Micro-fluidic chip and chip assembly

Abstract

The invention discloses a micro-fluidic chip and a chip assembly, and relates to the technical field of microfluidics. Integration and fully integrated operation of sample preparation and amplification separation in nucleic acid detection can be realized, operation of the nucleic acid is facilitated, and the nucleic acid detection efficiency is improved. The micro-fluidic chip comprises a liquid storage unit, an extraction and amplification unit as well as a waste liquid recovering unit, wherein the liquid storage unit is used for realizing sequential sampling of liquid reagents; the extraction and amplification unit is used for performing amplification and separation to obtain a to-be-detected sample; the waste liquid recovering unit is used for recovering the detection waste liquid; theliquid storage unit comprises a plurality of liquid storage devices and a first circulating pipeline communicated with the liquid outlet of each liquid storage device; a first control piece is arranged between each liquid outlet and the first circulating pipeline; the first control piece is used for controlling the breakover state of the liquid outlet; when any one liquid storage device is in a pressurizing state, the corresponding first control piece is in an opening state and the rest first control pieces are in a closed state. The micro-fluidic chip and the chip assembly, which are providedby the invention, are applied to nucleic acid detection.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip and a chip component. Background technique [0002] Microfluidic chip technology refers to the integration of basic operations such as nucleic acid sample preparation, amplification and separation, and heating detection in the process of biological, chemical, and medical analysis into a micron-scale chip, which can automatically complete the entire analysis process. Integrated technology. However, since the sample preparation process needs to strictly control the sequential injection of multiple reaction reagents, it is not easy to implement in a microfluidic chip with a small volume and a complex structure. Therefore, the microfluidic chip in the prior art can only realize the Nucleic acid amplification, separation and heating detection, while the sample preparation process needs to be carried out in a separate external device, for example, the sample ...

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Problems solved by technology

However, since the sample preparation process needs to strictly control the sequential injection of multiple reaction reagents, it is not easy to implement in a microfluidic chip with a small volume and a complex structure. Therefore, the microfluidic chip in the prior art can only realize the Nucleic acid amplification, separation and heating detection, while the sample preparation process needs to be carried out in a separate external device, for example, the sample preparation is completed using an external storage tank, and then the obtained sample is connected to the microfluidic chip through a hose, so that After the microfluidic chip obtains the sample, it completes operations such as amplification separation, heating detection, etc.

[0003] Although the method of externally connecting the liquid storage tank can better solve the problem of sequentially injecting multiple reaction reagents, there are also deficiencies in actual operation, because the interface design of the microfluidic chip is very small, so every time the hose is connected Time-consuming and labor-intensive, which leads to complex operation process of nucleic acid detection and low detection efficiency

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