Preparation method and antioxidation application of ethanol extract of flowers of Apios americana Medikus

Abstract

The invention discloses a preparation method for the ethanol extract of the flowers of Apios americana Medikus. The preparation method comprises the following steps: 1) mixing the flowers of Apios americana Medikus with ethanol containing hydrochloric acid with a concentration of 0.1 mol / L, successively carrying out smashing and ultrasonic extraction, and subjecting obtained extract to centrifugation to obtain a supernatant; 2) subjecting the supernatant to rotary evaporation and concentration until the volume of the supernatant is reduced by 80 to 90%; 3) subjecting a concentrate obtained inthe step 2) to separation and purification via macroporous resin with an aqueous formic acid solution with a volume concentration of 1% as a rinsing agent and an aqueous methanol solution containing 0.1% of formic acid as an eluting agent, and collecting eluate; 4) subjecting the eluate to rotary evaporation and concentration until the volume of the eluate is reduced by 80 to 90% so as to obtain slurry-like concentrate; and 5) pre-freezing the slurry-like concentrate, and then drying the pre-frozen slurry-like concentrate into powder with a vacuum lyophilizer so as to obtain the ethanol extract of the flowers of Apios americana Medikus. The invention also discloses application of the ethanol extract of the flowers of Apios americana Medikus to preparation of inhibitors for oxidative damageto hepatocytes.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of ethanol extract of taro flower and its anti-oxidation application. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among them, taro f...

AI Technical Summary

Problems solved by technology

Therefore, at present, many nutrition and health food researchers are very concerned about the research and topic of anti-oxidation and the occurrence and prevention of chronic diseases, but there are few reports on the antioxidant activity of taro flower extract

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