Tissue culture rapid propagation technology for morinda officinalis
A technology for tissue culture rapid propagation and Morinda officinalis, which is applied in the field of plant tissue culture, can solve problems such as reducing the yield of medicinal materials, and achieve the effects of saving seeds and increasing yield.
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Embodiment 1
[0014] (1) Obtaining sterile seedlings: select plump Morinda officinalis seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, disinfect with 75% alcohol for 38 seconds, then disinfect with 0.1% mercury solution for 6 minutes, and finally use sterile Rinse twice with water, dry the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, it was placed in the light for 22 hours a day, the light intensity was 800lx, the culture temperature was 24°C, and the relative air humidity was 75% and the germination rate reached 100% after 18 days of cultivation. The germination medium is: 1 / 2MS+0.01mg / L NAA+12g / L sucrose+3.2g / L agar, with a pH of 5.4.
[0015] (2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.3 cm with a scalpel, and inoculate them in the medium for callus induction t...
Embodiment 2
[0021] (1) Acquisition of sterile vaccines: select plump Morinda officinalis seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, sterilize with 75% alcohol for 46 seconds, then sterilize with 0.1% mercury solution for 9 minutes, and finally use sterile Rinse with water for 6 times, blot the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, it is placed in the light for 25 hours a day, the light intensity is 1600lx, the culture temperature is 26°C, and the relative air humidity is 75%. After 21 days of cultivation, the germination rate reaches 100%. The germination medium is: 1 / 2MS+0.06mg / L NAA+19g / L sucrose+3.9g / L agar, with a pH of 5.8.
[0022] (2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.6 cm with a scalpel, and inoculate them in the medium for callus induct...
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