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Method for constructing pistillate line recombinant inbred line group for balsam pear

A construction method and technology of inbred lines, applied in the field of construction of recombinant inbred line populations of bitter melon strong female lines, can solve the problems of weak group integrity and low retention rate of individual plants, and achieve the effect of strong integrity and simple operation

Inactive Publication Date: 2018-01-19
VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] The present invention aims at the technical defect of prior art, provides a kind of construction method of bitter melon strong female line recombinant inbred line population, to solve the bitter melon strong female line recombinant inbred line ( In RI) populations, the technical problems of low individual plant retention rate and weak population integrity

Method used

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Embodiment Construction

[0034] Specific embodiments of the present invention will be described in detail below. In order to avoid too many unnecessary details, well-known structures or functions will not be described in detail in the following embodiments. Unless defined otherwise, technical and scientific terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0035] The test reagent consumables used in the following examples are conventional biochemical reagents unless otherwise specified; the experimental methods are conventional methods unless otherwise specified; the quantitative tests in the following examples are all set to repeat the experiment three times, The results are average values; % in the following examples, unless otherwise specified, are mass percentages.

[0036] Take the construction of a recombinant inbred line population as an example by taking the bitter melon strong female line Q...

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Abstract

The invention provides a method for constructing a pistillate line recombinant inbred line group for balsam pear. The technical scheme comprises following steps: group construction base requirement, sowing time, seedling growing, soil preparing and fertilizing, field planting, fertilizing and watering management, frame building and pruning, artificial pollination and insect and disease controlling, seed collecting and seed storing. By means of single plant self-cross, plants from F2 to F6 are all preserved as possible; the characteristic of single plant is kept completed. By means of the method, a large enough recombinant inbred line group from F2 to F6 is obtained; the advantage of the recombinant inbred line group (RI) is that it can be used for long time and can be tested repeatedly. Therefore, the recombinant inbred line group can be used for constructing molecule mark linkage and mapping of quantitative trait loci (QTL). By means of the method, single plant in the pistillate linerecombinant inbred line group for balsam pear is kept as possible; the method is easy to operate and the group has stronger group integrity.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for constructing a recombinant inbred line population of bitter melon strong female lines. Background technique [0002] Bitter melon (Momordica charantia L.), a trailing annual plant of the family Cucurbitaceae Momordica, originated in tropical and subtropical regions, and is widely distributed in the Amazon region of South America, Africa, Asia, the Caribbean and other places. The region is developing rapidly. Because bitter gourd is rich in vitamin C, bitter glycosides and bitter gourd (bitter gourd is known as the "fat killer" can reduce fat and polysaccharides), a substance similar to insulin (polypeptide-P) and a protein lipid Substances (stimulating and enhancing the ability of immune cells in animals to devour cancer cells, it can play an anti-cancer effect in the body together with quinine in alkaloids), so that it has the functions of clearing heat and n...

Claims

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Application Information

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IPC IPC(8): A01G22/05A01G24/28A01G24/20A01H1/02
Inventor 张景云万新建关峰缪南生
Owner VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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