Protein or polypeptide composition and preparation method and use thereof
A composition and protein technology, applied in the field of biochemistry, can solve problems such as research and application that have not been reported.
Active Publication Date: 2017-11-21
上海建华精细生物制品有限公司
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- Abstract
- Description
- Claims
- Application Information
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Problems solved by technology
However, there is no report on the research and application of oligomeric hyaluronic acid and its salts as protein protectors.
Method used
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Embodiment 1
[0046] Preparation of epidermal growth factor (EGF) solution (20μg / ml)
[0047]
Embodiment 2
[0049] Preparation of epidermal growth factor (EGF) solution (0.2 μg / ml)
[0050]
Embodiment 3
[0052] Preparation of basic fibroblast growth factor (bFGF) solution (70 μg / ml)
[0053]
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The invention discloses a composition and a preparation method and a use thereof. The composition contains protein or polypeptide and oligomerization hyaluronic acid (oligomerization HA) or salt of the oligomerization hyaluronic acid; and when being in a liquid form, the protein or polypeptide is 0.1-100 [mu]g / ml, and the oligomerization hyaluronic acid or the salt of the oligomerization hyaluronic acid is 1-20%.
Description
technical field [0001] The present invention relates to the field of biochemistry, in particular to a protein or polypeptide composition and its preparation method and application. Background technique [0002] In 1934, Meyer et al. extracted and isolated a macromolecular polysaccharide from the vitreous humor of the bull's eye, and named it hyaluronic acid. Hyaluronic acid (hyaluronan, HA) is a macromolecular linear glycosaminoglycan (GAG), which consists of repeating disaccharide units, namely D-glucuronic acid (β1-3) and N-acetyl-D- Glucosamine (β1-4). HA is widely present in organisms and has a variety of biological activities. It serves as the mechanical support of cells in many tissues such as skin, tendon, muscle and cartilage. It is the main component of the intercellular matrix and plays an important role in the moistening and lubrication of tissues. effect. [0003] HA and the respective salts have been used as medicine, especially in the treatment of joint dise...
Claims
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IPC IPC(8): A61K38/18A61K38/27A61K47/26A61K47/36A61K8/73A61K8/64A61K8/60A61Q19/00
CPCA61K8/60A61K8/64A61K8/735A61K38/18A61K38/1808A61K38/1825A61K38/1875A61K38/27A61K47/26A61K47/36A61Q19/00
Inventor 周庆玮郭学彦杜鹏
Owner 上海建华精细生物制品有限公司
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