Method for producing pelargonium hortorum bailey skin care enzyme by utilizing honey source bacteria

A technology of geranium and honey-derived bacteria, applied in the field of bioengineering, can solve the problems of inability to retain enzyme activity for a long time, inability to ensure no pathogenic bacteria, long fermentation time, etc., and achieve the effects of inhibiting the growth of miscellaneous bacteria, retaining enzyme activity and stable product quality.

Inactive Publication Date: 2017-12-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Provides a preparation method that can keep the nutrition and health care ingredients of geranium enzyme to the greatest extent, and solves the problems that the existing enzyme pr

Method used

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Examples

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Example Embodiment

[0024] Example 1

[0025] A method for fermenting geranium enzyme, including the following steps:

[0026] (1) Choose 500g of fresh geranium as the main raw material for fermentation.

[0027] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, and put it into a cleaned container with pure water, about 20% of the volume, add 10% of the total liquid volume % Of raw honey is used as fermentation stock for later use.

[0028] (3) Prepare LB medium, activate the original preserved strain, and culture the fermentation broth obtained for about 48 hours for use;

[0029] (4) According to the inoculation ratio of 7%, nectar bacteria and lactobacilli were respectively taken into the fermentation stock solution, and the fermentation was carried out for 9 days with a pH value of 7.0 and a temperature of 28°C.

[0030] (5) Carry out coarse filtration first, and the number of filter sieves is 20-32 mesh. Then finely filter, the number of...

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Abstract

The invention provides a method for making geranium skin care enzymes with nectar-derived bacteria. The technological feature is to use geranium as the main raw material, and the preservation number preserved in the China Typical Culture Collection Center on June 24, 2013 is: CCTCC NO: M2013283 Bacillus amyloliquefaciens H47 was the fermentation strain of Bacillus amyloliquefaciens. According to the volume ratio of geranium: raw honey: pure water is 2‑5: 1‑3: 7‑2, add it and mix it evenly to make a geranium fermentation stock solution, add the nectar source bacteria seed liquid to the fermentation stock solution according to the ratio of 1‑5%. After fermenting for 6-14 days at a temperature of 25°C-35°C and a pH value of 5.0-8.0, the crude product of geranium skin care enzyme can be obtained. Preliminarily screen out large particle residues through a coarse filter with a number of 20-32 meshes, and then further filter out small particle residues through a fine filter with a number of 80-100 meshes to obtain the geranium skin care enzyme.

Description

technical field [0001] The invention relates to an enzyme production method, in particular to a fermentation method of geranium skin care enzyme, and belongs to the technical field of bioengineering. Background technique [0002] Enzyme is a Japanese name for enzyme (enzyme), and later the initial biologically active products obtained by microbial fermentation are collectively referred to as enzymes. However, because many producers do not pay attention to the use of fermenting microorganisms and wild functional materials, the development and application of enzyme products are affected by their side effects and poor functionality. Through a large number of screening and experiments, the present invention obtains a green and healthy enzyme production method with obvious effect. [0003] Geranium, as a worldwide flower, not only has extremely high garden ornamental value, can improve the living environment, has high ornamental value, but also is an important medicine and chemi...

Claims

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Application Information

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IPC IPC(8): A23L33/00
CPCA23V2002/00
Inventor 廖祥儒张永陈佳君蔡宇杰管政兵刘海燕明经林
Owner JIANGNAN UNIV
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