Rapid detection method for whether raw fresh milk is qualified
A detection method and technology for raw milk, applied in the field of pathogen detection, can solve the problems of long detection time, complicated technical operation, difficult popularization and application, etc., and achieve the effects of simple operation, low price, and no pollution.
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[0022] Below, the applicant will further describe the method of the present invention in detail in combination with specific embodiments and accompanying drawings.
[0023] 1. Isolation and identification of contaminating microorganisms in fresh milk.
[0024] Isolation of main pathogenic bacteria: The fresh milk comes from the first two handfuls of milk from the dairy farm, a total of 60 parts. Take raw milk samples and put them on nutrient agar solid medium, 10% fresh rabbit fiber blood nutrient agar medium, potato solid (PDA) medium, trypticase soybean broth (TSB) medium, LB liquid medium plate respectively Streak directly, culture at 37°C for 24-48 hours, carry out aerobic isolation culture, observe and isolate a single colony, record its characteristics, and continue to identify. At the same time, directly inoculate fresh milk samples in LB liquid medium, cover with liquid paraffin, incubate at 37°C for 24h, inoculate on nutrient agar solid medium, and incubate at 37°C f...
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