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Use of combination of chemical agents and biocontrol microbes for preventing and controlling continuous cropping tomato bacterial wilt

A technology of tomato bacterial wilt and microbial strains, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, to achieve significant biological control, improved control effects, and significant synergistic effects

Inactive Publication Date: 2017-11-21
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, there is no effective chemical pesticide to control tomato bacterial wilt, and there is an urgent need to develop new technologies for the control of tomato bacterial wilt

Method used

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  • Use of combination of chemical agents and biocontrol microbes for preventing and controlling continuous cropping tomato bacterial wilt
  • Use of combination of chemical agents and biocontrol microbes for preventing and controlling continuous cropping tomato bacterial wilt
  • Use of combination of chemical agents and biocontrol microbes for preventing and controlling continuous cropping tomato bacterial wilt

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Experimental program
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Embodiment Construction

[0027] (1) Isolation and identification of strains

[0028] Bacteria were isolated from the rhizosphere soil of tomato plants by the plate dilution method, and then the antagonistic actinomycetes were screened by the confrontation method with Ralstonia solanacearum (Ralstonia solanacearum), the pathogen of tomato bacterial wilt, as the indicator bacteria. Finally, the high-efficiency antagonistic strain B8 was screened out, and the strain B8 was identified as Bacillus amyloliquefaciens through morphological characteristics, physiological and biochemical characteristics and 16S rRNA gene sequence.

[0029] The strain B8 was deposited on April 17, 2017 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the strain preservation number is CGMCC NO.14042. The main biological characteristics are: when F8 grows on LB medium, the colony is gray and round, and the colony is smooth at the beginning, and there are wrinkles on the surface of...

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Abstract

The invention discloses a use of combination of chemical agents and biocontrol microbes for preventing and controlling continuous cropping tomato bacterial wilt. An antagonistic bacterium strain (bacillus B8) having obvious antagonistic effects on tomato bacterial wilt is separated. The strain B8 belongs to Bacillus amylolique Baciens, is preserved in the China general microbiological culture collection center on April 17, 2017 and has a preservation number CGMCC NO. 14042. The antagonistic bacterium strain is used for producing a liquid inoculant and microbial organic fertilizer, the liquid inoculant has antagonistic bacterium quality of greater than 10<9> / ml and the microbial organic fertilizer has antagonistic bacterium quality of greater than 5.0*10<7> / g. The antagonistic bacterium liquid inoculant, the microbial organic fertilizer containing the antagonistic bacterium strain and 0.3%(W / V) of a potassium phosphite solution are combined and used in the soil for tomato continuous cropping and have a tomato bacterial wilt prevention and treatment rate of 70% or more.

Description

technical field [0001] The invention relates to the joint prevention and control of tomato bacterial wilt by using chemical agents, antagonistic antibacterial agents and microbial organic fertilizers, and belongs to the field of comprehensive prevention and control of plant soil-borne diseases. Background technique [0002] Tomato bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum. The incidence rate is usually 20%-30%. It is especially serious in continuous cropping. Sometimes the incidence rate is as high as 70-80%, or even crop failure . At present, there is no effective chemical pesticide to control tomato bacterial wilt, and it is urgent to develop new technologies for the control of tomato bacterial wilt. We found that potassium phosphite (KH 2 PO 3 ), can be used as phosphorus and potassium fertilizer, and has the ability to inhibit the growth of pathogenic bacteria - R. solanacearum at a certain concentration. Potassium phosphite and antagonis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05G3/00C12R1/07
CPCC05B17/00C05F11/08C05G3/00C12N1/205C12R2001/07C05C11/00C05F3/00C05F17/00Y02W30/40
Inventor 沈标苏律杨兴明邱鹏飞沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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