problems described above

Method used

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  • Free DNA preservation liquid and preparation method and application thereof
  • Free DNA preservation liquid and preparation method and application thereof
  • Free DNA preservation liquid and preparation method and application thereof

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preparation example Construction

[0055] The present invention also provides a method for preparing a free DNA preservation solution, comprising the following steps: placing a predetermined content of disodium edetate, a pH stabilizer, imidazolidinyl urea, and glycine in a beaker, and placing them in a water bath at 60-65°C for 25 ~35min, preferably, 65°C water bath for 30min; after dissolving, add a predetermined amount of Triton X-100, and finally add deionized water to make up to 100mL, mix evenly; 0.25μm membrane filter to obtain free DNA preservation solution. The pH value of the obtained preservation solution is 5.5-7.0.

[0056] The free DNA preservation solution of the present invention is applied to the preservation of free DNA in urine and tissue fluid. The ratio of the preservation solution to the preservation sample is 1:20-1:10.

[0057] Example preservation solution preparation

[0058] Measure EDTA disodium solution with a predetermined concentration of 0.5M, Tris-HCl buffer solution with a pr...

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Abstract

The invention discloses a free DNA preservation liquid, which includes, based on 100 mL, 8-32 g of imidazolidinyl urea, 0.005-0.036 mol of disodium ethylene diamine tetraacetate, 0-2.7 g of glycine, 50-100 [mu]L of Trition X-100, 0-0.005 mol of a pH stabilizer, and 70-85 mL of deionized water. The free DNA preservation liquid can completely preserve free DNA in urine and tissue fluid of the human body for a medium and long time, is suitable for preservation and detection of free DNA of multiple urine and tissue fluid samples, and is wide in application range. The accuracy and credibility of subsequent sample detection is ensured.

Description

technical field [0001] The invention relates to the field of free DNA preservation, in particular to a free DNA preservation solution and its preparation method and application. Background technique [0002] After apoptosis or necrosis, the DNA inside the cell is released into body fluids to form cell-free DNA (cfDNA). The tumor circulating DNA (ctDNA) contained in the cfDNA of cancer patients is closely related to the occurrence and development of tumors. Cell-free DNA is consistent with the gene mutation of tumor tissue, and has rich genetic information; it is positively correlated with tumor burden, and has high detection sensitivity; it responds to the development of the disease at the earliest; with a short half-life (<1.5h), it can realize real-time monitoring. Therefore, cell-free DNA is an important tumor-specific marker, and its complete acquisition and preservation will provide the premise and basis for the application of early cancer screening, stage medicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 章文羿李钰璨黄清瑞李晶陈东海
Owner BEIJING ABACE BIOTECH
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