A kit for detecting alpha and beta globin gene sequences in one tube

Abstract

The invention discloses a kit for detecting α and β globin gene sequences in one tube, including amplification primers, and used for identifying α globin gene sequences -SEA, -α 3.7 ,-α 4.2 Site deletions, alpha globin gene sequence CD30, CD31, CD59, WM, QS, CS site mutations and beta globin gene sequence ‑82, ‑80, ‑79, ‑78, Cap+1, Cap40‑43, Int , CDs14 / 15, CD17, CD19, CD26, CDs27 / 28, CD30, IVSⅠ‑1, CD31, CD37, CDs41 / 42, CD43, CDs71 / 72, CD95, IVSⅡ‑5, IVSⅡ‑654 site mutation probe .

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a kit for detecting alpha and beta globin gene sequences in one tube. Background technique

[0002] Thalassemia (thalassemia, referred to as thalassemia) is a kind of monogenic genetic hemolytic disease caused by the imbalance of globin chain synthesis. This disease was first discovered in the peoples along the Mediterranean Sea, and it is one of the most common blood genetic diseases that have the greatest impact on human health in the world. The globin in normal adult hemoglobin is composed of 4 peptide chains, namely α, β, γ, and δ chains. Each chain has a corresponding gene code, and point mutations, deletions or insertions of these genes will cause the peptides to varying degrees. Chain synthesis disorders, resulting in changes in hemoglobin components, resulting in anemia.

[0003] Thalassemias are usually divided into four types: α, β, δβ, and δ, of which α and β thalassemia...

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