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A kind of poplar rooting medium and poplar rooting transplanting seedling training method

A rooting culture medium and culture medium technology, applied in the direction of culture medium, biochemical equipment and methods, gardening methods, etc., can solve the problems that agar is difficult to clean up, and achieve the goals of ensuring normal rooting and growth, reducing costs, and reducing mortality Effect

Active Publication Date: 2019-08-23
INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The poplar rooting medium uses a mixture of vermiculite, field soil and peat soil as a solidifying agent, replacing the agar in the prior art, and solving the problem that the agar is difficult to clean when transplanting seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of poplar rooting transplanting seedling training method, comprises the following steps:

[0034] A, first-generation culture: inoculate the tender stems of "Mengshu No. 1 poplar" poplar as explants on the first-generation culture medium, and cultivate them for 35 days at 23°C, with a light intensity of 2000Lux and 10 hours of light time per day, to obtain The first generation of sterile vaccine;

[0035] The first generation culture medium is the MS medium that has added sucrose, 6-KT, NAA and agar, the concentration of sucrose in the first generation culture medium is 20g / L, the concentration of 6-KT is 1.5mg / L, and the concentration of NAA 0.03mg / L, the concentration of agar is 6g / L.

[0036] B, subculture: Get the adventitious buds induced on the first-generation aseptic seedlings obtained in step A, transfer the adventitious buds to the subculture medium for 25 days of proliferation and culture, and then 3.5cm downwards from the top of the buds Cut off at ...

Embodiment 2

[0044] A kind of poplar rooting transplanting seedling training method, comprises the following steps:

[0045] A, primary culture: Inoculate poplar tender stems as explants on the primary culture medium, cultivate them for 25 days at 27° C., light intensity 1600 Lux, and 16 hours of light per day, to obtain the primary aseptic seedlings;

[0046] The first generation culture medium is the MS medium that has added sucrose, 6-KT, NAA and agar, the concentration of sucrose in the first generation culture medium is 30g / L, the concentration of 6-KT is 0.5mg / L, and the concentration of NAA 0.07mg / L, the concentration of agar is 5g / L.

[0047] B, subculture: Get the adventitious buds induced on the first-generation aseptic seedlings obtained in step A, transfer the adventitious buds to the subculture medium for 35 days after proliferation and culture, from the top of the buds 2.5cm downwards Cut off at the place, and use the cut off part as subculture sterile vaccine;

[0048] Descr...

Embodiment 3

[0055] A kind of poplar rooting transplanting seedling training method, comprises the following steps:

[0056] A, first-generation culture: inoculate poplar tender stems as explants on the first-generation culture medium, cultivate for 30 days at 25° C., light intensity 1800 Lux, and 13 hours of light time per day, to obtain the first-generation aseptic seedlings;

[0057] The first generation culture medium is the MS medium that has added sucrose, 6-KT, NAA and agar, the concentration of sucrose in the first generation culture medium is 25g / L, the concentration of 6-KT is 1.0mg / L, and the concentration of NAA 0.05mg / L, the concentration of agar is 5.5g / L.

[0058] B, subculture: Get the adventitious buds induced on the first-generation aseptic seedlings obtained in step A, transfer the adventitious buds to the subculture medium for 30 days of proliferation and culture, and then place it 3cm downwards from the top of the buds Truncated, the cut off part is used as subculture...

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Abstract

The invention provides a poplar rooting medium and a poplar rooting transplanting and seedling hardening method. In the poplar rooting medium, the mixed soil consisting of vermiculite, field soil and turfy soil is adopted as a curing agent which replaces agar in the prior art, and the problem that the agar is hard to clear away in seedling transplanting is solved. The poplar rooting transplanting and seedling hardening method adopts the poplar rooting medium, much time for cleaning the medium is saved in the transplanting process, and the root of a plant is protected from damage, thereby ensuring normal rooting and growth of seedlings; the death rate of the seedlings in the transplanting and seedling hardening process is effectively reduced, and the time and cost are effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of poplar propagation and specifically relates to a poplar rooting medium and a method for poplar rooting, transplanting and training seedlings. Background technique [0002] Poplar has strong adaptability to the environment and is widely distributed in my country. It can grow well no matter it is fertile land or barren mountainous area. Poplar has high economic use value and environmental protection value. Its trunk is straight, its material is fine and dense, and it grows fast. It is one of the important tree species in pulpwood raw material forest, and it is also one of the afforestation tree species in excellent water conservation forest and main shelter belt. [0003] At present, the commonly used rooting medium in poplar tissue culture is to add agar powder as a solidifying agent. One of the biggest problems with the agar solidifying agent is that it is difficult to clean up when transplanting seedling...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00A01G17/00A01G24/15A01G24/10A01G24/28
Inventor 李奕稹
Owner INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD
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