Tissue culture rapid propagation technology for morinda officinalis

A technology for tissue culture rapid propagation and Morinda officinalis, which is applied in the field of plant tissue culture, can solve problems such as reducing the yield of medicinal materials, and achieve the effects of saving seeds and increasing yield.

Inactive Publication Date: 2018-01-16
陈培党
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Morinda officinalis is mainly propagated by seeds, but its medicinal part is also seeds, which reduces the yield of its medicinal materials to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Obtaining sterile seedlings: select plump Morinda officinalis seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, disinfect with 75% alcohol for 38 seconds, then disinfect with 0.1% mercury solution for 6 minutes, and finally use sterile Rinse twice with water, dry the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, it was placed in the light for 22 hours a day, the light intensity was 800lx, the culture temperature was 24°C, and the relative air humidity was 75% and the germination rate reached 100% after 18 days of cultivation. The germination medium is: 1 / 2MS+0.01mg / L NAA+12g / L sucrose+3.2g / L agar, with a pH of 5.4.

[0015] (2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.3 cm with a scalpel, and inoculate them in the medium for callus induction t...

Embodiment 2

[0021] (1) Acquisition of sterile vaccines: select plump Morinda officinalis seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, sterilize with 75% alcohol for 46 seconds, then sterilize with 0.1% mercury solution for 9 minutes, and finally use sterile Rinse with water for 6 times, blot the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, it is placed in the light for 25 hours a day, the light intensity is 1600lx, the culture temperature is 26°C, and the relative air humidity is 75%. After 21 days of cultivation, the germination rate reaches 100%. The germination medium is: 1 / 2MS+0.06mg / L NAA+19g / L sucrose+3.9g / L agar, with a pH of 5.8.

[0022] (2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.6 cm with a scalpel, and inoculate them in the medium for callus induct...

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PUM

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Abstract

The invention discloses a tissue culture rapid propagation technology for morinda officinalis. The morinda officinalis is a rubiaceae plant, can be harvested in the whole year and can be preferably harvested in autumn and winter. The technology comprises the following steps: digging the roots of 5-7 years old morinda officinalis; removing lateral roots; cleaning and drying to 60%-70% dry state; lightly flattening by using a mallet; and drying in sun and cutting into 6-12cm long roots, thereby acquiring an end product. If the end product is steamed for a half hour or is soaked in boiled water,the color is purple and the quality is higher; the roots are in flat cylindrical or net cylindrical shape, are slightly bent and have longitudinal wrinkles and deep sunken horizontal grains; the surface is in dark gray or brown color and is in slight purple color; the texture is tough and the broken section is uneven; the roots are odorless and taste sweet and slightly astringent; and the optimalroots have characterstics of dry stem, toughness, bead string shape, thick pulp and purple color. According to the invention, the morinda officinalis seeds are taken as explants; the morinda officinalis isolated plant is successfully acquired through the processes of callus induction, proliferation, differentiation, rooting, hardening-seedling transplanting, and the like; a tissue culture rapid propagation technical system for morinda officinalis is established; the seed usage in the cultivation production is saved; and the yield of the medicinal material thereof is increased.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a technique for tissue culture and rapid propagation of Morinda officinalis. Background technique [0002] Morinda officinalis is a Rubiaceae plant, produced in Guangdong and Guangxi, and can be harvested throughout the year, preferably in autumn and winter. Dig out the roots that are 5-7 years old, remove the lateral roots, wash them, dry them in the sun until they are 60-70% dry, flatten them gently with a mallet, then dry them in the sun, and cut them into 6-12cm long to get the finished product. If steamed for half an hour or soaked in boiling water, the color will be more purple and the quality will be better. Morinda officinalis is flat cylindrical or net-cylindrical, slightly curved, with longitudinal wrinkles and deep horizontal stripes, some constricted or the outer skin is transversely broken to expose the water center, forming a bead shape, varying in length, diameter 1 ~2cm; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 陈培党
Owner 陈培党
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