Novel composite microbial system for degrading straw stalks as well as preparation method and application thereof

A technology of rice straw and composite bacteria, which is applied in the field of new-type composite bacteria for degrading rice straw and its preparation, can solve the problems of complex natural lignocellulose structure and difficulty in decomposing by single microorganisms and enzymes, and achieve high screening efficiency and method Simple and fast degradation effect

Inactive Publication Date: 2017-11-07
宁夏万辉生物环保科技有限公司
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  • Abstract
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Problems solved by technology

However, due to the complex structure of natural lignocellulose in corn stalks, it is difficult to be decomposed by single microorganisms and enzymes

Method used

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  • Novel composite microbial system for degrading straw stalks as well as preparation method and application thereof
  • Novel composite microbial system for degrading straw stalks as well as preparation method and application thereof

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with the examples, and the following examples are intended to illustrate the present invention rather than further limit the present invention, and should not limit the protection scope of the present invention with this.

[0035] 1. Collection of microbial samples

[0036] The collection sites are from surrounding areas of Yinchuan City, Yongning County, Helan County, Dawukou District, Huinong District, Pingluo County of Shizuishan City, Litong District, Hongsibao District of Wuzhong City, Qingtongxia City, Tongxin County, Yanchi County, Guyuan City Yuanzhou District, Xiji County, Longde County, Jingyuan County and Shapotou District, Zhongning County and Haiyuan County of Zhongwei City in Pengyang County, collected the long-term straw returned soil, the soil where straw has been piled up for many years, The decomposed compost and compost-improved soil mainly composed of straw and animal manure were us...

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Abstract

The invention discloses a novel composite microbial system for degrading straw stalks as well as a preparation method and application thereof. The novel composite microbial system comprises bacteria such as Myroides, Ignatzschineria, Wohlfahrtiimonas, Alcaligenes, Bacteroides, Macellibacteroides, Escherichia, Arcobacter and Desulfovibrio. The method comprises the following steps: crushing live earthworms, adding the earthworms and collected microbial samples into a conical flask filled with a fresh selective medium for screening the composite microbial system; screening the selective medium with the highest degradation ability in the composite microbial system; determining strains in the composite microbial system through gene sequencing, and preparing a straw-decomposing inoculants to be applied to degrading the straw stalks. According to the synergistic effects among the strains, cellulose, hemicelluloses and lignin in the straw stalks can be synchronously degraded, and the degradation efficiency is high.

Description

technical field [0001] The invention relates to the technical field of preparation of microbial complex strains for degrading rice straw, in particular to a novel complex strain for degrading rice straw and its preparation method and application. Background technique [0002] As a large agricultural country, my country has the most abundant crop straw resources, and the output of straw can reach more than 700 million tons per year, especially the output of rice straw is the largest. At present, the main way to use rice straw is to return the straw to the field, because the straw can release many nutrients during the decomposition process, thereby improving the soil structure, increasing soil organic matter, increasing soil air permeability, and achieving the purpose of improving soil fertility. [0003] Rice straw is mainly composed of cellulose, hemicellulose, lignin, crude fat, crude protein and ash. The cellulose framework is filled with hemicellulose and lignin, and is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/05C12R1/185C12R1/01
CPCC12N1/20C05F11/08
Inventor 吴庆张学敏董红梅张红王永红张永健尹翠张燕曹云娥田永强
Owner 宁夏万辉生物环保科技有限公司
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