Artificial rearing method of hepialidae and feed composition of hepialidae larvae
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A feed formula, bat moth technology, applied in the direction of animal feed, animal husbandry and the like, achieves the effects of simple preparation method, improved pupa eclosion rate, and high eclosion rate
Active Publication Date: 2018-01-09
HUNAN NORMAL UNIVERSITY
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There has not been any report on the artificial breeding method and artificial feed of the host insect bat moth of Cordyceps xuefeng
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Embodiment 1
[0049] (1) Materials and methods
[0050] 1.1 The source of the tested larvae
[0051] Healthy larvae of the giant furuncle moth with similar body length and weight were selected and reared indoors with fresh carrots for 10 days before being tested.
[0052] 1.2 Artificial Feed Raw Materials
[0053] Daqing powder: the Daqing root collected from the place of origin of the giant boil moth, dried naturally, crushed by a miniature plant sample grinder (Beijing Kewei Yongxing Instrument Co., Ltd., model: FZ102), and passed through a 40-mesh sieve.
[0054] Ascorbic acid (Xilong Chemical Co., Ltd.) (i.e. vitamin C);
[0055] Carrot powder (buy fresh carrots, cut into small pieces, diameter <1mm);
[0056] Sorbic acid (produced by Sinopharm Chemical Reagent Co., Ltd.);
[0057] Oatmeal, soybean flour, and corn flour are obtained in bulk from supermarkets or by purchasing dried soybeans and corn flour;
[0114] 1.1 Observation and identification method of female and male moths
[0115] Take giant furuncle bat moth pupae, put them under a stereoscope, observe the morphological characteristics of the end of the abdomen, and take pictures and number them. Pupae are divided into different types according to their morphology. Until the pupa emerges, the sex of the pupa is confirmed backwards according to the sex of the adult.
[0116] Put the pupae directly into an empty tissue culture bottle, or prepare a 2.5% agar (containing 0.4% preservative) mixture and pour it into the tissue culture bottle, then put the pupae into the bottle, and cover with a ventilating cap; When the color turns dark brown, move the pupae into an empty tissue culture bottle, put a clean filter paper under the pupae, and fold up the upward side of the filter paper for the adult worms to cling to. The relative humidity is 80±2%, and the temperature is 19~22℃.
[0117] 1.2 Conditions f...
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Abstract
Disclosed are an artificial rearing method of hepialidae and a feed composition of hepialidae larvae. The artificial rearing method of hepialidae meets the following conditions of 1, artificial feeding condition of the larvae, wherein the temperature is 8-23 DEG C, the relative humidity is 75-85%, aseptic water is adopted for humidification, and an overall dark environment is provided; 2, the storage conditions of pupae, wherein female and male pupae which pupate at the same day are placed at different temperatures; 3, mating and spawning conditions of imagoes, wherein female and male imagoesafter eclosion are placed in an imago cage which is provided with a gauze screen and internally contains fresh host plant indigowoad leaves in a bottled mode, the imagoes can stay in a suspended mode,move, courtship, mate and spawn in the cage, the temperature is 23-25 DEG C, the relative humidity is 65-80%, and the photoperiod is (11-13)L:(13-11)D; 4, the artificial feed composition of the hepialidae larvae and a preparation method thereof; 5, disinfection treatment of the rearing environment. By means of the artificial rearing method of hepialidae and the feed composition of the hepialidaelarvae, the survival rate of the larvae can be significantly increased, the eclosion rate of the pupae can be increased, and the success rate of imago mating is increased.
Description
technical field [0001] The invention relates to an artificial feeding method of bat moths and a larvae feed formula thereof. Background technique [0002] Cordyceps Xuefeng is a precious Chinese herbal medicine newly discovered in Dongkou County and other places in the Xuefeng Mountains of Hunan Province. It is a new fungal group of Cordyceps Xuefeng. Ophiocordyceps xuefengensis The combination of insects and bacteria formed after parasitic insects, research found that it contains adenosine, cordycepin, cordycepic acid, polysaccharides, amino acids and other active substances, has the effects of nourishing the lungs and kidneys, relieving cough and reducing phlegm, and enhancing immunity. Yao medicine has a long history of use. [0003] Important hosts of Cordyceps xuefeng include the giant boiled bat moth Endoclita Davidi (Poujade, 1886) and the boil bat moth Endoclyta nodus (Chu et Wang, 1985) and other Lepidoptera bat moths (Hepialidae) bat moths ( Endoclita ) Ins...
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