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Novel composite microbial system for degrading maize straws as well as preparation method and application thereof

A technology of corn stalks and composite bacteria, which is applied in the field of new composite bacteria degrading corn stalks and its preparation, can solve the problems of difficult single microorganism and enzyme decomposition, complex structure of natural lignocellulose, etc., and achieve high screening efficiency and method Simpler, easier-to-operate effects

Inactive Publication Date: 2017-11-07
宁夏万辉生物环保科技有限公司
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AI Technical Summary

Problems solved by technology

However, due to the complex structure of natural lignocellulose in corn stalks, it is difficult to be decomposed by single microorganisms and enzymes

Method used

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Examples

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with the examples, and the following examples are intended to illustrate the present invention rather than further limit the present invention, and should not limit the protection scope of the present invention with this.

[0035] 1. Collection of microbial samples

[0036] The collection sites are from surrounding areas of Yinchuan City, Yongning County, Helan County, Dawukou District, Huinong District, Pingluo County of Shizuishan City, Litong District, Hongsibao District of Wuzhong City, Qingtongxia City, Tongxin County, Yanchi County, Guyuan City Yuanzhou District, Xiji County, Longde County, Jingyuan County and Shapotou District, Zhongning County and Haiyuan County of Zhongwei City in Pengyang County, collected the long-term straw returned soil, the soil where straw has been piled up for many years, The decomposed compost and compost-improved soil mainly composed of straw and animal manure were us...

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Abstract

The invention discloses a novel composite microbial system for degrading maize straws as well as a preparation method and application thereof. The novel composite microbial system comprises bacteria such as Myroides, Ignatzschineria, Alcaligenes, Bacteroides, Escherichia, Macellibacteroides, Kerstersia and Arcobacter. The method comprises the following steps: crushing live earthworms, adding the earthworms and collected microbial samples into a conical flask filled with a fresh selective medium for screening the composite microbial system; screening the selective medium with the highest degradation ability through preliminary screening and secondary screening of the composite microbial system; determining strains in the composite microbial system through gene sequencing, and preparing a straw-decomposing inoculants to be applied to degrading the maize straws. The screened composite microbial system is stable. According to the synergistic effects among the strains, cellulose, hemicelluloses and lignin in the maize straws can be synchronously degraded, and the degradation efficiency is high.

Description

technical field [0001] The invention relates to the technical field of preparation of a microbial composite strain for degrading corn stalks, in particular to a novel composite strain for degrading corn straw and its preparation method and application. Background technique [0002] As a large agricultural country, my country has the most abundant crop straw resources, and the annual output of straw can reach more than 700 million tons, especially the largest output of corn straw. At present, the main way to use corn stalks is to return them to the field, because the straws can release many nutrients during the decomposition process, thereby improving soil structure, increasing soil organic matter, increasing soil air permeability, and achieving the purpose of improving soil fertility. [0003] Corn stover is mainly composed of cellulose, hemicellulose, lignin, crude fat, crude protein and ash. The cellulose framework is filled with hemicellulose and lignin, and is connected...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/05C12R1/185C12R1/01
CPCC05F11/08C12N1/20
Inventor 吴庆张学敏董红梅张红王永红张永健尹翠张燕曹云娥田永强
Owner 宁夏万辉生物环保科技有限公司
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